Browsing by Author "Balcioğlu İ.C."

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    The in vitro effects of azithromycin and Clarithromycin on promastigotes and amastigotes of Leishmania tropica; [Leishmania tropica promastigotları ve amastigotları üzerine azitromisin ve klaritromisinin in vitro etkisi]
    (Veteriner Fakultesi Dergisi, 2012) Balcioğlu İ.C.; Ok Ü.Z.; Özbel Y.; Girginkardeşler N.; Özbilgin A.
    Leishmania (L.) tropica is one of the most common species responsible for cutaneous leishmaniasis (CL) in the Old World including Turkey. The pentavalent antimonials are widely used as intralesional and/or intramuscular in the treatment of CL, but increase in resistance to these agents led to investigations on alternative drugs. In vitro antileishmanial activities of two macrolides, azithromycin and clarithromycin were evaluated on promastigotes in RPMI 1640 medium and amastigotes in macrophage series of L. tropica. ED50 values of azithromycin and clarithromycin were found to be 5 µg/ml and <5 µg/ml on promastigotes, and 50-75 µg/ml and <3 µg/ ml on amastigotes, respectively, while ED90 values of the same drugs were 75 µg/ml and 25 µg/ml on promastigotes and 100 µg/ml and 10 µg/ml on amastigotes, respectively. Our data suggested that clarithromycin and azithromycin were effective on both L. tropica promastigotes and amastigotes in vitro. Clarithromycin was found to be more effective than azithromycin at lower concentrations on promastigotes and amastigotes. In vivo studies should be planned to detect intracellular concentrations of these drugs for the effective route and dosage. © 2012, Veteriner Fakultesi Dergisi. All rights reserved.
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    Are regular controls conducted in schools adequate in lowering the incidence of head lice (Pediculus capitis) infestation?; [Okullarda düzenli aralıklarla gerçekleştirilen kontroller saç biti (Pediculus capitis) insidansını düşürmekte yeterli olabilir mi?]
    (Veteriner Fakultesi Dergisi, 2012) Balcioğlu İ.C.; Kurt Ö.; Limoncu M.E.; Ermiş V.Ö.; Tabak T.; Oyur T.; Muslu H.; Kavur H.; Görgün S.; Girginkardeşler N.; Yereli K.; Bilaç C.; Özbel Y.
    In the present study, school children of similar age-groups from two villages of Manisa province, namely Osmancalı and Maldan, were examined in regular time periods to monitorize the changes in the incidence of head lice (Pediculus capitis). A total of 2091 children (1565 in Osmancalı and 526 in Maldan) were combed with a newly-designed lice comb (PDC®, KSL Consulting, Helsinge, Denmark) four times in May and November in 2008 and 2009. The assessment of the results was conducted with SPSS® v.15 program using CHAID and logistic regression analysis. The results of the periodic examinations of the children showed that the infestation rate of girls were found to be higher significantly compared to boys (P<0.001), and the incidence of head lice infestation in boys from Osmancalı was found three times higher than the incidence in boys in Maldan (P=0.002). In addition, regular dry combing and treatment lowered the infestation rates among girls significantly (P=0.001). According to the results of the study, regular dry combing of school children with specially-designed lice combs may be essential for the control of this common infestation, especially in rural areas. © 2012, Veteriner Fakultesi Dergisi. All rights reserved.
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    Assessment of in-vitro antileishmanial activities of Cynara scolymus extracts against Leishmania tropica; [Leishmania tropica’ya karşı Cynara scolymus ekstrelerinin in-vitro antileishmanial etkinliğinin değerlendirilmesi]
    (Veteriner Fakultesi Dergisi, 2021) Yildirim A.; Aksoy T.; Ceylan Ş.S.; Kayalar H.; Tayfur E.; Balcioğlu İ.C.
    It was aimed to investigate in vitro antileishmanial activities of the receptacle, bractea, and stem leaves extracts of Cynara scolymus (artichoke) against Leishmania tropica. The Leishmania isolate, isolated from a cutaneous leishmaniasis patient from Manisa province, Turkey and stored in liquid nitrogen, was identified as L. tropica (MHOM/TR/2012/CBCL-LT) by genotyping. In vitro antileishmanial activities of C. scolymus plant extracts were examined by CelltTiter-glo and hemocytometry, and cytotoxic activities by MTT. IC50 values of receptacle water (WRC), aqueous ethanol (ARC) and ethanol (ERC), bractea leaf water (WBC), aqueous ethanol (ABC) and ethanol (EBC), and stem leaf water (WSC), aqueous ethanol (ASC) and ethanol (ESC) extracts were determined as 2.45 mg/mL, 1.52 mg/mL, 1.66 mg/mL, 3.45 mg/mL, 1.46 mg/mL and 0.58 mg/mL, 0.24 mg/mL, 0.21 mg/mL and 0.08 mg/mL, respectively. When these results are compared with the drug-free control group, it was determined that stem leaf aqueous ethanol (SI: 7.98), ethanol (SI: 4.96) and water (SI: 2.71) extracts with the highest selectivity index (SI) values showed antileishmanial activity (P<0.05). Extracts of C. scolymus did not show cytotoxic activity except for WBC, WRC and ARC. In conclusion, the data presented in the current study indicated that C. scolymus stem leaf extracts (ESC, ASC and WSC) present effective antileishmanial activity. Future studies could focus on the identification and purification of the antileishmanial compounds within these extracts for analysis of their in vivo antileishmanial activity. © 2021, Veteriner Fakultesi Dergisi. All rights reserved.