Browsing by Author "Akduman, E"

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    DISTRIBUTION OF HEPATITIS C VIRUS GENOTYPES IN MANISA REGION, TURKEY
    Sanlidag, T; Akçali, S; Özbakkaloglu, B; Ertekin, D; Akduman, E
    The duration of hepatitis C virus (HCV) infection and the response to the standard therapy is strongly related to the HCV genotypes. In addition, the geographical distribution of HCV genotypes is important for the epidemiological studies in terms of distribution and possible risk groups. The aim of this retrospective study was to investigate the distribution of HCV genotypes in Manisa region (located at the Aegean part of Turkey). A total of 100 anti-HCV (microparticle EIA; Abbott Laboratories, USA) and HCV-RNA (real time RT-PCR; Applied Biosystems, USA) positive patients (53 female, 47 male; mean age: 44.4 +/- 10.4 years), who were admitted to Celal Bayar University Medical School Hospital between 2002-2005, were included to the study. Quantitative HCV-RNA levels of the patients were between 10(4)-10(8) copies/ml. Complementary DNAs obtained from HCV-RNAs isolated by Invitek RTP DNA/RNA Virus Mini Kit were used for genotyping with selected primers [primer 11 (5'-AGG TCT CTG AGA CCG TGC ACC ATG AGC AC-3') and primer 13 (5'-CTG TGA GGA ACT ACT GTC TT-3') for the first PCR; primer 12 (5'-ACT GCC TGA TAG GGT GCT TGC GAG TG-3') and primer 14 (5'-CAC GCA GAA AGC GTC TAG-3') for the second PCR]. The RT-PCR products were purified with Invisorb Spin PCRapid Kit and sequenced by BigDye Terminator v3.1 Cycle Sequencing Kit in ABI Prism 310 Genetic Analyzer. Genotype I was found in 92% of the patients (92%) and genotypes 2 and 4 were found in 7% of the patients, while HCV genotype could not be identified in one patient (1%). When evaluating the subtypes, genotype 1b was determined in 90 patients (90%), genotype 4a in five patients (5%), genotype 1 a in two patients (2%) and genotype 2a in two patients (2%). In conclusion, 1b was found to be the most common HCV genotype in Manisa region in concordance with the previous data obtained in Turkey, followed by genotype 4a, although a rare one. The data of this study is noteworthy especially for the arrangement of treatment and follow-up of HCV infected patients.
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    A novel concept: occult HBV infection in the western region of Turkey
    Akduman, E; Sanlidag, T; Akcali, S; Ellidokuz, E; Celebi Kobak, A
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    The Comparison of Cell Culture, Hybridization and Direct Flourescent Antibody Tests in Chlamydia trachomatis Diagnosis
    Akduman, E; Ecemis, T; Sagol, S; Çiçek, C; Vatansever, S; Özbakkaloglu, B
    Objective: The aim of this study was to compare the reliability of hybridization and direct fluorescent antibody tests for the diagnosis of genital Chlamydia trachomatis infections. Material and Methods: Cervical specimens from 100 infertile women were evaluated with hybridizasyon, direct fluorescent antibody tests, and cell culture method known as a gold standard, and also sensitivity, specificity, positive and negative predictive values of the three methods were compared. Results: C. trachomatis was isolated in cell cultures of 11 samples. Seven and 19 positive samples were found respectively through direct fluorescent antibody, and hybridization tests identified 7, and 19 positive samples, respectively. Direct fluorescent antibody tests demonstrated 54.5 % sensitivity, 98 % specificity, 47.4 % positive, and 94.6 % negative predictive value, while the corresponding percentages for hybridization tests were 81.8, 88.8, 47.4, and 97.5 %, respectively. Conclusion: It was concluded that a single method has not sufficient sensitivity and specificity for the establishment of a definitive diagnosis C. trachomatis infection, and the results should be confirmed by other methods the hybridization method being more rapid and effective than the other methods.