Browsing by Author "Aydin, ZU"

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    Comparative phytochemical studies on the roots of Polygala azizsancarii and P. peshmenii and neuroprotective activities of the two xanthones
    Çalis, I; Becer, E; Ünlü, A; Aydin, ZU; Hanoglu, A; Vatansever, HS; Dönmez, AA
    Six known sucrose mono-, di- and triesters and five xanthone derivatives were isolated from the roots of Polygala peshmenii Eren, Parolly, Raus & Kurschner which is a narrow species endemic to Turkiye. Among the xanthones, 1,7-dihydroxy-2,3-methylenedioxy-5,6-dimethoxy-xanthone is an undescribed compound isolated for the first time from a natural source. The studies on the roots of P. azizsancarii Do & BULL;nmez have resulted in the isolation of four known compounds including sucrose mono-, di- and triesters. The structures of the sucrose esters and xanthones isolated from P. azizsancarii and P. peshmenii were established by spectroscopic methods, including 1DNMR (1H NMR, 13C NMR, DEPT-135), 2D-NMR (COSY, NOESY, HSQC, HMBC). Neuroprotective activities of two xanthones, 1,3,6-trihydroxy-2,5,7-trimethoxyxanthone and 3-O-& beta;-D-glucopyranosyloxy-1,6-dihydroxy-2,5,7-trimethoxyxanthone isolated from the roots of P. azizsancarii were evaluated in vitro using in a cellular model of Alzheimer's disease. SKNAS human neuroblastoma cells were used in the study and treated with different consecrations of A & beta;25-35 oligomer for up to 48 h. Cell viability was evaluated using MTT assay. The distribution of & beta;-amyloid, & alpha;-synuclein, tau, JAK2, STAT3, caspase 3 and BMP-2 were investigated using indirect immunoperoxidase staining. Our results suggested that both xanthones control tau aggregation with no effect on & beta;-amyloid plaque formation. In addition, for neuronal pathophysiology in AD cell model, decreased distributions of JAK/ STAT3 and BMP2 signaling pathways were demonstrated, therefore they play a role in the protective effect on neurons in neurodegenerative disease. A significant decrease in caspase 3 immunoreactivity was detected after the administration of both compounds in AD cells. Therefore, both compounds control neuronal pathophysiology and rescue cell death in AD disease.
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    The cytotoxic and antiproliferative effect of Polygala saponin XLIV on the human colorectal carcinoma cell line
    Becer, E; Hanoglu, A; Ünlü, A; Aydin, ZU; Dönmez, AA; Jurt, S; Vatansever, SH; Çalis, I
    Objectives Saponins are secondary metabolites naturally found in plants with diverse pharmacological properties such as anticancer. This research aimed to explore the anti-cancer properties of Polygalasaponin XLIV (PS-XLIV) in a human colorectal carcinoma cell line derived from Polygala vulgaris roots.Methods HCT166 cells were treated with different PS-XLIV concentrations and incubated for 24 and 48 h. We used immunocytochemistry to investigate PS-XLIV's anti-cancer properties, employing antibodies targeting WNT3A, WNT11, STAT3, beta-catenin, and Ki-67. The IC50 value of PS-XLIV was 80 mu g/mL in HCT116 cells. WNT11, STAT3, beta-catenin, and Ki-67. Immunoreactivities significantly decreased in PS-XLIV-treated HCT116 cells than in control group cells.Results After PS-XLIV treatment, the epithelial morphology of cells was protected; however, the number of cells was less than that of the control group cells. While WNT3A immunoreactivity was similar in both groups, WNT11 and beta-catenin immunoreactivities were decreased after PS-XLIV application. In addition, the PS-XLIV treated group exhibited significantly weaker Ki-67 immunoreactivity, STAT3 immunoreactivty was moderated after PS-XLIV application.Conclusions For the first time, the anticancer effects of PS-XLIV isolated from P. vulgaris on HCT116 cells were shown. The anticancer effect may involve PS-XLIV reducing WNT11, beta-catenin, STAT3, and Ki-67 activation pathways in HCT116 cells.