Browsing by Author "Baykan, AR"

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    Assessment of inflammatory markers in ulcerative colitis and association with the disease
    (BAYRAKOL MEDICAL PUBLISHER) Baykan, AR; Cerrah, S; Karahan, B; Çiftel, S; Temiz, A; Kasap, E
    Aim: In daily routine practice, non-invasive tests are needed in addition to symptoms to determine activation in patients with ulcerative colitis (UC). In this study, it was aimed to compare the results of non-invasive tests used frequently at the time of diagnosis of patients diagnosed with UC with endoscopic severity. Material and Methods: This retrospective cohort study was carried out on 202 patients between 2018 and 2022. The white blood count (WBC), mean platelet volume (MPV), hemoglobin (HB), hematocrit (Hct), C-reactive protein (CRP), albumin, neutrophil-lymphocyte ratio (NLR), CRP-albumin ratio (CAR), platelet-lymphocyte ratio (PLR). and lymphocyte-monocyte ratio (LMR) of the patients were calculated at the time of diagnosis. The severity of UC was assessed via colonoscopy using the Mayo Endoscopic Subscore (Mayo Score). In addition, patients with active UC were re-evaluated during remission to calculate the predictive values of tests in UC activation and severity. Results: There was a significant correlation between the Mayo Subscore and CRP (r: 0.34, P < 0.01), WBC (r: 0.23, P = 0.01). HB (r: -023, P = 0.01), NLR (r: 0.49, P < 0.01), CAR (r: 0.51, P < 0.01), PLR (r 0.32, P < 0.01), and LMR (r: -0.34, P < 0.01). The sub-assessment, taking colon involvement into consideration, showed a correlation between the Mayo Subscore and NLR and CAR with pancolitis, left colon involvement, and distal colitis. The highest area under the curve (AUC) value, found in the tests, was with the CAR (0.919). When the CAR cut-off value was taken as 0.11, the sensitivity was 802%, and the specificity was 94.4%, indicating UC activation. Discussion: Inflammatory markers in UC have adequate sensitivity in indicating activation, and they also aid in the identification of severity of involvement in patients.
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    Mean platelet volume can indicate dietary adherence and disease severity of celiac disease
    (KARE PUBL) Gerceker, E; Baykan, AR; Cerrah, S; Yuceyar, H
    OBJECTIVE: At present, there is no reliable indicator for dietary compliance and disease severity in patients with celiac disease (CD). The aim of this study is to evaluate mean platelet volume (MPV) level as a biomarker for detection of disease activation, dietary adherence, and assessment of disease severity. METHODS: Eighty-one patients with CD and 50 healthy subjects were enrolled in this study. The diagnosis of CD was estab-lished by both positive antibodies against endomysium or gliadin and histopathological criteria (lymphocytic infiltration and total villous atrophy in duodenal biopsies). RESULTS: MPV was observed to be significantly higher among CD patients when compared to healthy controls (8.14 +/- 0.26 fL vs. 7.82 +/- 0.29 fL and p=0.001). Overall dietary adherence rate was 72.8% (58/81 CD patients). After induction of a gluten-free diet, the MPV was significantly lower in the dietary adherent group than non-adherent patients (7.86 +/- 0.17 fL vs. 8.07 +/- 0.30 fL and p=0.001). The increase of MPV was correlated with Marsh classification (Marsh 3 active CD vs. Marsh 2 active CD vs. Marsh 1 active CD; 8.32 +/- 0.27 fL vs. 8.12 +/- 0.19 fL vs. 7.98 +/- 0.19 fL; p=0.004 and p=0.009). CONCLUSION: Based on these data, we believe that increased MPV can provide additional benefit to screening in patients with CD. It can indicate the activation of the disease and adherence to the diet.
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    Potential role of chromatin remodeling factor genes in atrophic gastritis/gastric cancer risk
    (AVES) Bilgiç, F; Gerçeker, E; Boyacioglu, SÖ; Kasap, E; Demirci, U; Yildinm, H; Baykan, AR; Yüceyar, H
    Background/Aims: Atrophic gastritis (AG), intestinal metaplasia (IM), and Helicobacter pylori (HP) are the risk factors for the development of gastric cancer (GC). Chromatin remodeling is one of the epigenetic mechanisms involved in the carcinogenesis of GC. The purpose of this study was to investigate the expression profiles of defined chromatin remodeling genes in gastric mucosal samples and their values as gastric carcinogenesis biomarkers. Materials and Methods: In total, 95 patients were included in the study. Patients were divided into 3 groups as: GC group (n=34), AG group (n=36), and control group (n=25). AG group was further divided into subgroups based on the presence of HP and IM in gastric mucosa. Chromatin remodeling gene expressions were analyzed using real-time PCR (RT-PCR) array in all groups. Data were evaluated using the RT-gPCR primer assay data analysis software. Results: EED, CBX3, and MTA1 were more overexpressed, whereas ARID1A ING5, and CBX7 were more underexpressed in the AG and GC groups compared with the controls. No significant differences were observed between the AG and GC groups concerning the expression of these 6 genes, although the fold change levels of these genes in the GC group were well above than in the AG group. EEO, CBX3, and MTA1 were significantly more overexpressed in HP- and IM-positive AG subgroup compared with the HP- or IM-negative AG subgroup. Conclusion: In conclusion, our results provide an evidence of epigenetic alterations in AG. Expressions of EED, CBX3, MTA1, ARID1A, ING5, and CBX7 may be considered as promising markers to be used in GC screening for patients with AG.
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    Overexpressions of RHOA, CSNK1A1, DVL2, FZD8, and LRP5 genes enhance gastric cancer development in the presence of Helicobacter pylori
    (ELSEVIER) Demirci, U; Orenay-Boyacioglu, S; Kasap, E; Gerçeker, E; Bilgiç, F; Yüceyar, H; Yildirim, H; Baykan, AR; Ellidokuz, EB; Korkmaz, M
    Background and study aims: Intestinal metaplasia (IM), and Helicobacter pylori (HP) infection can be shown as risk factors in the development of gastric cancer (GC). WNT signaling pathway plays a critical role in carcinogenesis. However, the literature studies are limited on the significance of this pathway for the transition from IM to GC. Patients and methods: We aimed to investigate the importance of the genes of WNT signaling pathways diagnostic and prognostic markers in the presence and absence of HP in conversion from IM to GC. 104 patients, (GC group n = 35, IM group n = 45, control group n = 25) were included in this case-control study. Expression of genes in WNT signalling were searched in study groups with qRT-PCR array and qRT-PCR method. Data were analysed using PCR array data analysis software. Results: Statistically significant overexpression of RHOA, CSNK1A1, DVL2, FZD8 and LRP5 genes was detected in the GC and IM groups compared to the control group (p < 0.05). Statistically significant overexpression of RHOA, CSNK1A1, DVL2, FZD8 and LRP5 genes was observed in patients with metastatic GC compared to patients with GC without metastasis (p < 0.05). It was found that the RHOA, CSNK1A1, DVL2, FZD8 and LRP5 genes were statistically significantly over-expressed in diffuse GC patients compared to non-diffuse GC patients (p < 0.05). Statistically significant overexpression of RHOA, CSNK1A1, DVL2, FZD8 and LRP5 genes was detected in HP positive IM patients compared to HP negative IM patients (p < 0.05). Conclusion: Overexpression of RHOA, CSNK1A1, DVL2, FZD8 and LRP5 genes in IM may suggest that these genes are important markers in the development of IM and inflammation with HP. In addition, these genes are linked to tumor burden in the GC group. Consequently, we can conclude that these genes are poor prognosis biomarkers for GC and have the potential to be used as markers for future treatment monitoring.
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    A No-Biopsy Approach for the Diagnosis of Celiac Disease in Adults: Can It Be Real?
    (SPRINGERNATURE) Baykan, AR; Cerrah, S; Ciftel, S; Vural, MK; Kasap, E
    Background and objective Pediatric guidelines on the diagnosis of celiac disease (CD) have reported that the positivity of anti-endomysium antibodies in the presence of anti-transglutaminase antibodies (TGA) 10 times higher than normal is sufficient for the diagnosis. In this study, we aimed to evaluate whether this diagnostic process for children can also be applied to adult patients. Materials and methods We retrospectively examined patients aged >18 years who were diagnosed with CD. The results of serological tests and endoscopic biopsy were evaluated. Patients with more than one month of duration between celiac serology and endoscopy, those diagnosed with CD before admission, those on a gluten-free diet, and those with selective IgA deficiency were excluded from the study.Results A total of 269 patients were included in the study. TGA value was significantly higher in patients with villous atrophy (p<0.001) and positively correlated with mucosal damage (r=0.60, p<0.01). Considering the cut-off value of 100 U/mL (>10 ULN) for the TGA antibodies, in line with the criteria regulated by the European Society for Paediatric Gastroenterology, Hepatology, and Nutrition (ESPGHAN) for the diagnosis of CD, the sensitivity was 71.64%, the specificity was 100%, and the positive predictive value (PPV) was 100%. When the cut-off value was taken as 29.42 U/mL, the sensitivity was 100% and the specificity was 99.5%. For a TGA cut-off value of 52.7 U/mL (5.27 ULN), which determines the presence of partial or complete villous atrophy in the evaluation made considering mucosal damage, the sensitivity was 90%, the specificity was 100%, and the PPV was 100%.Conclusion Based on our findings, TGA titers were highly effective in demonstrating CD-related mucosal damage. This study endorses a biopsy-free strategy in adult patients in line with the ESPGHAN criteria. Local validation of test-specific thresholds will ensure that this approach has a significant impact on adult patients.