Browsing by Author "Akduman B."

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    A survey of patient preparation and technique of ultrasound-guided prostate biopsy: A multicenter study og urooncological association; [Ultrasonografi kιlavuzluǧunda yapιlan prostat biyopsisinde hasta hazιrlιǧι ve teknik anketi: Üroonkoloji derneǧi çcok merkezli çalιşmasι]
    (Turk Uroloji Dernegi, 2007) Bozlu M.; Akduman B.; Mungan U.; Özen H.; Baltaci S.; Türkeri L.; Kirkali Z.; Akdaş A.; Adsan Ö.; Akdoǧan B.; Altinel M.; Ataus S.; Ayan S.; Bilen C.Y.; Çal Ç.; Çek M.; Dündar M.; Işeri C.; Koşan M.; Lekili M.; Müezzinoǧlu T.; Özer G.; Özgök Y.; Perk H.; Soyupak B.; Soyupek S.; Soylu A.; Sözen S.; Şengör F.; Tansuǧ Z.; Tekin A.; Yildirim A.
    Introduction: Ultrasound-guided prostate biopsy is the standard method for the diagnosis of prostate cancer. The aim of the present survey is to assess the variability in patient preparation and technique of ultrasound-guided prostate biopsy among Turkish Urologists. Materials and Methods: In July 2004, a questionnaire was sent out to e-mail addresses of the members of Urooncological Association, asking about the details of prostate biopsy protocol of the members. The survey consisted of multiple choice questions about the patient preparation and prostate biopsy technique. Responses were acquired via e-mail and analyzed in detail. Results: Thirty two urologists from 24 centers responded. The biopsy procedure was performed by the urologist only in 54.16% of the centers, both urologist and radiologist in 37.5%, and radiologist only in 8.33%. Transrectal route was the most common method for ultrasound-guided prostate biopsy. A half of the responders performed biopsy when PSA was greater than 4 ng/ml. All of the centers administered antibiotic and a half of them used enema before the procedure. Approximately 37% of responders did not administer any type of analgesia, but 29.1% of all responders administered a periprostatic nerve block for reducing pain during the procedure. Most urologists obtained 10 or 12 biopsy cores and only 20.8% of them obtained routine transitional zone biopsy during the initial biopsy session. Conclusion: This survey demonstrated that patient preparation and technique of ultrasound-guided prostate biopsy is not standardized among Turkish Urologists, and a guideline on prostate biopsy is needed.
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    Immobilization of amyloglucosidase onto macroporous cryogels for continuous glucose production from starch
    (Taylor and Francis Inc., 2015) Uygun M.; Akduman B.; Ergönül B.; Aktaş Uygun D.; Akgöl S.; Denizli A.
    Poly(methyl methacrylate-glycidyl methacrylate) [Poly(MMA-GMA)] cryogels were synthesized using monomers of methylmethacrylic acid and epoxy group bearing GMA via radical cryopolymerization technique. Synthesized cryogels were used for the immobilization of amyloglucosidase to the cryogel surface using epoxy chemistry. Characterizations of the free and immobilized amyloglucosidase were carried out by comparing the optimum and kinetic parameters of enzymes. For this, pH and temperature profiles of free and immobilized preparation were studied and, it was found that, optimum pH of enzyme was not change upon immobilization (pH 5.0), while optimum temperature of the enzyme shifted 10 °C to warmer region after immobilization (optimum temperatures for free and immobilized enzyme were 55 and 65 °C, respectively). Kinetic parameters of free and immobilized enzyme were also investigated and Km values of free and immobilized amyloglucosidase were found to be 2.743 and 0.865 mg/mL, respectively. Vmax of immobilized amyloglucosidase was found to be (0.496 mol/min) about four times less than that of free enzyme (2.020 mol/min). Storage and operational stabilities of immobilized amyloglucosidase were also studied and it was showed that immobilized preparation had much more stability than free preparation. In the present work, amyloglucosidase immobilized poly(MMA-GMA) cryogels were used for continuous glucose syrup production from starch for the first time. Efficiency of immobilized enzyme was investigated and released amount of glucose was found to be 2.54 mg/mL at the end of the 5 min of hydrolysis. The results indicate that the epoxy functionalized cryogels offer a good alternative for amyloglucosidase immobilization applications with increased operational and thermal stability, and reusability. Also, these cryogels can be used for immobilization of other industrially valuable enzymes beyond amyloglucosidase. © 2015 Taylor & Francis.