Browsing by Author "AvcIbaşI U."

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    Isotopic dilution analysis of zinc in some tissue samples of urogenital systems of rats using 65Zn
    (2009) Ünak T.; Çal Ç.; Yildirim Y.; AvcIbaşI U.; Ünak G.
    Zinc plays an important role in prostate functions. For this reason, in this study some rats have been nourished with foods containing different zinc levels and some tissue samples removed at different intervals of time from their prostate, testis, and seminal vesicle. The zinc contents of these samples have been determined using the isotopic dilution analysis technique applied with 65Zn. In this way, the zinc contents of tissues varying between 0.02-4.50 μg Zn/g wet tissue could be determined with good precision. As a result, the zinc content of the prostate was found to be considerably high with respect to the zinc contents of the testis and the seminal vesicle. It is also interesting to note that a high zinc level in foods results in the accumulation of zinc in the prostate rather than in the testis and the seminal vesicle. The zinc content of the seminal vesicle decreases with a function of time passed from the beginning of the diet which contrasts with the case of prostate. It is also very remarkable that when the level of zinc in the body is low, the content of zinc in the testis is considerably higher. © 2009 Akadémiai Kiadó, Budapest, Hungary.
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    In vivo biodistribution of 131I labeled bleomycin (BLM) and isomers (A2 and B2) on experimental animal models
    (2010) AvcIbaşI U.; Demiroǧlu H.; Ünak P.; Müftüler F.Z.B.; Içhedef Ç.A.; Gümüşer F.G.
    Bleomycins (BLMs; BLM, A2, and B2) were labeled with 131I and radiopharmaceutical potentials were investigated using animal models in this study. Quality control procedures were carried out using thin layer radiochromatography (TLRC), high performance liquid chromatography (HPLC), and liquid chromatography (LC/MS/MS). Labeling yields of radiolabeled BLMs were found to be 90, 68, and 71%, respectively. HPLC chromatograms were taken for BLM and cold iodinated BLM (127I-BLM). Five peaks were detected for BLM and three peaks for 127I-BLM in the HPLC studies. Two peaks belong to isomers of BLM. The isomers of BLM were purified with using HPLC. Biological activity of BLM was determined on male Albino Wistar rats by biodistribution and scintigraphic studies were performed for BLMs by using New Zelland rabbits. The biodistribution results of 131I-BLM showed high uptake in the stomach, the bladder, the prostate, the testicle, and the spinal cord in rats. Scintigraphic results on rabbits agrees with that of biodistributional studies on rats. The scintigraphy of radiolabeled isomers (131I-A2 and 131I-B2) are similiarly found with that of 131I-BLM. © 2010 Akadémiai Kiadó, Budapest, Hungary.