Browsing by Author "Baklan B."

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    Identification of the variations in the CPT1B and CHKB genes along with the HLA-DQB1*06:02 allele in Turkish narcolepsy patients and healthy persons
    (Mary Ann Liebert Inc., 2014) Cingoz S.; Agilkaya S.; Oztura I.; Eroglu S.; Karadeniz D.; Evlice A.; Altungoz O.; Yilmaz H.; Baklan B.
    Background: The HLA-DQB1*06:02 allele across all ethnic groups and the rs5770917 variation between CPT1B and CHKB genes in Japanese and Koreans are common genetic susceptibility factors for narcolepsy. This comprehensive genetic study sought to assess variations in CHKB and CPT1B susceptibility genes and HLA-DQB1*06:02 allele status in Turkish patients with narcolepsy and healthy persons. Methods: CHKB/CPT1B genes were sequenced in patients with narcolepsy (n=37) and healthy persons (n=100) to detect variations. The HLA-DQB1*06:02 allele status was determined by sequence specific polymerase chain reaction. Results: The HLA-DQB1*06:02 allele was significantly more frequent in narcoleptic patients than in healthy persons (p=2×10-7) and in patients with narcolepsy and cataplexy than in those without (p=0.018). The mean of the multiple sleep latency test, sleep-onset rapid eye movement periods, and frequency of sleep paralysis significantly differed in the HLA-DQB1*06:02-positive patients. rs5770917, rs5770911, rs2269381, and rs2269382 were detected together as a haplotype in three patients and 11 healthy persons. In addition to this haplotype, the indel variation (rs144647670) was detected in the 5′ upstream region of the human CHKB gene in the patients and healthy persons carrying four variants together. Conclusion: This study identified a novel haplotype consisting of the indel variation, which had not been detected in previous studies in Japanese and Korean populations, and observed four single-nucleotide polymorphisms in CHKB/CPT1B. The study confirmed the association of the HLA-DQB1*06:02 allele with narcolepsy and cataplexy susceptibility. The findings suggest that the presence of HLA-DQB1*06:02 may be a predictor of cataplexy in narcoleptic patients and could therefore be used as an additional diagnostic marker alongside hypocretin. © 2014 Mary Ann Liebert, Inc.
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    Prevalence of sleep disorders in the Turkish adult population epidemiology of sleep study
    (Springer, 2015) Demir A.U.; Ardic S.; Firat H.; Karadeniz D.; Aksu M.; Ucar Z.Z.; Sevim S.; Ozgen F.; Yilmaz H.; Itil O.; Peker Y.; Aygul F.; Kiran S.; Gelbal S.; Cepni Z.; Akozer M.; Neyal A.; Cilli A.; Ozsancak A.; Kutlu A.; Salepci B.; Baklan B.; Oktay B.; Tuncel D.; Levent E.; Ekinci E.; Eyuboglu F.; Yildiz F.; Kirbas G.; Kaynak H.; Aydin H.; Boyaci H.; Bora I.; Oztura I.; Aslan K.; Gunhan K.; Habesoglu M.A.; Unlu M.; Demet M.; Dursunoglu N.; Tascilar N.; Yavuz N.; Erdinc O.; Araz O.; Dogan O.T.; Yetkin O.; Celik P.; Alp R.; Altin R.; Bilgin S.; Ismailogullari S.; Gazioglu S.; Ozkurt S.; Velioglu S.; Yetkin S.; Kuyucu T.; Atay T.; Uygunoglu U.; Tutar U.; Celik Y.; Bulbul Y.
    Sleep disorders constitute an important public health problem. Prevalence of sleep disorders in Turkish adult population was investigated in a nationwide representative sample of 5021 Turkish adults (2598 women and 2423 men, response rate: 91%) by an interviewer-administered questionnaire. Insomnia was defined by the DSM-IV criteria, habitual snoring and risk for sleep-related breathing disorders (SDB) by the Berlin questionnaire, excessive daytime sleepiness (EDS) by the Epworth sleepiness scale score, and restless legs syndrome (RLS) by the complaints according to the International Restless Legs Syndrome Study Group criteria. Mean age of the participants was 40.7 ± 15.1 (range 18 to 90) years. Prevalence rates (men/women) were insomnia 15.3% (10.5%/20.2%; P < 0.001), high probability of SDB 13.7% (11.1%/20.2%; P < 0.001), EDS 5.4% (5.0%/5.7%; P: 0.09), RLS 5.2% (3.0%/7.3%; P < 0.001). Aging and female gender were associated with higher prevalence of sleep disorders except for habitual snoring. Prevalence rates of the sleep disorders among Turkish adults based on the widely used questionnaires were close to the lower end of the previous estimates reported from different parts of the world. These findings would help for the assessment of the health burden of sleep disorders and addressing the risk groups for planning and implementation of health care. Sleep and Biological Rhythms © 2015 Japanese Society of Sleep Research.