Browsing by Author "Avcibasi U."

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    Comparison of the radiopharmaceutical potentials of dithizone radiolabeled with 131I and with 99mTc
    (2007) Ünak T.; Yildirim Y.; Avcibasi U.; Biber Z.; Duman Y.; Ünak P.
    In this study, dithizone (diphenylthiocarbazone) has been separately radiolabeled with 131I and with 99mTc for preliminarily testing their radiopharmaceutical potentials on male albino rabbits. 131I-dithizone and 99mTc-dithizone were intravenously injected to rabbits via their ear veins after anesthetizing with a mixture of Alfazyne and Alfamine (Serva) to determine their dynamic and static statuses in the metabolism. Also, 99mTc as pertechnetate and 131I as iodate were administered to rabbits as controls. Dynamic and static scintigrams were obtained using a gamma camera (Diacan Instruments). Dynamic scintigrams were obtained over the first half hour with frames of 1 minute following the administrations of the labeled compounds. Static images were obtained from posterior projection at different time intervals up to about 3 hours following the administration of the radiolabeled compounds. 99mTc-dithizone was significantly uptaken by the pancreas in contrast to free 99mTc. In the case of 131I-dithizone, the distribution of 131I activity in the metabolism was clearly different than the case of free 131I and the uptake of 131I-dithizone at the pancreas zone was also significant. These preliminary tests have clearly indicated that especially 99mTc-dithizone has a significant potential to be used as a pancreatic radiopharmaceutical. © 2007 Springer Science+Business Media, Inc.
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    Iodine content in breast milk samples in the Aegean region in Turkey
    (Chemical Publishing Co., 2008) Avcibasi U.; Altunata T.
    In this study, the iodine content in breast milk was measured by Sandell-Kolthoff s method. The milk was obtained from 40 healthy mothers, aged 20 to 37, from the pediatric division of Ege University hospital. All mothers were from Aegean region of Turkey. Comparisons were made with commercial milk samples. Destruction of organic matter by using alkaline ash method was carried out prior to determination by Sandell-Kolthoff reaction based on iodine's catalytic effect on the Ce(IV)-As(III) system. The iodine concentration ranges were within 19.1 and 94.2 μg/kg in breast milk samples. Results obtained in this study clearly showed that breast milk samples did not have iodine deficiency which can lead to goiter and iodine concentrations of market milk were at sufficient level in the Aegean region of Turkey.
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    Novel 99mTc(III)-azide complexes [99mTc(N3)(CDO)(CDOH)2B-R] (CDOH2 = cyclohexanedione dioxime) as potential radiotracers for heart imaging
    (Elsevier Inc., 2016) Liu M.; Zheng Y.; Avcibasi U.; Liu S.
    Introduction In this study, novel 99mTc(III)-azide complexes [99mTc(N3)(CDO)(CDOH)2B-R] (99mTc-ISboroxime-N3: R = IS; 99mTc-MPboroxime-N3: R = MP; 99mTc-PAboroxime-N3: R = PA; 99mTc-PYboroxime-N3: R = PY; and 99mTc-Uboroxime-N3: R = 5U) were evaluated as heart imaging agents. Methods Complexes [99mTc(N3)(CDO)(CDOH)2B-R] (R = IS, MP, PA, PY and 5U) were prepared by ligand exchange between NaN3 and [99mTcCl(CDO)(CDOH)2B-R]. Biodistribution and imaging studies were carried out in Sprague–Dawley rats. Image quantification was performed to compare their initial heart uptake and myocardial retention. Results 99mTc-ISboroxime-N3, 99mTc-PYboroxime-N3 and 99mTc-Uboroxime-N3 were prepared with high RCP (93–98%) while the RCP of 99mTc-MPboroxime-N3 and 99mTc-PAboroxime-N3 was 80–85%. The myocardial retention curves of 99mTc-ISboroxime-N3, 99mTc-PYboroxime-N3 and 99mTc-Uboroxime-N3 were best fitted to the bi-exponential decay function. The half-time of the fast component was 1.6 ± 0.4 min for 99mTc-ISboroxime-N3, 0.7 ± 0.1 min for 99mTc-PYboroxime-N3 and 0.9 ± 0.4 min for 99mTc-Uboroxime-N3. The 2-min heart uptake from biodistribution studies followed the ranking order of 99mTc-ISboroxime-N3 (3.60 ± 0.68%ID/g) > 99mTc-PYboroxime-N3 (2.35 ± 0.37%ID/g) ≫ 99mTc-Uboroxime-N3 (1.29 ± 0.06%ID/g). 99mTc-ISboroxime-N3 had the highest 2-min heart uptake among 99mTc radiotracers revaluated in SD rats. High quality SPECT images were obtained with the right and left ventricular walls being clearly delineated. The best image acquisition window was 0–5 min for 99mTc-ISboroxime-N3. Conclusion Both azide coligand and boronate caps had significant impact on the heart uptake and myocardial retention of complexes [99mTc(N3)(CDO)(CDOH)2B-R]. Among the radiotracers evaluated in SD rats, 99mTc-ISboroxime-N3 has the highest initial heart uptake with the heart retention comparable to that of 99mTc-Teboroxime. 99mTc-ISboroxime-N3 is a promising alternative to 99mTc-Teboroxime for SPECT MPI. © 2016 Elsevier Inc.
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    Investigation of Bioactivity of Estragole Isolated from Basil Plant on Brain Cancer Cell Lines Using Nuclear Method
    (Bentham Science Publishers, 2023) Avcibasi U.; Dewa M.T.; Karatay K.B.; Kilcar A.Y.; Muftuler F.Z.B.
    Background: In recent years, there has been a significant increase in studies investigating the potential use of plant-origin products in the treatment and diagnosis of different types of cancer. Methods: In this study, Estragole (EST) was isolated from basil leaves via ethanolic extraction using an 80% ethanol concentration. The isolation process was performed using the High Performance Liquid Chromatography (HPLC) method. The EST isolated from the basil plant was radio-labeled with131I using the iodogen method. Quality control studies of the radiolabeled EST (131I-EST) were carried out by using Thin Layer Radio Chromatography (TLRC). Next, in vitro cell, culture studies were done to investigate the bio-affinity of plant-originated EST labeled with131I on human medulloblastoma (DAOY) and human glioblastoma-astrocytoma (U-87 MG) cell lines. Finally, the cytotoxicity of EST was determined, and cell uptake of131I-EST was investigated on cancer cell lines by incorporation studies. Results: As a result of these studies, it has been shown that131I-EST has a significant uptake on the brain cells. Conclusion: This result is very satisfying, and it has encouraged us to do in vivo studies for the molecule in the future. © 2023 Bentham Science Publishers.