Browsing by Author "Baser, KHC"

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    Boswellia sacra essential oil manages colon cancer stem cells proliferation and apoptosis: a new perspective for cure
    Becer, E; Kabadayi, H; Baser, KHC; Vatansever, HS
    Boswellia sacra Flueck essential oil is obtained from frankincense obtained from Boswellia sacra trees. The aim of this study was to investigate the in vitro anti-cancer activity of Boswellia sacra (BS) essential oil in CD133+ and CD133- Colo-320 cells. Essential oil was analyzed by GC-MS. CD133+ cells, cancer stem cells, were obtained from the Colo-320 by the MiniMACS system. Cytotoxicity were evaluated with MTT assay. Anti-cancer activity of BS essential oil was investigated by immunocytochemistry. 41 compounds were identified in the essential oil. 1:2 dilution BS essential oil was found to be active against all the cells for 24 h incubation. beta-catenin immunoreactivity was significantly decreased in both BS essential oil-treated CD133+ and CD133- Colo-320 cells. Caspase-3 immunoreactivity significantly increased in BS essential oil-treated CD133- Colo-320 cells. We conclude that BS essential oil decreased beta-catenin signaling molecules which play a crucial role in cancer cell proliferation. BS essential oil promoted apoptosis in CD133- Colo-320 cells.
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    Antiproliferative, antioxidant and anti-inflammatory effects of Boswellia Sacra on human pancreatic cancer cells
    Becer, E; Altundag, EM; Özbilenler, C; Vatansever, HS; Baser, KHC
    Pancreatic cancer is a highly lethal and malignant tumor with high occurrence worldwide. Natural products such as essential oils with a lack of side effects may benefit pancreatic cancer supplementary therapy. Essential oil of BS was prepared by distillation of the gum resin and also has been shown to have anti-cancer and antioxidant activities. The present study aimed to determine the anti-cancer, antioxidant and anti-inflammatory activities in PANC-1 cells. Chemical Functional Moieties of Boswellia sacra essential oil were determined by Fourier transform infrared (FTIR) spectroscopy. The essential oil was simultaneously analyzed by GC/MS and GC-FID. Cell viability was determined by MTT assay. Anti-cancer activity of BS essential oil was investigated by immunocytochemistry using antibodies against STAT-3, IHH, beta-catenin, CD133, and Ki-67. The antioxidant activity of BS essential oil was tested by DPPH method. 41 compounds were identified in the essential oil. The IC50 value of BS essential oil was found as 130 mu g/mL in PANC-1 cells. Ki-67 immunoreactivity significantly decreased in BS essential oil-treated PANC-1 cells. STAT-3 and beta-catenin immunoreactivities significantly increased in BS essential oil-treated PANC-1 cells. Also, BS essential oil showed antioxidant and anti-inflammatory activities. We conclude that BS essential oil may suppress proliferation via the beta-catenin activation pathway in PANC-1 cells.
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    Composition and antibacterial, anti-inflammatory, antioxidant, and anticancer activities of Rosmarinus officinalis L. essential oil
    Becer, E; Altundag, EM; Güran, M; Vatansever, HS; Ustürk, S; Hanoglu, DY; Baser, KHC
    Rosemary is a culinary herb that is widely used in traditional medicine for its nutritional value and pharma-cological properties. The aim of this study was to investigate the composition, in vitro anticancer, antioxi-dant, and antibacterial activities of rosemary essential oil. Rosemary essential oil components were simultaneously analysed by GC/MS and GC/FID techniques. Different concentrations of rosemary essential oil were incubated for 24 and 48 h with HepG2 and EV304 cells. Cell viability was measured by MTT assay. Anti-cancer activities of rosemary essential oil were investigated by immunocytochemistry using antibodies directed against Ki-67, b-catenin, c-myc, Oct-3/4, and IL-8. Anti-inflammatory and antioxidant activity were evaluated with protein denaturation and DPPH assays, respectively. The anti-bacterial effect of rosemary essential oil was analysed by Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae with the broth microdilution and agar zone diffusion methods. Camphor (15.1%), verbenone (14.3%), a-pinene (13.6%), 1,8-cineole (11.8%), and borneol (7.9%) were determined as the major compounds of rosemary essential oil. The activities of the oil were found to be 508.7 mg/ml and 525.7 mg/ml against HepG2 and ECV304 cells, respec-tively. Ki-67, b-catenin, c-myc, Oct-3/4, and IL-8 immunoreactivities were significantly reduced in rosemary essential oil-treated HepG2 cells. Ki-67 and b-catenin immunoreactivities were significantly decreased only in rosemary essential oil-treated ECV304 cells. Also, the essential oil showed antioxidant and free radical scavenging activities. Rosemary essential oil showed effective antibacterial activity on E. coli and K. pneumo-niae. Thus, rosemary essential oil could be a potential candidate as a therapeutic agent in cancer treatment.& COPY; 2023 SAAB. Published by Elsevier B.V. All rights reserved.
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    Cytotoxic activity and antioxidant effects of Origanum onites essential oil and its two major contents, carvacrol and p-cymene on human colorectal (HCT116) and hepatocelluler carcinoma (HepG2) cell lines
    Becer, E; Altundag, EM; Baser, KHC; Vatansever, HS
    Origanum onites L. (O. onites) is one of the cultivated Origanum species in Turkey. The aim of this study was to determine the cytotoxicity and cellular antioxidant effects of O. onites essential oil, carvacrol and p-cymene on HCT116 and HepG2 cells. O. onites essential oil components were analyzed by GC/MS and GC/FID. Cytotoxicity was analyzed via the MTT assay in HCT116 and HepG2 cells. Cellular antioxidant activities of O. onites essential oil, carvacrol and p-cymene were determined with DCFH-DA method. GC/MS analysis identified carvacrol (%78.4) as the main constituent of O. onites essential oil. 400 mu g/ml concentration of O. onites essential oil, carvacrol and p-cymene were found to be active against HCT116 and HepG2 cells. Also, carvacrol showed higher cellular antioxidant activity than O. onites essential oil and p-cymene. We conclude that O. onites essential oil and carvacrol may be a candidate for pharmaceutical products in treatment of cancer.
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    Effect of dietary essential oil mixture on performance of laying hens in summer season
    Çabukt, M; Bozkurt, M; Alçiçek, A; Çatli, AU; Baser, KHC
    The experiment was conducted to evaluate the effects of dietary supplements of either an essential oil mixture (EOM) or a mannan oligosaccharide (MOS) as alternatives to an antibiotic feed additive (avilamycin) for layers kept under hot summer conditions. Four hundred and eighty 54-week old Nick-Brown hens were assigned to four dietary treatments. Each treatment consisted of four replications of 10 cages (three hens per cage). The treatment groups were: 1) Control: Basal diet without additive; 2) Basal diet plus antibiotic (10 mg avilamycin/kg feed); 3) Basal diet plus 1 g MOS/kg feed; 4) Basal diet plus 24 mg EOM/kg feed. Performance of laying hens was affected by dietary treatments. Dietary supplementation of EOM and MOS significantly increased egg production compared with control and antibiotic groups. There were no significant differences in feed consumption between treatments. The EOM significantly improved feed conversion ratio above that of the control group. Egg weights were significantly different between treatments. Laying hens consuming MOS produced significantly lower egg weights than the other groups, while egg weights in the EOM, antibiotic and control groups did not differ significantly. Cracked-broken egg rate was decreased by dietary addition of EOM, MOS and antibiotic compared with the control. Number of deaths among hens was significantly affected by dietary treatments with the number of deaths in the MOS treatment being significantly lower than in the other treatments. The performance of laying hens during the summer season could be maintained with inclusions of EOM and MOS in the diet.
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    The Effect of Cynara cornigera L. in HepG2 Hepatocellular Carcinoma Cells
    Sanlidag, E; Becer, E; Çalis, I; Baser, KHC; Hanoglu, A; Göger, F; Vatansever, HS
    Amongst all cancer types, liver cancer is the fourth leading cause of cancer mortality. It is frequently stated as hepatocellular carcinoma (HCC) and occurs in hepatocytes. Genetic alterations of hepatocytes such as Wnt/beta-catenin and JAK / STAT signaling pathways play a key role for the development of the HCC. Currently, there are a few available treatments for HCC; such treatments include transplantation, surgical resections and anticancer drugs. Most of the anti-cancer drugs target the signaling pathways for achieving an effective treatment. However, these treatments have some undesirable side effects. Thus, there is a need for discovering alternative anti-cancer agents with no or lesser side effects. Plant constituents are promising anti-cancer agents. Cynara cornigera L. contains plenty of phenolic compounds including quercetin, apigenin, etc. This study aimed to analyze the anti- cancer property of the fractionated methanol extract of the flowers of C. cornigera. All the fractions obtained were analyzed to determine the cytotoxic activity on HepG2 cells. Two of the fractions containing polyphenolic compounds had a significant cytotoxic activity related to non-canonical Wnt11 signaling pathways on HepG2 cells.
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    Composition of the Essential oil of Endemic Haplophyllum megalanthum Bornm. from Turkey
    Ünver-Somer, N; Kaya, GI; Sarikaya, B; Önür, MA; Özdemir, C; Demirci, B; Baser, KHC
    The composition of the essential oil produced from the flowering aerial parts of Haplophyllum megalanthum Bornm. (Rutaceae), endemic to Turkey, was analyzed by GC and GC-MS. Among the fifty-eight compounds constituting about 91.7 % of the essential oil, the main components were characterized as palmito-gamma-lactone (45.8 %), octadecatrienoic acid (10.7 %), linoleic acid (6.5 %), octadecatetraenoic acid (6.3 %) and nonacosane (4.8 %).
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    Traditional phytotherapy and trans-cultural pharmacy among Turkish migrants living in Cologne, Germany
    Pieroni, A; Muenz, H; Akbulut, M; Baser, KHC; Durmuskahya, C
    This article reports on an ethnopharmaceutical field study carried out among Turkish migrants in Cologne, western Germany, which recorded 79 botanical taxa and 115 plant-based preparations, encompassing 167 folk phytotherapeutical uses, as well as a few other biological (animal and mineral derived) remedies. One-fourth of the recorded remedies were represented by food-medicines. Half of the ingredients quoted came originally from Turkey; only a few plants were gathered from the wild or cultivated in the Cologne area. This article discusses the Turkish migrants' frequent use of aerial parts of Sideritis species, the fruits of Pistacia terebinthus and the seeds of Peganum harmala from the perspective of modern phytopharmacology, phytotherapy and toxicology. It considers cultural adaptation related to the use of folk pharmaceuticals by analysing the ingredients of the Turkish folk pharmacopoeia and comparing them with those of the Turkish medical ethnobotany and of the phytotherapy of the host country (Germany). Only one third of the remedies mentioned by Turkish migrants living in Cologne are also known in German evidence-based phytotherapy, and less than 10% of the recorded phytotherapeutic uses are common to both pharmacopoeias. This article concludes by suggesting hypotheses on future changes affecting the knowledge and use of medicinal plants within the Turkish community of Cologne. (C) 2005 Elsevier Ireland Ltd. All rights reserved.