Browsing by Author "Atmane, SA"

Now showing 1 - 3 of 3
  • No Thumbnail Available
    Item
    Valorization of Pinus halepensis Mill. seed oil: Physicochemical characteristics, bioactive compounds, and antioxidant activity as affected by location and extraction method
    Atmane, SA; Özbek, ZA; Ergönül, PG; Khettal, B
    In this study, physicochemical characteristics, phytochemical compositions, and antioxidant activities of Pinus halepensis Mill. seed oils (PHSO) from two geographical origins (Algeria and Turkey) and obtained through two different extraction processes (cold pressing and solvent extraction) were determined. PHSO were majorly consisted of linoleic and oleic acids. Cold pressing yielded oils with lower peroxide and totox values, higher tocopherols contents (309.42-318.04 mg/100 g), higher DPPH-radical scavenging activities (25.33-26.94 mM TE/kg), and reducing capacity values (301.30-329.94 mg GAE/kg). The solvent-extracted oils contained more phytosterols (5,683.25-5,964.20 mg/kg), particularly beta-sitosterol and campesterol. Phenolic compounds of PHSO were identified by HPLC-DAD for the first time in this study. More phenolic compounds were detected in solvent-extracted oils. Turkish PHSO were richer in individual phenolics (18,928.92 mu g/100 g) than the Algerian variety (2,821.01 mu g/100 g). Different extraction techniques and geographical origins led to differences in physicochemical and phytochemical attributes of PHSO. Novelty impact statement This study revealed that extraction technique (cold-pressing, solvent extraction) and geographical seed origin (Algeria, Turkey) influenced the Pinus halepensis Mill. seed oils (PHSO) composition. PHSO are rich in fatty acids, sterols, and tocopherols majorly linoleic acid, gamma-tocopherol, and beta-sitosterol which were detected for first time for the cold pressed oils. This is the first report on phenolics compounds of these oils, mainly luteolin. The high oxidative stability and good antioxidant activities of PHSO promote their exploitation in food, cosmetics, and pharmaceuticals uses.
  • No Thumbnail Available
    Item
    Acute and 28-day repeated dose toxicity evaluations of cold pressed Pinus halepensis Mill. seed oil in mice and rats
    Atmane, SA; Eldjoudi, DA; Özbek, ZA; Ergönül, PG; Khettal, B
    Pinus halepensis Mill. seed (Pinaceae), popularly known as `Zgougou', is widely consumed in the Mediterranean countries and used traditionally in the treatment of some diseases such as bronchitis, rheumatism, infection, and inflammation. The present study aimed to evaluate the oral safety of cold pressed oil of Pinus halepensis Mill. seeds (COPHS) by acute and 28-day repeated dose toxicities studies in Wistar mice and rats, respectively. In the acute toxicity study, oral administration of COPHS to mice did not provoke mortality or any toxic signs at doses up to 5000 mg/kg bw. After administration of COPHS at doses of 250, 500, and 1000 mg/kg bw/day for 28 days, no abnormal changes were observed in body weight, water intake, food consumption, organ weight, blood haematological, serum biochemistry parameters, and histology profile. Furthermore, there was no animal death or any symptom of toxicity in any group during sub-acute toxicity test period. Our findings demonstrate that COPHS is relatively non-toxic and has a large safety margin (>5000 mg/kg). The results of the present research provide basic reference data for food consumption and for future in vivo screening of biological and pharmacological properties of cold pressed oil of Pinus halepensis Mill. seeds.
  • No Thumbnail Available
    Item
    Cold pressed Pinus halepensis Mill. seed oil for potential health applications: Analgesic, anti-inflammatory effects, and assessment of inflammatory mediators by RT-qPCR in skin wound healing
    Atmane, SA; Batir, MB; Özbek, ZA; Ergönül, PG; Balcan, E; Eldjoudi, DA; Özkale, E; Bribi, N; Khettal, B
    Ethnopharmacological relevance: Pinus halepensis Mill. seed (Pinaceae), commonly known as Zgougou, is widely consumed in the Mediterranean region and has long been used to treat diseases including bronchitis, rheumatism, infection, and inflammation.Aim of the study: The present study was focused on the investigation of some pharmacological activities, such as analgesic, anti-inflammatory, and wound healing effects, of cold-pressed oil of Pinus halepensis Mill. seed (COPHS).Materials and methods: The fixed oil of Pinus halepensis seed was extracted by a cold pressing process. The analgesic activity of COPHS was examined by acetic acid and formalin-induced contortion and pain tests. The anti-inflammatory effects were evaluated in carrageenan and formalin induced paw and ear edema models. Wound healing potential was assessed on an experimental skin wound, and the expression levels of inflammation mediators were determined by RT-qPCR in skin wound healing. Results: The results showed analgesic efficacy through significant inhibition of abdominal cramps (59.15%) and pain (75.91%). In addition, this oil exerted an anti-inflammatory effect by inhibiting ear (62.25%) and paw (70.00%) edema. The COPHS stimulated wound contraction in experimental skin wound healing with a contraction rate of 89.23% with notable reduction of TNF-alpha and NF-kB expression levels in the treated groups. Conclusions: This study provided for the first time the pharmacological profile, particularly the analgesic, antiinflammatory, and healing effects, of fixed oil extracted by cold pressing from the seed of Pinus halepensis Mill.