Browsing by Author "Akbulut, Z"

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    LC-MS/MS analysis, antioxidant and anticancer effects of phenolic-rich extracts from Algerian propolis: a comparative study
    Boulechfar, S; Akbulut, Z; Tepe, HD; Zellagui, A; Aktas, RG; Bensouici, C; Doyuk, F; Khattabi, L; Demirel, G; Lahouel, M
    The present study was aimed to compare the phenolic composition and the functional properties (antioxidant and anticancer) of phenolic-rich extracts of two different propolis collected from Collo (PREPC) and Oum El Bouaghi (PREPO) regions. The phenolic composition was determined using LC/MS-MS. The antioxidant activity of the extracts was evaluated using alkaline DMSO and beta-carotene-linoleic acid tests. Anticancer effect on HepG2 human hepatocellular carcinoma cells was determined using CCK-8 assay. Twenty-two phenolic compounds in PREPC and twenty-three in PREPO were detected and quantified by LC-MS/MS. Ferulic and caffeic acids were found to be the predominant compounds. Both extracts were able to inhibit lipid peroxidation and demonstrated their ability to scavenge superoxide radicals more effectively than the standards. The highest lipid peroxidation inhibition (IC50 = 17.58 +/- 1.98 mu g/mL) and superoxide radical scavenging effects (IC50 = 6.19 +/- 0.24 mu g/mL) were exhibited by PREPO. However, PREPC showed stronger cytotoxic activity against HepG2 (IC50 = 12.22 +/- 0.05 mu g/mL) than PREPO (IC50 = 18.68 +/- 0.33 mu g/mL). These results demonstrate the potential of extracts from Algerian propolis to be used in functional formulations.
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    A Retrospective Analysis of 83 Patients with Testicular Mass Who Underwent Testis-Sparing Surgery: The Eurasian Uro-oncology Association Multicenter Study
    Keske, M; Canda, AE; Karadag, MA; Çiftçi, H; Erturhan, S; Kactan, C; Soytas, M; Özkaya, F; Ozbey, I; Ordek, E; Atmaca, AF; Yildirim, A; Sahin, S; Colakoglu, Y; Boylu, U; Erol, B; Caskurlu, T; Kiremit, MC; Cakici, OU; Sonmez, G; Kiliçarslan, H; Akbulut, Z; Kaygisiz, O; Bedir, S; Vuruskan, H; Bozkurt, YE; Aydin, HR; Oguz, U; Basok, EK; Gumus, BH; Tuncel, A; Aslan, Y; Hamidi, N; Müslümanoglu, AY; Dinçer, M; Balbay, D; Albayrak, S; Laguna, MP
    Introduction: Herein, we analyzed the histopathological, oncological and functional outcomes of testis-sparing surgery (TSS) in patients with distinct risk for testicular cancer. Methods: This is a multicenter retrospective study on consecutive patients who underwent TSS. Patients were categorized in high- or low-risk testicular germ cell tumor (TGCT) according to the presence/absence of features compatible with testicular dysgenesis syndrome. Histology was categorized per size and risk groups. Results: TSS was performed in 83 patients (86 tumors) of them, 27 in the high-risk group. Fifty-nine patients had a non-tumoral contralateral testis present. Sixty masses and 26 masses were benign and TGCTs, respectively. No statistical differences were observed in mean age (30.9 +/- 10.32 years), pathological tumor size (14.67 +/- 6.7 mm) between risk groups or between benign and malignant tumors (p = 0.608). When categorized per risk groups, 22 (73.3%) and 4 (7.1%) of the TSS specimens were malignant in the high- and low-risk patient groups, respectively. Univariate analysis showed that the only independent variable significantly related to malignant outcome was previous history of TGCT. During a mean follow-up of 25.5 +/- 22.7 months, no patient developed systemic disease. Local recurrence was detected in 5 patients and received radical orchiectomy. Postoperative testosterone levels remained normal in 88% of those patients with normal preoperative level. No erectile dysfunction was reported in patients with benign lesions. Conclusion: TSS is a safe and feasible approach with adequate cancer control, and preservation of sexual function is possible in 2/3 of patients harboring malignancy. Incidence of TGCT varies extremely between patients at high and low risk for TGCT requiring a careful consideration and counseling.