Browsing by Author "Bayraktar, O"

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    Wound Healing Effects of Various Fractions of Olive Leaf Extract (OLE) on Mouse Fibroblasts
    Erdogan, I; Bayraktar, O; Uslu, ME; Tüncel, Ö
    Olive (Olea europaea) leaf has been introduced as a potential therapeutic in wound healing owing to combined antioxidant and antimicrobial activity. Comparison of crude extract and its fractions in terms of antioxidant capacity, antimicrobial and cytotoxic activity to gain insight about cell migration rate under exogenous stress of H2O2, as a hallmark of wound healing constituted the objective of this study. Oleuropein-containing fraction exerted the highest cell migration rate among other fractions that contains hydroxytyrosol, verbascoside and luteolin, whilst treatment with high concentrations (50 mu g/ml) of this fraction simultaneously with H2O2 caused a dramatic decline in cell migration, resulting in the loss of cell adherence. Results overall indicated that active compounds caused an imbalance in redox signaling beyond a critical concentration. Comparison of fractions and crude extract also revealed that crude extract promoted cell migration by 20%, which may be attributed to synergistic effect of undefined phenolics.
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    Antioxidant, Antimicrobial, and Cytotoxic Activities of Condensate from Rf-Vacuum Timber Drying Process in the Forestry Industry
    Köse, MD; Uslu, ME; Bayraktar, O
    As the demand for dried timber has been increasing worldwide, drying of timbers is required to supply the demand in the manufacturing industries of furniture and other interior woodwork. During the drying process, some condensation will occur, resulting in liquids' emission, which requires disposal. In the liquid condensate, there are many substances at low concentrations that make their recovery uneconomic. After revealing the potential of the substances available in these condensates, biotechnological processes can be employed to produce high value-added products. This condensate contains tree sap, including proteins, enzymes, and many natural compounds such as terpenoids, alkaloids, phenolic compounds. Thus, it is considered that these natural compounds might be beneficial in pharmaceutical applications. Natural compounds such as phenolic compounds are bioactive substances that have antioxidant, antimicrobial, and cytotoxic activities. This study was aimed to characterize the condensate from the Rf-vacuum drying process of beech (Fagus sylvatica L.), walnut (Juglans regia L.), and restharrow (Onosis arvensis L.) in terms of antioxidant capacity, total phenol content, antimicrobial activity, and cytotoxicity. Obtained data revealed that condensates from the Rf-vacuum drying process inhibited the growth of both S. epidermidis and E. coli significantly.
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    Development and Characterization of Silk Fibroin-based Oral Films Containing Turmeric Extract as Dietary Supplement
    Uslu, ME; Bayraktar, O
    Dietary supplements have extreme importance. Due to the easily degradable nature of these supplements, the development and application of carrier systems in food technologies seem to be extremely important. Today, oral film technologies have gained importance due to their rapid and high absorption properties. This study chose silk fibroin (SF) as the main component due to its high biocompatibility. Turmeric extract has been added to the oral films as an active agent. The prepared films were analyzed with atomic force microscopy (AFM) and scanning electron microscopy (SEM) to reveal their morphological properties, and at the same time, the film thickness was measured. It has been found that the increase in extract amount is a factor that causes an increase in film roughness while causing a decrease in phase separation. It was observed that the film roughness increased twice with the addition of extract. The roughness of the films formed with 15% extract was measured as 28.5. The average roughness of the films formed without the use of extracts was observed as 15.7 mm. It was also observed that the Trolox equivalent antioxidant capacity (TEAC) was doubled for films containing 15% turmeric extract after one hour of release. The films exhibited different weight loss profiles after release as the amount of turmeric extract was changed. Disc diffusion experiments revealed that films containing turmeric extract exhibited antimicrobial effects.
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    Evaluation of the hemostatic activity of Equisetum arvense extract: the role of varying phenolic composition and antioxidant activity due to different extraction conditions
    Uslu, ME; Mele, A; Bayraktar, O
    Hemostasis is a procedure that causes bleeding to stop and is very important in injuries. Recent researches were focused on the discovery of plant hemostatic agents. Therefore, plant extracts were investigated as a major source as a hemostatic agent. In this research, Equisetum arvense extract was investigated as a candidate coagulatory agent. Effect of extraction parameters on coagulation was investigated. Although it has been stated in the literature that the extract of Equisetum arvense has anticoagulatory activity, it has been shown in this study that alteration of the extraction parameters changes the extract bioactivity from the anticoagulant to the coagulant.
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    Utilization of Eggshell Membrane and Olive Leaf Extract for the Preparation of Functional Materials
    Bayraktar, O; Galanakis, CM; Aldawoud, TMS; Ibrahim, SA; Köse, MD; Uslu, ME
    Eggshell membrane (ESM) is a natural proteinaceous by-product of the food industry, especially in the pasteurized egg industry, resulting in the availability of much discarded egg waste. In the literature, eggshell (ES) and ESM usage for their adsorbent properties to remove various organic and inorganic hazardous chemicals, especially from wastewater, has gained interest. In addition, agricultural (olive leaf) and food industry (eggshell and eggshell membrane) waste can together be valorized to produce value-added functional products. This study's objective was to evaluate the eggshell membrane's loading capacity for bioactive compounds obtained from olive leaf extract (OLE) in order to prepare functional biomaterial. In this study, waste eggshell membranes were used to adsorb the phenolic compounds from olive leaf extract to design functional biomaterials. Using the foam separation method, both separation of the eggshell membrane and adsorption of bioactive compounds to the eggshell membrane were achieved simultaneously. The characterization studies showed that OLE was successfully adsorbed to the eggshell membrane. Cytotoxicity and antimicrobial studies showed that prepared OLE-loaded membranes were functional materials with bioactive properties. In conclusion, ESM was determined as a promising protein in the production of functional antioxidative and antimicrobial food or dietary supplement after the adsorption of bioactive olive leaf polyphenols.