Browsing by Author "Aslan, M"

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    Detection of anti-leishmanial effect of the Lucilia sericata larval secretions in vitro and in vivo on Leishmania tropica: First work
    Polat, E; Cakan, H; Aslan, M; Sirekbasan, S; Kutlubay, Z; Ipek, T; Ozbilgin, A
    It is known that some of the enzymes and substances secreted by 2nd and 3rd stages of the Lucilia sericata larvae to have bacteriostatic and bactericidal effects. From this point of view, we investigated the anti-leishmanial effect of larval secretions of the L. sericata on the Leishmania tropica both in vitro and in vivo conditions. In vitro: It was observed that promastigotes of L. tropica had undergone lyzis within 1 min in the larval secretions of L. sericata. However, larval secretion was ineffective on the promastigotes within Novy-Mac-Neal-Nicolle (NNN) cultures and RPMI 1640 medium. In vivo: Seven groups of male Balb/C mice (6 study groups and 1 control group), each composed of eight weeks old 10 mice were formed. L. tropica promastigotes were injected subcutaneosly to the soles of the SG mice' feet. In study groups, cutaneous lesions were developed Limoncu et al., 1997 in 2 (20%) and 1 (10%) of the SG-1 and SG-2, respectively after 15 days. There were L. tropica in the smears prepared from the lesions and L tropica was observed in the cultures. Cutaneous lesions were not developed in 8 (80%), 9 (90%) and 10(100%) of the SG-I, SG-II and SG-III, respectively. There were no cutaneous lesions developed in the soles of the feet. There were no L. tropica in the smears prepared from the infected soles of the feet neither L. tropica was observed in the cultures. Larval secretions were given into the cutaneous lesions to the feet soles of the SG-IV, V and VI mice after 6 months. No healing was observed in the cutaneos lesions of 4 (40%), 5 (50%) and 1(10%) of SG-IV, SG-V and SG-VI, after 6 months, respectively. There were L tropica in the smears prepared from the lesions and L. tropica was observed in the cultures. On the other hand, the lesions of 6 (60%), 5 and 9(90%) of SG-IV, SG-V and SG-VI were diminished in size and disappeared completely after 6 months. There were L. tropica observed in the smears prepared from the infected soles of the feet and no growth was observed in the cultures. In the smears prepared from the cutaneous lesions developed in the soles of the feet of the control group mice. L. tropica was visualized and observed in the cultures. A statistical significant difference was observed between study groups and control group (p < 0.001). In our study we demonstrated for the first time that the secretions of the 2nd and 3rd stages sterile and pure larvae of L. sericata had effects on promastigotes of L. tropica in in vitro and very effective on amastigote forms in in vivo conditions. (c) 2012 Elsevier Inc. All rights reserved.
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    Breast-feeding knowledge and practices among mothers in Manisa, Turkey
    Yanikkerem, E; Tuncer, R; Yilmaz, K; Aslan, M; Karadeniz, G
    Objective: to determine breast-feeding knowledge and practices among mothers before and after an educational intervention on breast feeding. Design: cross-sectional, descriptive study using a face-to-face questionnaire. Setting: Manisa Maternity and Children's Hospital, which has Baby Friendly status. Participants: 158 women in the early postpartum period. Measurements and findings: 10.8% of women were not aware that they should offer colostrum to their babies. Only 43.7% of women commenced breast feeding with in the first 30 minutes of giving birth. Prior to receiving the educational intervention, 55.7% of mothers knew how often they should feed their baby, 48.7% knew that they should breast feed their baby for two years together with additional food, although 55.1% of women did not know how to hold their breast during a feed. The mean pre-tests core was 9.9 (SD 2.4; range 3-13), and the mean post-tests core was 12.6 (SD 0.8; range 9-13). The difference between the mean pre- and post-test scores was statistically significant (paired t = 15.3, df = 157, P<0.001). Conclusion and implications for practice: given the integral role of breast feeding in improving overall community health, regular breast-feeding counselling should be offered, particularly during the pregnancy and early postpartum period. (C) 2007 Elsevier Ltd. All rights reserved.
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    Serum adenosine deaminase, catalase and carbonic anhydrase activities in patients with bladder cancer
    Pirinççi, N; Geçit, I; Günes, M; Yüksel, MB; Kaba, M; Tanik, S; Demir, H; Aslan, M
    OBJECTIVES: The relationship between adenosine deaminase and various cancers has been investigated in several studies. However, serum adenosine deaminase activity and carbonic anhydrase and catalase activities in patients with bladder cancer have not previously been reported. Therefore, the aim of this study was to measure serum adenosine deaminase, carbonic anhydrase and catalase activities in patients with bladder cancer. MATERIALS AND METHODS: Forty patients with bladder cancer and 30 healthy controls were enrolled in the study. Serum adenosine deaminase, carbonic anhydrase and catalase activities were measured spectrophotometrically. RESULTS: Serum adenosine deaminase, carbonic anhydrase and catalase activities were significantly higher in patients with bladder cancer than controls (all significant, p < 0.001). CONCLUSIONS: These markers might be a potentially important finding as an additional diagnostic biochemical tool for bladder cancer.
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    Haptoglobin Levels in Turkish Patients with Bladder Cancer and its Association with Clinicopathological Features
    Piriniççi, N; Geçit, I; Gunes, M; Kemik, AS; Yüksel, MB; Kaba, M; Ceylan, K; Aslan, M
    Although alteration in the haptoglobin phenotype has been reported in patients with bladder cancer, serum haptoglobin levels have not been evaluated. We hypothesized that serum haptoglobin can be used as a biomarker. The aim of this study was to evaluate the expression of haptoglobin in bladder cancer and to determine the relationship with clinicopathological features. A total of 68 serum specimens obtained before surgery were used to investigate haptoglobin expression using the sandwich ELISA technique. Serum haptoglobin levels were higher in the patients with bladder cancer compared to healthy controls (p<0.0001). Additionally, the levels of haptoglobin protein increased with increasing tumor grades (p<0.001) and were significantly higher in patients with metastatic disease and the presence of lymphovascular involvement, lymph node metastases and increasing tumor burden (p<0.0001). This study suggests that elevated haptoglobin levels are associated with a higher stage, grade, and extent of distant metastasis and larger tumor size. Haptoglobin may therefore provide a useful diagnostic and treatment biomarker for patients with bladder cancer.