Browsing by Author "Aslan H."

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    Efficiency of bioflavonoids in the prevention of experimental myringosclerosis
    (2010) Ilknur A.E.; Dundar R.; Basoglu S.; Inan S.; Aktas S.; Aslan H.; Ozkul Y.; Ozturkcan S.; Katilmis H.
    Objectives/Hypothesis: It has been noted that some materials with anti-inflammatory and antioxidant effects decrease sclerotic lesions in experimental myringosclerosis. Our purpose in this study is to investigate the effect of micronized purified flavonoid fraction (MPFF), an antioxidant with anti-inflammatory effects, in experimental myringosclerosis in guinea pigs. Materials and Methods: Two study groups were formed. The first group was administered 100mg/kg/day MPFF by catheter for five days before myringotomy and 10 days after myringotomy, while the second group was administered distilled water by the same method, before and after myringotomy. On the 15th day of the study, after the tympanic membranes were examined otomicroscopically for myringosclerosis, they were removed by dissection together with the bone annulus, for histochemical and immunohistochemical examinations. Results: In the MPFF group, the otomicroscopical sclerosis score, inflammation score and tympanic membrane thickness were significantly less than those in the untreated group (p<0.05). It was also determined that the immunoactivity of the anti-VEGF, anti-TGF-beta, anti-eNOS, anti-iNOS, and anti-IL1-beta primary antibodies, which are known to have an important role in angiogenesis and inflammation, significantly decreased in the MPFF group (p<0.05). Conclusion: In conclusion, this study shows that orally administered MPFF can be efficient in the prevention of experimental myringosclerosis in guinea pigs. Copyright 2005 © The Mediterranean Society of Otology and Audiology.
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    Increased expression of VEGF, iNOS, IL-1β, and IL-17 in a rabbit model of gastric content-induced middle ear inflammation
    (2012) Başoĝlu M.S.; Eren E.; Aslan H.; Kolatan H.E.; Özbay C.; Ïnan S.; Karaca F.; Öztürkcan S.; Katilmiş H.
    Objective: To investigate the histopathological changes and the expression of vascular endothelial growth factor (VEGF), inducible NO-synthase (iNOS), endothelial NO-synthase (eNOS), interleukin (IL)-1β, and IL-17 in the rabbit middle ear mucosa after direct gastric content exposure. Methods: Exploratory controlled study in which histological and immunochemical features were studied after gastric content-induced inflammation was established in rabbits. Sixteen healthy rabbits were divided into two equal groups. Gastric contents of an animal were injected into the middle ear of the same animal for 20 days. Saline was injected into the middle ear of the animals in the control group. The rabbits were humanely killed on day 27. Inflammation was assayed by light microscopy. Immunochemical staining was performed for VEGF, iNOS, eNOS, IL-1β, and IL-17 expression. Experimental and control animals were examined using the same protocol. Results: The expression levels of VEGF, iNOS, IL-1β, and IL-17 differed significantly between the experimental and control groups (p=0.018, p=0.010, p=0.002, and p=0.002, respectively). The expression level of eNOS was not significantly different between the two groups (p=0.132). Conclusion: This study demonstrates that gastroesophagial reflux induced middle ear inflammation is associated with increased expression of VEGF, IL-1β, IL-17, and iNOS. © 2011 Elsevier Ireland Ltd.