Browsing by Author "Büyük, I"

Now showing 1 - 3 of 3
  • No Thumbnail Available
    Item
    MORPHOLOGICAL, PALYNOLOGICAL AND PHYLOGENETIC RELATIONSHIPS OF GLAUCIUM MILL. IN TURKEY
    Kiliç, FM; Yildiz, K; Batir, MB; Kiliç, M; Büyük, I
    Glaucium taxa were investigated in terms of their morphological, palynological and phylogenetical characteristic. The results of this study show differences between the taxa in some of these characteristics, especially in micromorphology and formation of clades in phylogenetic trees based on the matK and ITS3-6 DNA sequence data. Based on the findings of the molecular analyses supported by morphological data (stem's trichomes), the genus Glaucium of Turkey was divided into subsections Glabrousae and Pubescentae.
  • No Thumbnail Available
    Item
    Determination of the DNA changes in the artichoke seedlings (Cynara scolymus L.) subjected to lead and copper stresses
    Batir, MB; Candan, F; Büyük, I
    This study aims at determining the effects of lead (Pb) and copper (Cu) on the hyperaccumulator artichoke. The effect of Pb and Cu toxicity with different levels of concentrations (20, 40, 80, 160, 240, 320, 640 and 1280 ppm) caused a decrease in the root length and total soluble protein of the artichoke. As a result of treatment with the Pb and Cu solutions, the changes occurred in RAPD profiles of seedlings and revealed variations like increment and/or loss of bands compared to the control plants. These changes showed a decrease in genomic template stability (GTS, changes in RAPD profile) caused by genotoxicity. RAPD data and GTS values seemed consistent with the results of the root length measurements and total soluble protein analysis. In addition, it was seen that the genomic template stability was significantly affected by direct proportion of primary root length, root dry weight and root total soluble protein content in artichoke subjected to Pb and Cu stresses. As a result, it can be concluded that RAPD analysis based on the used primers in the current study can be applied in combination with physiological and biochemical parameters to measure genotoxic effects of lead and copper on artichoke plants.
  • No Thumbnail Available
    Item
    Association Between Idiopathic Generalized Epilepsy and EFHC1 Gene Mutations of 662 G>A and 685 T>C
    Büyük, I; Tugrul, B; Yilmaz, H; Onur, E; Vatandas, G; Dogan Bozyigit, F
    Objective: Idiopathic generalized epilepsy (IGE) is an epilepsy form without an underlying brain lesion or neurological indication or symptom. Recent investigations on the genetic origins of IGE and its subtypes report that certain mutations of various ion and non-ion channel genes in the central nervous system may be associated with IGE. Among these mutations, the ones related to the non-ionic channel gene EFHC1 are controversial (545G>A, 685T>C, 628G>A 757G>T, 229C>A, 662G>A, 520A>G, 776G>A, 829C>T). In this study we investigated the relationship between IGE and 662G>A (R221H) and 685T>C (F229L) mutations in EFHC1 gene in a Turkish population. Material and Methods: The study enrolled 96 healthy volunteers (47 male, 49 female) and 96 IGE patients (41 male, 55 female). IGE diagnosis was confirmed in the neurology department. After venous blood sampling, DNA extractions were performed. The presence of 662G>A (R221H) and 685T>C (F229L) mutations in the exon 4 of EFHC1 gene were analyzed using Real-Time polymerase chain reaction (PCR) (Cobas, Roche Diagnostics, Germany). The results of the control and patient groups were compared statistically. Results: In the patient group there was one heterozygous male with 685T>C mutation. In the control group, there were two subjects with 685T>C mutation; one heterozygous male and one heterozygous female. The control and the patient groups did not have the 662G>A mutation. The difference between the patient and the control groups were not significant (p value for 685 T>C mutation=0.56062; p value for 662G>A mutation=1.00). Conclusion: We found no evidence that EFHC1 is a major genetic factor for the development of IGE in Turkish patients. Our results indicated that 685T>C and 662G>A mutations might not be associated with IJE.