Browsing by Author "Aker Y."

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    The effects of sterile or infected bile and dropped gallstones in abdominal adhesions and abscess formation
    (Springer New York, 1997) Soybir G.R.; Köksoy F.; Polat C.; Özşeker A.; Yalçln O.; Aker Y.; Topuzlu C.
    Background: The effects of gallstones and sterile or infected bile on postoperative adhesions and abscess formation were investigated in Sprague Dawley rats. Methods: The first three groups were injected intraperitoneally with serum saline, sterile bile, or infected bile, respectively. Laparotomy was adjusted to the next seven groups. Serum saline, sterile bile, and infected bile were injected in the fourth through sixth groups intraperitoneally, respectively. Gallstones were placed intraabdominally to the seventh through ninth groups. The injections of sterile bile in group 7 and of infected bile in group 8 were added to laparotomies. Only laparotomy was carried out in group 10. Results: Abscess formations were seen only in infected-bile-injected groups. Significant adhesion formations were seen in infected-bile groups. Sterile bile and/or gallstones had no significant effect in abscess or adhesion formation. Conclusions: Results suggest that noninfected gallstones and sterile bile, even in combination, do not increase post-operative intraabdominal complications in rats.
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    Diagnosis of intestinal ischemia by measurement of serum phosphate and enzyme changes and the effectiveness of vitamin E treatment; [Serum fosfor ve enzim degisiklikleri yardimiyla intestinal iskemi tanisinin konmasi ve tedavi icin kullanilan e vitamininin etkinliginin belirlenmesi]
    (1999) Uncu H.; Uncu G.; Ilcol Y.; Aker Y.
    Ischemia of the intestine is associated with acute injury of other organs and early diagnosis and surgical intervention is necessary to reduce morbidity and mortality. The aim of this study was to investigate changes in serum enzymes and phosphate values following diagnosis of intestinal ischemia and to determine the effects of vitamin E on its treatment. Twenty New Zealand rabbits were seperated randomly into two groups. In the first group, superior mesenteric arteries were ligated, blood samples taken and ALT, AST, ALP, LDH, CK and P levels measured after one hour of ischemia. In the second group, intestinal ischemia was induced, vitamin E injected following ten minutes of ischemia and the same biochemical investigations performed after one hour of ischemia. There was no significant difference between the results of the two groups. The laboratory parameters may be of benefit in the early diagnosis of intestinal ischemia. However, the results of these parameters were not changed by the use of vitamin E as an antioxidant.
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    Free oxygen radical-induced acute pancreatitis. A light and electron microscopic study
    (2003) Coskun T.; Korkusuz P.; Kaya Y.; Örs Ü.; Aker Y.; Kilinç K.
    Background/Aims: To date direct toxic effects of free oxygen radicals in vivo on pancreatic parenchyma have not been studied thoroughly. We aimed to study: 1) the detailed histopathological changes induced by free oxygen radicals in pancreas; and 2) the preventive effect of intraductal catalase in H2O2-induced acute pancreatitis. Methodology: Wistar Albino rats were randomized into six groups. 1) First experiment: Bile-pancreatic duct was cannulated close to the liver and perfused through the duodenum with (i) normal saline solution, (ii) iron sulfate (FeSO4), (iii) hydrogen peroxide (H2O2), (iv) hydrogen peroxide and iron sulfate simultaneously. 2) Second experiment: Bile pancreatic duct was perfused either with H2O2 or H2O2+catalase. Serum amylase and pancreas malondialdehyde levels were measured in both experiments after 3 hours of perfusion period. Tissue samples were obtained for histopathological examinations. Results: 1) First experiment: Intraductal perfusion of FeSO4 or H2O2 or H2O2+FeSO4 induced acute edematous pancreatitis with focal parenchymal necrosis. At the ultrastructural level, intracytoplasmic formation of vacuoles, fusion of the vacuoles and zymogen granules, and autophagosomes containing cellular organelles were found. Serum amylase and pancreas malondialdehyde levels, and morphological score were significantly higher in these groups than control group (p<0.001). 2) Second experiment: Catalase perfusion simultaneously with H2O2 decreased the serum amylase and pancreas malondialdehyde levels, and morphological score significantly (p<0.001) and prevented the desquamation of the columnar epithelium and development of gross edema but not parenchymal necrosis. Conclusions: Intraductal perfusion of FeSO4 or H2O2 or H2O2+FeSO4-induced acute pancreatitis with marked light and electronmicroscopic changes. Intraductal perfusion of catalase and H2O2 simultaneously did not prevent or lessen the parenchymal necrosis. These findings have suggested that another mechanism of injury may also play a role in parenchymal injury in oxygen radical-induced acute pancreatitis.