Browsing by Author "Baka, M"

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    Differentation of human spermatogenetic stem cells from azospermia patients to make sperm-like cells
    Gozuacik, D; Vatansever, HS; Kara, B; Calimlioglu, N; Yasar, P; Tavmergen, E; Goker, ET; Semerci, B; Baka, M; Ozbilgin, K
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    Neurotoxicity of chronic epidural somatostatin administration in rabbits
    Tekin, I; Mirzai, H; Yentur, EA; Ates, U; Baka, M; Yurtseven, M
    The central administration of somatostatin (SMS) in humans became a subject of controversy on the issue of potential neurotoxicity on the spinal cord. The study was aimed at the assessment of the neurodegenerative effects of chronic epidural SMS administration in rabbits. Rabbits were randomly assigned to two groups: the SMS and the control group. The SMS group received 100 mug SMS and the control group received isotonic saline by epidural catheter for 15 consecutive days. Then, laminectomy was performed and the spinal cord was removed. Light and electron microscopic examinations were performed. In the control group, a mild dural inflammatory response and in the SMS group, loss of Nissl bodies at the pericarion, chromatolysis and shrinking at nucleus membranes were observed in all animals at different degrees in light microscopy. In electron microscopy, mitochondrial swellings, irregularities in both nucleus and cell membrane, splitting at myelin lamellae, degeneration at myelin sheath and shrinking of axolemma were found in the SMS group. Our results showed the neurotoxic effects of chronic administration of SMS in rabbits both in light and electron microscopy even in a quite low doses. However, there were no significant clinical findings for the neurological effects during long term follow up.
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    Repeated epidural injections of ketamine with preservative benzethonium chloride produce evidence for neurotoxicity in rabbits
    Yentur, EA; Mirzai, IT; Mirzai, H; Ates, U; Baka, M; Yurtseven, M
    Background and objectives: In this study, we investigated whether repeated doses of 1% ketamine with preservative benzethonium chloride, administered into the epidural space of the rabbit, caused direct neurotoxicity. Methods: Twelve rabbits were randomly assigned to two groups (ketamine and control). After the animals were anesthetized, lumbar epidural catheters were placed for repeated epidural drug delivery. The ketamine group received 1% ketamine with preservative benzethonium chloride (0.5 ml) and the control group received isotonic saline (0.5 ml) once a day for 14 consecutive days. The day after the last injection, the animals were reanaesthetized, the left and right ventricles were cannulated and perfused with 2% glutaraldehyde, 1% formaldehyde mixture, in 0.1 mol/l phosphate buffer. Then, laminectomy was performed. A five centimetre segment of the spinal cord was removed and examined by light and electron microscopy to observe possible histological changes. Microscopic examinations were performed by coding each animal by a neuro-histologist who was blinded as to the source of each specimen. Results: Ketamine-treated rabbits showed significant histological changes at light and electron microscopy findings compared with the control group (p < 0.05). Conclusions: These changes suggested a neurotoxic effect of ketamine with preservative benzethonium chloride following chronic epidural administration.