Browsing by Author "Akercan, F"

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    Evaluation Of VEGF, EGF-R AND TGF-α In Placenta Of Pregnancies With Preeclampsia By Immunohistochemistry
    Çirpanli, T; Akercan, F; Terek, MC; Özcakir, HT; Giray, G; Sagol, S; Karadadas, N
    Objective: The aim of the study is to determine VEGF, EGFR and TGF-alpha with the immunohistochemical staining in placenta biopsies of patients with preeclampsia. Design: Prospective case control study. Materials and Methods: Biopsies from the placenta were obtained from ten patients with preeclapmsia and ten patients of control group during the cesarean section. Placenta biopsies were examined for VEGF, EGF-R and TGF-alpha distribution with avidin-biotinperoxidase immunohistochemistry. The staining intensities were graded semi-quantitatively by the two histologists who were blinded to the groups and the H-score was calculated by light microscopic examination. Results: The VEGF, EGFR and TGF-alpha expression was significantly higher in placenta biopsies of preeclamptic patient compared to controls (271.2 +/- 22.65 versus 201.9 +/- 12.33, p=0.000; 186.3 +/- 4.98 versus 150.3 +/- 5.7, p=0.000; 185.1 +/- 7.48 versus 169.2 +/- 6.19, p=0.000; respectively). Conclusion: Immunostaining of VEGF, EGF-R and TGF-alpha is significantly higher in placenta biopsies in patients with preeclampsia.
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    Evaluation of VEGF in placental bed biopsies from preeclamptic women by immunohistochemistry
    Cirpan, T; Akercan, F; Terek, MC; Kazandi, M; Ozcakir, HT; Giray, G; Sagol, S
    Objective: The aim of the study was to determine VEGF protein with immunohistochemical staining in placental bed biopsies of preeclamptic pregnancies in comparison to normal controls. Design: Prospective cohort Study. Methods: The placental bed biopsies were obtained from 12 patients with preeclapmsia and ten patients for a control group at the time of cesarean delivery. Tissue samples of the placental bed were examined for VEGF protein distribution with avidin-biotin-peroxidase immunohistochemistry. Two blinded histopathologists were asked to score each sample for the intensity of staining and the number of cells stained in a randomly selected HPF of each sample. The resulting H-score was computed as a product of intensity and percent of cells stained. Results: VEGF expression was significantly lower in both the myometrium and stroma of the preeclamptic group compared to the control group (77.2 +/- 25.4 vs 134 +/- 44.3, p = 0.007; 194.1 +/- 20.7 vs 170.2 +/- 17, p = 0.017, respectively). Conclusion: VEGF expression is significantly lower in placental bed biopsies of preeclamptic pregnancies.
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    The immunohistochemical evaluation of VEGF in placenta biopsies of pregnancies complicated by preeclampsia
    Akercan, F; Cirpan, T; Terek, MC; Ozcakir, HT; Giray, G; Sagol, S; Karadadas, N
    Objective The study was designed to determine the protein levels of vascular endothelial growth factor (VEGF) in the placenta biopsies of patients with preeclampsia and compare with normal controls. Design Prospective cohort study. Methods The placental biopsies were obtained from ten patients with preeclampsia and ten patients of control group at the time of delivery. Avidin-biotin-peroxidase immunohistochemistry was then performed to identify levels of VEGF protein within the tissue and a semi-quantitative method was devised to score the amount of staining present in the sample. Two histopathologists who were blinded to the groups were asked to score each sample for the intensity of staining and the number of cells stained in a randomly selected per high-power fields of each sample. The resulting H-score was computed as a product of intensity and percent of cells stained. Results The VEGF expression was significantly higher in placenta biopsies of preeclamptic patients compared to that of controls (271.2 +/- 22.65 vs. 201.9 +/- 12.33, P = 0.000). Conclusion Immunostaining of VEGF is significantly higher in placenta biopsies of patients with preeclampsia.
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    Relation between mothers' types of labor, birth interventions, birth experiences and postpartum depression: A multicentre follow-up study
    Atan, SU; Ozturk, R; Satir, DG; Çalim, SI; Weller, BK; Amanak, K; Saruhan, A; Sirin, A; Akercan, F
    Aim: This definitive and cross-sectional study was conducted to determine the relation between mothers' types of labor, birth interventions, birth experiences and postpartum depression. Methods: A total of 1010 mothers who gave birth in four different provinces of Turkey were chosen to participate in the study via purposive sampling method Results: The Edinburgh Postpartum Depression Scale score was determined to be 13 and over in 36.4% of the women. In this study, it was determined that the Edinburgh Postpartum Depression Scale scores for women in the 18-24 age group who had a vaginal birth, did not have health insurance, experienced health problems during pregnancy and were not trained about type of labor during pregnancy were statistically higher. There was no significant correlation between the birth experiences and postpartum depression. The linear regression model showed that there was a statistically significant correlation between enema and amniotomy interventions practised during the birth and the Edinburgh Postpartum Depression Scale scores. Conclusion: In conclusion, it is thought that preparing the mothers for birth with birth preparation training in the antenatal period and imposing the necessary regulations in the delivery room for the mothers to have a positive birth experience are important in reducing postpartum depression risk.