Browsing by Author "Şik L."
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Item Genotoxic effects of industrial wastewater on Allium cepa L.(2009) Şik L.; Acar O.; Aki C.The aim of this research is to study the effects of different concentrations of water on both incoming and outgoing in central biological and chemical wastewater treatment plant in Manisa (Turkey) organized industrial zone (MOIZ) on the Allium cepa L. root meristems, having been rooted in distilled water for 48 h. The union bulbs were kept in the 100% concentrations of the refined water (RW) and of 10, 25, 50 and 100% concentrations of unrefined water (UW). Distilled water was used for the control samples. It was determined that wastewater reduced the rate of the mitotic division of different concentrations and increased the mitotic anomalies. Mitotic index was found to be 33.8, 31.2, 23.6 and 16.7% in the control group, RW, 10% concentration of the UW, and 25% concentration of the UW, respectively. On the other hand, the rates of Mitosis / (Anaphase + Telophase) were 0.23, 0.28, 0.42, 0.71 in the control group, RW, % concentration of the UW, and 25% concentration of the UW, respectively. Plant growth was interrupted in the 50 and 100% concentrations of the UW and the mitotic division was inhibited. No anomalies were encountered in the control group. In the RW, a low rate of anomaly was observed, while in the different concentrations of the UW, chromosomal aberrations such as high frequency of lagging chromosome, irregular distribution, polar slips, horizontal division and sticky chromosome were observed. © 2009 Academic Journals.Item Ecological properties of some Crocus taxa in Turkey(2009) Şik L.; Candan F.In this study, ecological properties of the areas where some Crocus taxa (C. ancyrensis, C. sieheanus, C. chrysanthus, C. flavus subsp. flavus, and C. flavus subsp. dissectus) grow naturally was considered. The plants together with in situ soil samples were collected in their flowering periods and data on the distribution areas of the plants and some physical and chemical properties of soil samples were determined. In conclusion, the properties of the new distribution areas for the taxa have been presented and it has been observed that taxa usually grow in light alkali, non-salted, clayey-loamy soils. © 2009 Academic Journals.Item Chromosome numbers of two Colchicum L. species, C. burttii and C. balansae, from Turkey(2009) Şik L.; Kesercioǧlu T.; Candan F.Chromosome numbers and morphologies for Colchicum burttii Meikle and Colchicum balansae Planchon showing distribution in western Anatolia were studied. Squashing preparation method was used. The chromosome counting and morphologies of the species were determined by examining the mitosis prepared from root tips of plants. Chromosome number of C. burttii is 2n = 60 + 2B, showing that this species is hexaploid (x = 10). On the other hand, chromosome number of C. balansae is found as 2n = 90. Formerly, chromosome number of this species was reported as 2n = 32, 54 and 108. In conclusion, chromosome number of the sample obtained with this investigation is different from previous studies. 2n = 90 chromosome number is a basic polyploid (hexaploid) with x2 = 15 (15*6 = 90), involving the summation of the primary basic numbers, 7 and 8. © 2009 Academic Journals.Item Cytotaxonomical studies on some Crocus L. taxa in Turkey(2009) Candan F.; Şik L.; Kesercioǧlu T.The present study investigated the chromosome number and morphology of Crocus fleischeri Gay, C. pallasii Goldb subsp. pallasii, C. cancellatus Herbert subsp. lycius Mathew, C. pulchellus Herbert which are all belong to the Crocus L. taxa showing distribution in Western Anatolia in Turkey. Powder preparation was used as study method. Chromosome numbers and morphologies of the taxa were determined by examining the mitosis preparations. Furthermore, karyograms of taxa studied were made and examined in a detailed manner. In this study, it was found that the chromosome numbers of C. fleischeri, C. pallasii subsp. pallasii, C. cancellatus subsp. lycius and C. pulchellus were 2n = 20, 2n = 14, 2n = 16 and 2n = 12, respectively. © 2009 Academic Journals.Item Micromorphological investigations on pollen samples of four yellow flowered taxa of Crocus L. (Iridaceae) from Turkey(2009) Candan F.; Kesercioǧlu T.; Şik L.In this study, yellow flowered taxa of C. ancyrensis (Herbert) Maw, C. sieheanus Barr ex Burtt, C. flavus Weston subsp. flavus and C. flavus subsp. dissectus T. Baytop & Mathew were examined in the point of pollen micromorphology. Accordingly, it was aimed that pollen micromorphological characteristics of the plant samples could be used as a taxonomic criterion considerably or not. In respect to that purpose, exine structure of pollens as regards samples was examined by scanning electron microscope (SEM). On the other hand, differences between taxa were evaluated. As a result of that investigation, pollen exine ornamentation related to studied four taxa of Crocus L. genus were observed different to some extent and could be used as vice for taxonomic characteristics.Item Crocus antalyensis subsp. striatus subsp. nov. (Iridaceae) from southwest Anatolia(2010) Erol O.; Kocyigit M.; Şik L.; Özhatay N.; Kucuker O.A new subspecies Crocus antalyensis subsp. striatus subsp. nov. from western Turkey is described. It is characterized by rough papery, light brown corm tunics, leaves that exceed the flower at anthesis and are recurved, distinctly striped inner perianth segments and deeply branched pale yellow or white style. The karyotype of the new subspecies is presented. © The Authors. Journal compilation © Nordic Journal of Botany 2010.Item Genetic diversity of Crocus antalyensis B. Mathew (Iridaceae) and a new subspecies from southern Anatolia(2011) Erol O.; Şik L.; Kaya H.B.; Tanyolaç B.; Küçüker O.Crocus antalyensis B. Mathew is a bulbous plant endemic to Turkey. It is morphologically variable within the western part of Anatolia. Amplified fragment length polymorphism (AFLP) marker system was used to detect genetic variation among the Crocus taxa. Twenty-two primer combinations were used to screen for polymorphism among the samples. Genetic variation ranged from 0. 44 to 0. 69. We demonstrated the efficiency of the AFLP marker system for discriminating between individual C. antalyensis specimens. A high level of genetic variation was present among C. antalyensis specimens collected from different locations in Turkey. We also observed that C. antalyensis subspp. are genetically distinct from their relative Crocus flavus Haw. subsp. dissectus Baytop & B. Mathew. A new subspecies of C. antalyensis B. Mathew from southern Turkey is described. It is characterized by striped outer perianth segments, waist-shaped flowers, and glabrous throat of the perianth. A composite image of the new subspecies is presented. © 2011 Springer-Verlag.Item Crocus demirizianus sp. nov. from northwestern Turkey(2012) Erol O.; Can L.; Şik L.A new species, Crocus demirizianus O. Erol & L. Can is described and illustrated from northwestern Turkey. Crocus demirizianus was found in the Çanakkale province, near the district of Çan, and is undoubtedly related to the Crocus biflorus complex. However, the species differs from its relatives by typically sagittate anthers, and flowers without prominent stripes on outer segments. Diagnostic morphological characters are discussed and high resolution images are presented. © 2012 The Authors. Nordic Journal of Botany.Item Te genus Crocus, series Crocus (Iridaceae) in Turkey and 2 East Aegean Islands: A genetic approach(2014) Erol O.; Kaya H.B.; Şik L.; Tuna M.; Can L.; Tanyolaç M.B.In this study, a total of 26 Crocus specimens from different locations across Turkey and 2 East Aegean islands (Chios and Samos) were analyzed using 12 amplified fragment length polymorphism (AFLP) primer combinations to obtain information on genetic diversity, population structure, and genetic relationships. A total of 369 polymorphic AFLP bands were generated and scored as binary data. Genetic similarities were determined. Cluster analysis revealed 4 major groups among the 26 genotypes examined in this study. The nuclear DNA contents (2C) of the 26 Crocus specimens were found to range from 5.08 pg in C. asumaniae to 9.75 pg in C. sativus. Polymorphic information content (PIC) values were used to examine the capacity of the various primer pairs to amplify polymorphisms in the Crocus specimens. The PIC values ranged from 0.218 (M-CAA/E-AGC) to 0.512 (M-CAT/E-AAG) and showed an average of 0.34. In sum, we herein used AFLP analysis to identify a high level of polymorphism among Crocus specimens collected from various locations in Turkey and Greece, and our structural analysis yielded 2 reconstructed populations. Tese fndings provide new insight into the relationships among different Crocus genotypes and show that AFLP analysis can be useful for Crocus diversity studies. © TÜBİTAK.Item Galium shinasii (Rubiaceae): A new species of Galium L. from Eastern Turkey(Pensoft Publishers, 2016) Şik L.; Yildirim H.; Pirhan A.F.; Altioglu Y.; Gemici M.Galium shinasii Yildirim (Rubiaceae), is described as a new species from Malatya Province in eastern Anatolia, Turkey. The new species is morphologically related to Galium cornigerum Boiss. & Hausskn. G. lasiocarpum and G. sorgereae Ehrend. and Schönb. but clearly differs from them based on the morphological differences presented in the species description. In addition, the conservation status, the distribution map, and notes on the biogeography and ecology of the new species are given. © Levent Şik et al.Item Seed structure of some taxa of the genus Crocus L. (iridaceae) series crocus(Turkiye Klinikleri Journal of Medical Sciences, 2018) Karaismailoğlu M.C.; Şik L.; Çiftçi A.; Erol O.In this study, the macro-and micromorphological seed characteristics of the genus Crocus L. series Crocus taxa, including C. pallasii, C. turcicus, C. dispathaceus, C. asumaniae, C. mathewii, C. thomasii, C. oreocreticus, and C. cartwrightianus, were observed. Morphological characteristics, such as the size, shape, color, and seed surface structures, were analyzed using stereo and scanning electron microscopes. Testa layer structures including the outer and inner testae, phytomelan layer structure and thickness, and seed endosperm cell features were studied. Differences in the seed, raphe size, seed surface patterns, inner testa features, and phytomelan layer thickness were observed at an interspecific level. The results revealed differences in the seed morphology and anatomy with a few exceptions. © TÜBİTAK.Item Towards a better understanding of Prunus (Rosaceae): molecular and morphological notes on Prunus subgenus Cerasus in Turkey(Oxford University Press, 2023) Ciftci A.; Gercek Y.C.; Mollman R.; Bostanci Ordu P.I.; Yazici C.; Yaprak A.E.; Morgil H.; Şik L.; Erol O.In this study, we explore the relationships among taxa of Prunus subgenus Cerasus in Turkey using a combination of macromorphology, micromorphology and molecular techniques. We recorded qualitative and quantitative characteristics of flowers, leaves, seeds and endocarp for 96 populations representing 14 taxa of Prunus subgenus Cerasus. ITS, matK, ycf1, trnL-trnF DNA sequence data were used to reconstruct phylogenetic trees for this group. Haplotype analyses results found 11 haplotypes among the samples used in this study. We also note that the infraspecific taxa of Cerasus angustifolia (=P. albicaulis), P. mahaleb, P. microcarpa and P. prostrata, as described by previous authors, do not exhibit significant differences even among the features of foliar morphology that are so often used to distinguish them from one another. As a general rule, our analyses indicate that the highly variable features of leaf micromorphology and endocarp and seed micromorphology are not taxonomically significant. In contrast, although floral morphology is often overlooked in systematic studies of Prunus subgenus Cerasus, we found several taxonomically useful flower characters, including sepal indumentum, hypanthium shape and indumentum and petal shape. This work clarifies the infraspecific relationships of members of Prunus subgenus Cerasus in Turkey and solves some taxonomic problems. © 2023 The Author(s).Item A new autumn-flowering Crocus (Iridaceae) from Türkiye: C. rifatozdemiri sp. nov.(Magnolia Press, 2024) Şik L.; Yazici C.; Mollman R.R.; Harpke D.; Kaleli B.S.; Çiftçi A.; Erol O.C. rifatozdemiri sp. nov. is a new autumn-blooming species from Crocus series Crocus described from three locations on Mount Çal in Muğla province in southwest Türkiye. The new species is notable for its large showy white flowers with sharply mucronate segment tips and contrasting bright red stigma branches. The exceptionally tall stigma is what most clearly differentiates the new species from its closest relatives, with its long branches that usually divide from above the anther tips, although other morphological features, such as tunic neck and tepal shape, as well as molecular data, support its separation from other members of series Crocus. nrETS and nrITS nuclear regions were used to infer the phylogenetic affiliation of the new species within series Crocus, resulting in a clear separation between it and closest relative C. kofudagensis, as well as other members of the series. Leaf anatomy was found to be similar to other members of series Crocus, which tend to show minimal differences between species. Finally, a full description and diagnosis, chromosome count, line illustration and figures detailing the features of this new endemic taxon, which is so far known only from one mountain in Türkiye. Copyright © 2024 Magnolia Press.