Browsing by Author "Ünlü, A"

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    Patients with urinary incontinence often benefit from surgical detethering of tight filum terminale
    Selcuki, M; Ünlü, A; Ugur, HC; Soygür, T; Arikan, N; Selcuki, D
    We retrospectively reviewed 77 patients with a tethered spinal cord syndrome to evaluate the results of neurosurgical treatment. The patients were divided into two groups: in group 1 there were 17 patients with primary tethered cord who had normal level conus medullaris (NLCM) and normal thickness filum terminale (NTFT) with urinary incontinence, and group was made up of 60 patients with secondary spinal cord tethering after a previous closure of a midline fusion defect who had a low-lying conus medullaris. Neurological examination, radiography, urodynamic tests and electrophysiological findings confirmed the diagnosis. Conventionally, tethered cord syndrome has been defined as a state in which the conus medullaris is located below the L1-2 disc space. However. in a patient with urinary incontinence and a hyper-reflexive type of neurogenic bladder, in whom the conus medullaris is found to be at the normal level, there may still be cord tethering that is causing the incontinence. In this study the comparison was based on evaluation of the response to treatment and general characteristics of the syndrome in both groups of patients to draw attention to the general approach to this incapacitating maldevelopment.
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    Comparative phytochemical studies on the roots of Polygala azizsancarii and P. peshmenii and neuroprotective activities of the two xanthones
    Çalis, I; Becer, E; Ünlü, A; Aydin, ZU; Hanoglu, A; Vatansever, HS; Dönmez, AA
    Six known sucrose mono-, di- and triesters and five xanthone derivatives were isolated from the roots of Polygala peshmenii Eren, Parolly, Raus & Kurschner which is a narrow species endemic to Turkiye. Among the xanthones, 1,7-dihydroxy-2,3-methylenedioxy-5,6-dimethoxy-xanthone is an undescribed compound isolated for the first time from a natural source. The studies on the roots of P. azizsancarii Do & BULL;nmez have resulted in the isolation of four known compounds including sucrose mono-, di- and triesters. The structures of the sucrose esters and xanthones isolated from P. azizsancarii and P. peshmenii were established by spectroscopic methods, including 1DNMR (1H NMR, 13C NMR, DEPT-135), 2D-NMR (COSY, NOESY, HSQC, HMBC). Neuroprotective activities of two xanthones, 1,3,6-trihydroxy-2,5,7-trimethoxyxanthone and 3-O-& beta;-D-glucopyranosyloxy-1,6-dihydroxy-2,5,7-trimethoxyxanthone isolated from the roots of P. azizsancarii were evaluated in vitro using in a cellular model of Alzheimer's disease. SKNAS human neuroblastoma cells were used in the study and treated with different consecrations of A & beta;25-35 oligomer for up to 48 h. Cell viability was evaluated using MTT assay. The distribution of & beta;-amyloid, & alpha;-synuclein, tau, JAK2, STAT3, caspase 3 and BMP-2 were investigated using indirect immunoperoxidase staining. Our results suggested that both xanthones control tau aggregation with no effect on & beta;-amyloid plaque formation. In addition, for neuronal pathophysiology in AD cell model, decreased distributions of JAK/ STAT3 and BMP2 signaling pathways were demonstrated, therefore they play a role in the protective effect on neurons in neurodegenerative disease. A significant decrease in caspase 3 immunoreactivity was detected after the administration of both compounds in AD cells. Therefore, both compounds control neuronal pathophysiology and rescue cell death in AD disease.
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    Sera levels of homoarginine, homocysteine and ischemia modified albumin in patients with breast cancer
    Kosova, F; Abusoglu, S; Beksaç, ÖK; Tekin, G; Çetin, B; Dinçel, AS; Ari, Z; Ünlü, A
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    The Investigation of Treatment Effects on Serum Biochemical Parameters in Bladder Cancer Diseases
    Üçer, O; Kosova, F; Temeltas, G; Abusoglu, S; Ünlü, A; Dinçel, AS; Ari, Z
    Objective: The most common cancer in smokers is bladder cancer (BC). Intravesical chemo immunotherapies are used to lower the risk of progression in patients who are at risk. Bacillus Calmette-Guerin immunotherapy is the most effective adjuvant therapy discovered. We aimed to evaluate the levels of arginine, citrulline, ornithine, symmetrically dimethylated arginine (SDMA), N-monomethyl-Larginine. (L-NMMA) or asymmetrically dimethylated arginine (ADMA) in patients with BC, as well as their relationship with methylarginine. Materials and Methods: Blood samples were collected from all patients (n=30) and controls (group 1) prior to transurethral resection of bladder tumour (TURBT) (group 2), 20 days after TURBT (group 3) and at the end of intravesical immunotherapy 74 (group 4). The levels of serum methylated arginine were measured using ABSCIEX API 3200 tandem mass spectrometry system in positive ESI mode. Results: In comparison to group 2, group 1's ADMA and arginine/total methylated arginine levels were 98 significantly lower (p=0.035 and p=0.049, respectively), while SDMA/ADMA, L-NMMA and arginine/ADMA levels (p=0.001, p=0.008 and p=0.017) increased, and no statistical difference was found for other parameters (p>0.05). When compared to group 3, ADMA, arginine, citrulline, methylated arginines and L-NMMA levels in group 2 (p=0.035, p=0.001, p=0.015, p=0.032, p=0.032) increased, while SDMA/ADMA levels (p=0.041) decreased. Conclusion: The decrease in arginine and ADMA levels in non-muscle invasive BC patients is thought to be promising, and these markers may be useful in monitoring the diagnosis and treatment of patients.
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    The cytotoxic and antiproliferative effect of Polygala saponin XLIV on the human colorectal carcinoma cell line
    Becer, E; Hanoglu, A; Ünlü, A; Aydin, ZU; Dönmez, AA; Jurt, S; Vatansever, SH; Çalis, I
    Objectives Saponins are secondary metabolites naturally found in plants with diverse pharmacological properties such as anticancer. This research aimed to explore the anti-cancer properties of Polygalasaponin XLIV (PS-XLIV) in a human colorectal carcinoma cell line derived from Polygala vulgaris roots.Methods HCT166 cells were treated with different PS-XLIV concentrations and incubated for 24 and 48 h. We used immunocytochemistry to investigate PS-XLIV's anti-cancer properties, employing antibodies targeting WNT3A, WNT11, STAT3, beta-catenin, and Ki-67. The IC50 value of PS-XLIV was 80 mu g/mL in HCT116 cells. WNT11, STAT3, beta-catenin, and Ki-67. Immunoreactivities significantly decreased in PS-XLIV-treated HCT116 cells than in control group cells.Results After PS-XLIV treatment, the epithelial morphology of cells was protected; however, the number of cells was less than that of the control group cells. While WNT3A immunoreactivity was similar in both groups, WNT11 and beta-catenin immunoreactivities were decreased after PS-XLIV application. In addition, the PS-XLIV treated group exhibited significantly weaker Ki-67 immunoreactivity, STAT3 immunoreactivty was moderated after PS-XLIV application.Conclusions For the first time, the anticancer effects of PS-XLIV isolated from P. vulgaris on HCT116 cells were shown. The anticancer effect may involve PS-XLIV reducing WNT11, beta-catenin, STAT3, and Ki-67 activation pathways in HCT116 cells.