Browsing by Author "İçhedef Ç."

Now showing 1 - 4 of 4
  • No Thumbnail Available
    Item
    One-step conjugation of glycylglycine with [18F]FDG and a pilot PET imaging study
    (Springer Netherlands, 2018) Şenk A.M.; İçhedef Ç.; Kılçar A.Y.; Uçar E.; Arı K.; Göksoy D.; Parlak Y.; Sayıt Bilgin B.E.; Teksöz S.
    This study describes a single step conjugation of Glycylglycine (GlyGly) which is a small peptide, with [18F]FDG via oxime formation. Amiooxy-functionalization of GlyGly (AO-GlyGly) was accomplished through the reaction of Boc-aminooxy succinimide ester. Conjugation reaction was performed at 100 °C for 30 min in a vial containing AO-GlyGly and [18F]FDG solution. The radiolabeled product ([18F]FDG-GlyGly) was obtained with 98.65 ± 0.35% yield without any purification step which makes this method more attractive for 18F radiolabeling. The present study is concluded with an in vivo pilot animal PET study to assess biodistribution and kinetics of chemoselectively [18F]FDG tagged GlyGly in vivo. © 2018, Akadémiai Kiadó, Budapest, Hungary.
  • No Thumbnail Available
    Item
    Evaluation of new 99mTc(CO)3 + radiolabeled glycylglycine In Vivo
    (Bentham Science Publishers, 2019) Şenışık A.M.; İçhedef Ç.; Kılçar A.Y.; Uçar E.; Arı K.; Parlak Y.; Bilgin E.S.; Teksöz S.
    Background: Peptide-based agents are used in molecular imaging due to their unique properties, such as rapid clearance from the circulation, high affinity and target selectivity. Many of the radiolabeled peptides have been clinically experienced with diagnostic accuracy. The aim of this study was to investigate in vivo biological behavior of [99mTc(CO)3(H2O)3]+ radiolabeled glycylglycine (GlyGly). Methods: Glycylglycine was radiolabeled with a high radiolabeling yield of 94.69±2%, and quality control of the radiolabeling process was performed by thin layer radiochromatography (TLRC) and High-Performance Liquid Radiochromatography (HPLRC). Lipophilicity study for radiolabeled complex (99mTc(CO)3-Gly-Gly) was carried out using solvent extraction. The in vivo evaluation was performed by both biodistribution and SPECT imaging. Results: The high radiolabelling yield of 99mTc(CO)3-GlyGly was obtained and verified by TLRC and HPLRC as well. According to the in vivo results, SPECT images and biodistribution data are in good accordance. The excretion route from the body was both hepatobiliary and renal. Conclusion: This study shows that 99mTc(CO)3-GlyGly has the potential to be used as a peptide-based imaging agent. Further studies, 99mTc(CO)3-GlyGly can be performed on tumor-bearing animals. © 2019 Bentham Science Publishers.
  • No Thumbnail Available
    Item
    Design of 99mTc radiolabeled gemcitabine polymeric nanoparticles as drug delivery system and in vivo evaluation
    (Elsevier Ltd, 2021) İçhedef Ç.; Teksöz S.; Çetin O.; Aydın B.; Sarıkavak İ.; Parlak Y.; Bilgin B.E.
    The main objective of this study is to create a multifunctional drug carrier system as a prototype product. For this purpose, Gemcitabine that used as an anticancer agent for several kinds of tumour therapy, and technetium(I)-tricarbonyl core, [99mTc(CO)3]+, was incorporated to the polymeric structure. In the current study PLA-PEG-COOH (polylactic acid-polyethylene glycol-carboxyl) copolymer was synthesized and the structure analysis was performed by FT-IR and 1H NMR. The surface charge, size and morphology of the polymeric nanoparticles was evaluated by Zeta Potential (ZP) analysis, Dynamic Light Scattering (DLS) and Scanning Electron Microscopy (SEM) methods. Gemcitabine loaded polymeric nanoparticles were radiolabeled with [99mTc(CO)3(H2O)3]+ and quality controls were performed by thin layer radiochromatograpy (TLRC) and high performance liquid radiochromatograpy (HPLRC). PLA-PEG-GEM complex was labeled with [99mTc(CO)3]+ in high radiochemical yield and purity. The radiolabeled complex is stable during the study period in saline solution. The biological activity of this radiolabeled system was evaluated performing biodistribution and gamma camera imaging on Wistar Albino rats which exhibited higher uptake in the lung and liver at all selected time points. © 2021 Elsevier B.V.
  • No Thumbnail Available
    Item
    Synthesis, radiolabeling and in vitro evaluation of azathioprine loaded magnetic solid lipid nanoparticles
    (Springer Science and Business Media B.V., 2023) Türkkanı G.; Güngör B.; Cetin O.; İçhedef Ç.; Parlak Y.; Gümüşer F.G.; Sayıt Bilgin B.E.; Teksöz S.
    In this study, it is aimed to design a smart drug delivery system for the diagnosis and treatment of cancer cells. Magnetic-solid lipid nanoparticles were synthesized as a drug delivery system and azathioprine (AZA) was loaded into this system. This newly created system was radiolabeled using technetium-99 m with a radiolabeling yield of 90.3%. Drug loading into the SLMNP was found as 86%. The biological behavior of 99mTc and radiolabeled magnetic solid lipid nanoparticles loaded with azathioprine (99mTc-AZA-SLMNP) was investigated in vitro and in vivo. Graphical abstract: [Figure not available: see fulltext.] © 2023, Akadémiai Kiadó, Budapest, Hungary.