Browsing by Author "Becer E."
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Item Fatty acid composition and anticancer activity in colon carcinoma cell lines of Prunus dulcis seed oil(Taylor and Francis Ltd, 2017) Mericli F.; Becer E.; Kabadayi H.; Hanoglu A.; Hanoglu D.Y.; Yavuz D.O.; Ozek T.; Vatansever S.Context: Almond oil is used in traditional and complementary therapies for its numerous health benefits due to high unsaturated fatty acids content. Objectives: This study investigated the composition and in vitro anticancer activity of almond oil from Northern Cyprus and compared with almond oil from Turkey. Materials and methods: Almond oil from Northern Cyprus was obtained by supercritical CO2 extraction and analyzed by GC-MS. Almond oil of Turkey was provided from Turkish pharmacies. Different concentrations of almond oils were incubated for 24 and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by MTT assays. Anticancer and antiprolifetarive activities of almond oils were investigated by immunocytochemistry using antibodies directed against to BMP-2, β-catenin, Ki-67, LGR-5 and Jagged 1. Results: Oleic acid (77.8%; 75.3%), linoleic acid (13.5%; 15.8%), palmitic acid (7.4%; 6.3%), were determined as the major compounds of almond oil from Northern Cyprus and Turkey, respectively. In the MTT assay, both almond oils were found to be active against Colo-320 and Colo-741 cells with 1:1 dilution for both 24 h and 48 h. As a result of immunohistochemical staining, while both almond oils exhibited significant antiproliferative and anticancer activity, these activities were more similar in Colo-320 cells which were treated with Northern Cyprus almond oil. Discussion and conclusion: Almond oil from Northern Cyprus and Turkey may have anticancer and antiproliferative effects on colon cancer cells through molecular signalling pathways and, thus, they could be potential novel therapeutic agents. © 2017 The Author(s).Item Apoptotic effects of Corchorus olitorius L. Leaf extracts in colon adenocarcinoma cell lines(Mattioli 1885, 2018) Soykut G.; Becer E.; Calis I.; Yucecan S.; Vatansever S.Corchorus olitorius L. is a highly consumed plant in Cyprus and other Eastern Mediterranean countries and known as a medicinal food in many cultures. The aim of the study is to determine in vitro anticancer and apoptotic induction effects of dichloromethane (DCM) and aqueous Corchorus olitorius L. leaf extracts in primary (Colo-320) and metastatic (Colo-741) colon adenocarcinoma cell lines.. Cell growth and cytotoxicity were measured with MTT assay with five different concentrations of extracts in Colo-320 and Colo-741 cell lines. Apoptotic activities of Corchorus olitorius L. were investigated by immunocytochemistry using antibodies directed against to caspase-3, cytochrome-c and FasLigand (FasL). TUNEL assay was used to detect DNA fragmentation in both cell lines. Both DCM and aqueous phase of extracts at 50 µg/ml concentration were more effective at inhibiting Colo-320 and Colo-741 cell growth when compared with other dilutions. The number of TUNEL positive cells was significantly higher in Colo-741 cells when compared with both control group and Colo-320 cell line. DCM phase extract significantly increased caspase-3 immunoreactivity while aqueous phase extract significantly increased cytochrome-c and FasLigand (FasL) immunoreactivities in Colo-320 cell lines. Both extracts were able to significantly increase caspase-3, cytochrome-c and FasL immunoreactivity in Colo-741 cells showing that both apoptotic pathways were triggered. Overall, Corchorus olitorius L. extracts induced apoptosis in both cancer cell lines while being more effective in metastatic colon adenocarcinoma cell lines suggesting that the extracts might have potential anticancer effects and possibility to be used as precursor to phytomedicinal colon cancer treatment as oppose to chemotherapy. © Mattioli 1885.Item The effect of Colchicum pusillum in human colon cancer cells via Wnt/β-catenin pathway(Elsevier B.V., 2019) Becer E.; Hanoğlu D.Y.; Kabadayı H.; Hanoğlu A.; Vatansever S.; Yavuz D.; Meriçli F.; Meriçli A.H.Objective: Colchicum pusillum belongs to the family Colchicaceae that particularly rich in tropolonic alkaloids. The aim of this study was to investigate the cytotoxicity and in vitro anticancer activity of Colchicum pusillum ethanolic extract on Colo-320 primer and Colo-741 metastatic colon adenocarcinoma cell lines. Materials and methods: Colchicum pusillum was collected and extracted with ethanol. Different concentrations of Colchicum pusillum extract were incubated for 24 h and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assays. Anticancer and antiproliferative activities of Colchicum pusillum were investigated by immunocytochemistry using antibodies directed against to β-catenin, Ki-67, LGR-5 Ki-67, DKK1, Frizzled-4, Wnt4, Wnt7a and caspase3 in Colo-741 cells. Results: All concentrations of Colchicum pusillum extract had toxic effect in Colo-320 cells. Because of this, we used Colchicum pusillum extract at 20 μg/ml for evaluate anticancer activities only in Colo-741 cells. As a result of immunohistochemical staining, β-catenin, LGR-5 and caspase-3 immunoreactivities were significantly increased while Wnt7a immunostaining intensity was decreased in Colo-741 cells. Conclusion We conclude that Colchicum pusillum extract increased β-catenin and LGR-5 via Wnt/β-catenin pathway in colon cancer cells. Interestingly, it decreased other signaling molecule, Wnt7a which is assumed to play protective role during carcinogenesis. Also, it increased significantly caspase-3 immunoreactivity showing that apoptotic pathways were triggered. © 2018Item Obtaining stem cell spheroids from foreskin tissue and the effect of corchorus olitorius l. On spheroid proliferation; [Sünnet derisinden kök hücre sferoidlerinin elde edilmesi ve corchorus olitorius l.’nin sferoid proliferasyonuna etkisi](Turkish Pharmacists Association, 2020) Becer E.; Soykut G.; Kabadayi H.; Mammadov E.; Çaliş İ.; Vatansever S.Objectives: Mesenchymal stem cells are self-renewing stem cells. The human foreskin has potential to be used as a source of stem cells. The aim of the study was to obtain spheroid formation of human foreskin cells (hnFSSCs) isolated from newborn human foreskin tissue. In addition, the apoptotic and proliferative effects of a traditional plant, Corchorus olitorius L. (C. olitorius), on hnFSSC spheroids were investigated. Materials and Methods: After a routine circumcision procedure the cells were isolated and cultured in suitable medium. The plant leaves was extracted with ethanol and their composition was analyzed by liquid chromatography coupled with mass spectrometry (LC-MS/MS). The foreskin stem cells were characterized immunocytochemically by CD45, CD34, and CD90 antibodies. hnFSSC spheroids were formed using the hanging drop technique. Immunofluorescence staining was used on the obtained spheroids to determine the distribution of caspase-3 and Ki-67 after being treated with C. olitorius extract for 48 h. Results: Immunostaining analysis showed that hnFSSCs were positive for CD45 and CD34 and negative for CD90. According to LC-MS/MS C. olitorius was rich in flavanols and hydrocinnamic acid derivatives. Although the spheroids obtained were loose and floating, the cells interacted with each other. Caspase-3 activity was higher in the control group than in the extract-treated group and Ki-67 was higher in the extract-treated group than in the control group, suggesting that the plant might have the capacity to increase stem cell proliferation due to its rich polyphenolic content. Conclusion: The results suggest that hnFSSCs and spheroids might be used in stem cell generation, tissue repair and renewal as human foreskin tissue has potential to be used as a stem cell source. C. olitorius also increased proliferation of hnFSSCs, showing that polyphenols might increase proliferation of stem cells. © Turk J Pharm Sci, Published by Galenos Publishing House.Item Quercetin-mediated apoptosis and cellular senescence in human colon cancer(Bentham Science Publishers, 2020) Özsoy S.; Becer E.; Kabadayı H.; Vatansever H.S.; Yücecan S.Background: Quercetin is a flavonol from the flavonoid group of polyphenols, which positively affects human health due to its anti-cancer, anti-inflammatory, anti-microbial and cardioprotective effects. The effects of phenolic compounds, including quercetin, on programmed cell death and cellular senescence, have been the subject of research in recent years. Objective: In this study, we aimed to investigate the effects of quercetin on cell viability, apoptosis and cellular senescence in primary (Colo-320) and metastatic (Colo-741) colon adenocarcinoma cell lines. Methods: Cytotoxicity was analyzed via MTT assay in Colo-320 and Colo-741 cell lines. After quercetin treatment, cell ularsenescence and apoptosis were evaluated by TUNEL staining, X-Gal staining and indirect peroxidase tech-nique for immunocytochemical analysis of related proteins such as Bax, Bcl-2, caspase-3, Hsp27, Lamin B1, p16, cyclin B1. Results: The effective dose for inhibition of cell growth in both cell lines was determined to be 25µg/ml quercetin for 48 hours. Increased Baximmunoreactivityfollowingquercetin treatment was significant in both Colo-320 and Colo-741 cell lines, but decreased Bcl-2 immunoreactivitywas significant only in theColo-320 primary cell line. In addition, after quercetin administration, the number of TUNEL positive cells and, immunoreactivities for p16, Lamin B1 and cyclin B1 in both Colo-320 and Colo-741 cells increased. Conclusion: Our results suggest that quercetin may only induce apoptosis in primary colon cancer cells. Further-more, quercetin also triggered senescence in colon cancer cells, but some cells remained alive, suggesting that colon cancer cells might have escaped from senescence. © 2020 Bentham Science Publishers.Item An endemic plant of Cyprus, origanum majorana: Is it A new alternative natural product for malaria treatment?; [Kıbrıs endemik bitkisi origanum majorana: Sıtma tedavisinde yeni bir alternatif doğal Ürün olabilir mi?](Ankara Microbiology Society, 2020) Güler E.; Özbilgin A.; Becer E.; Hanoğlu A.; Şanlidağ T.Malaria still remains to be a public health threat and one of the most important infectious diseases to get attention from World Health Organization. No domestic malaria cases have been reported on the island of Cyprus since 1948, as a result of successful elimination process. All of the malaria cases detected in recent years are imported cases. As known, hundreds of medicines are obtained from plants and traditional medicine are used in endemic places of malaria. The cause of malaria - Plasmodium parasites, are developing resistance to antimalarial drugs. Hence, research on plant extracts and essential oils have gained great interest in recent years to obtain new and safe agents/substances. In our study, it was aimed to investigate the in vivo antimalarial activities of essential oils obtained from Origanum dubium, Origanum majorana, Salvia fruticosa and Laurus nobilis plants which grows in Northern Cyprus against Plasmodium berghei - the rodent malaria agent. Plants were collected in appropriate seasons and were dried to obtain and analyze essential oils via Clevenger Apparatus system. L929 mouse fibroblast cell line and MTT [3-(4.5-dimethylthiazole-2-yl) -2.5-diphenyltetrazolium bromide] kit were used to determine the cytotoxic activities of the essential oils obtained. In our study, total of 36 mice (Balb/c) of 6 groups (6 mice in each group) were formed: chloroquine group (CG) (50 mg/kg) as malaria reference group, untreated control group (UTCG), O.dubium (OD) (20 mg/kg), O.majorana (OM) (20 mg/kg), S.fruticosa (SF) (20 mg/kg) and L.nobilis (LN) (20 mg/kg). The essential oils were given to mice infected with P.berghei strain orally on 0, 1, 2 and 3rd days (4 times in total). Blood was taken from the tail end of each mouse 24 hours after the last treatment and blood collection was continued every two days until the mice died. Withdrawn blood taken from the mice were prepared as a thin smear and stained with Giemsa. Then, parasitemia percentages in each smear were calculated. As a result of the cytotoxicity tests, cytotoxic activity was not found at 100 µg/ml (20 mg/kg) in all oils except OD essential oil. While the mice receiving chloroquine continued their lives with the disappearance of the parasite on the 6thday, the mice in the UTCG died on the 9th day. The parasitemia rate reached 35% in the OM group on the 23rd day, in the OD group on the 21st day and in the other groups (SF and LN) on the 14th day and the mice have died. In our study, the difference between the life span in all groups was found statistically significant (p≤ 0.001). As a result, the essential oils O.majorana (14 days increase according to UTCG) an endemic plant of Cyprus and O.dubium (12 days increase according to UTCG) which had an antimalarial effect, decreased parasitemia and increased the life span of mice more than two times, indicated that they could be a source for the acquisition of new antimalarial molecules. © 2020 Ankara Microbiology Society. All rights reserved.Item The investigation of antiproliferative effects of quercetin, curcumin and their combinations on breast cancer cell lines; [Kuersetin, kurkumin ve kombinasyonlarinin meme kanseri hücre hatlari üzerindeki antiproliferatif etkilerinin arastirilmasi](University of Ankara, 2020) Altundag E.M.; Becer E.; Gencąlp D.; Vatansever H.S.Objective: Breast cancer is a type of cancer that has high malignancy and mortality rates in women and is frequently seen in the Eastern Mediterranean region. Flavonoids such as quercetin and curcumin are known to have an ability to inhibit the growth of cancer cells. The aim of this study was to determine the antiproliferative effect of quercetin and curcumin which are flavonoids known to have anticancer properties on human metastatic and non metastatic breast cancer cell lines of alone and in combination usage. Material and Method: Cell growth and cytotoxicity of quercetin, curcumin and quercetin:curcumin combinations were measured with MTT assay both on M4A4 and MCF-7 breast cancer cell lines. Combosyn program was used to determine IC50 and combination index (CI) values. Result and Discussion: Half-maximal inhibitory concentrations (IC50) of quercetin for 72 hours were 49 μM and 120 μM on M4A4 and MCF-7 cells, respectively. IC50 doses for curcumin on M4A4 and MCF-7 cells were determined as 27 μM and 54 μM, respectively. It was determined that quercetin:curcumin combinations applied were shown a synergistic and antagonistic effect on MCF-7 and M4A4 cells. It was found that both quercetin and curcumin treatment inhibit cell viability in a dose and time-dependent manner. Findings show that combination with quercetin and curcumin polyphenol can be used in non-metastatic breast cancer, while different combination studies are needed for metastatic breast cancer. © 2020 University of Ankara. All rights reserved.Item Evaluation of cytotoxic effects of berberine-chloride in breast cancer cells and antimicrobial activity analysis; [Berberin-klorürün meme kanseri hücreleri üzerindeki sitotoksik etkilerinin değerlendirilmesi ve antimikrobiyal aktivite analizi](Turkiye Klinikleri, 2020) Altundağ E.M.; Becer E.; Şanlitürk G.; Güran M.; Vatansever H.S.Objective: In this study, it was aimed to analyse the anti-cancer activity of berberine-chloride in two different cancer cell lines (MCF-7 breast cancer cells and M4A4 metastatic breast cancer cells) and to investigate the antibacterial activity in five different standard bacterial strains. Material and Methods: In the study, the time-dependent inhibition by berberine chloride was investigated by applying MTT assay against two cell lines, which are MCF-7 breast cancer cells and M4A4 metastatic breast cancer cells. In addition, the anti-bacterial effect of berberine chloride was analysed by using six standard strains [Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, methicillin resistant S. aureus (MRSA), Pseudomonas aeruginosa and Enterococcus faecalis] with the broth microdilution and agar zone diffusion methods. Results: The concentration at which cell viability was 50% inhibited for MCF-7 cells after 72 hours of berberine chloride treatment was found to be 19.57 µM and 77.7 µM for M4A4 cells. Minimum inhibitory concentration values in broth microdilution test for S. aureus, MRSA, E. coli, K. pneumoniae, P. aeruginosa and E. faecalis, was found to be 0.49 μg/mL, 0.98 μg/mL, 0.0038 μg/mL 3.91 μg/mL, 0.03 μg/mL and 0.49 μg/mL respectively. Zone inhibition values in agar well diffusion test was found as 30 mm, 30 mm, 35 mm, 17 mm, 33 mm and 20 mm for S. aureus, MRSA, E. coli, K. pneumoniae, P. aeruginosa and E. faecalis, re-spectively. Conclusion: In the study, berberine-chloride anti-cancer effect on different cell lines and antibacterial activity against com-monly infectious bacteria has been shown in-vitro. © 2020 by Türkiye Klinikleri.Item Synthesis and apoptotic activities of new 2(3H)-benzoxazolone derivatives in breast cancer cells(Bentham Science Publishers, 2020) Erdag E.; Becer E.; Mulazim Y.; Vatansever H.S.; Kabadayı H.; Kesanli B.Background: 2(3H)-Benzoxazolone derivatives are preferential structural blocks in pharmacological probe designing with the possibility of modifications at various positions on the core structure. Benzoxazolones showed various biological activities such as analgesics, anti-inflammatory and anti-cancer. Objective: In the present work, we have prepared new Mannich bases of 2(3H)-benzoxazolone derivatives and evaluated their cytotoxicities and proapoptotic properties in MCF-7 breast cancer cell line. Methods: The structures of these compounds were characterized by FT-IR, elemental analysis,1H and13C NMR. Cytotoxicities of all the target compounds were investigated by MTT assay. Apoptotic properties of compounds were evaluated by immunocytochemistry using antibodies against caspase-3, cytochrome-c, FasL, and also TUNEL assay. Results: These two novel compounds, 1 and 2, both have the same piperazine substituent on the nitrogen atom of benzoxazolone and the main difference in the structures of these compounds is the presence of Cl substituent at the 5-position of the benzoxazolone ring. MTT results showed that compounds 1 and 2 were effective in terms of reduction of cell viability at 100µM and 50µM concentration for 48h, respectively. As a result of im-munohistochemical staining, Fas L and caspase-3 immunoreactivities were significantly increased in MCF-7 cells after treatment with compound 1. Additionally, caspase-3 and cytochrome-c immunoreactivities were also increased significantly in MCF-7 cells after treatment with compound 2. The number of TUNEL positive cells was significantly higher in MCF-7 cells when compared with the control group after treatment with both compounds 1 and 2. Conclusion: It could be concluded that N-substituted benzoxazolone derivatives increase potential anti-cancer effects and they could be promising novel therapeutic agents for chemotherapy. © 2021 Bentham Science Publishers.Item Relationship between IL-6 and COVID-19: To be considered during treatment(Future Medicine Ltd., 2020) Vatansever H.S.; Becer E.The detection and control of pro-inflammatory response is crucial in the early stages of viral infection. Coronavirus disease 2019 (COVID-19) is an emerging viral disease of global concern and optimal treatment has yet to be determined. Unknown response of treatment of COVID-19 is important during patient monitoring. IL-6 is one of the key cytokines after activated macrophages. Therefore, control of systemic IL-6 levels in SARS-CoV-2 infected patients may be a parameter for COVID-19 disease. This review is focused on the induction of IL-6 after viral infections as a target molecule for monitoring cellular response. © 2021 Future Medicine Ltd.Item The Effect of Resveratrol and Quercetin on Epithelial-Mesenchymal Transition in Pancreatic Cancer Stem Cell(Routledge, 2020) Hoca M.; Becer E.; Kabadayı H.; Yücecan S.; Vatansever H.S.Resveratrol and quercetin are phytochemicals that are found in a variety of plants. The aim of this study was to investigate the effect of resveratrol and quercetin on epithelial-mesenchymal transition (EMT) of CD133+ and CD133− pancreatic cancer cells. Cancer stem cells (CD133+ cells) were obtained from the PANC-1 cells by the MiniMACS system. CD133+ and CD133− PANC-1 cells were treated with different concentrations (5, 10, 25, 50, and 100 µM) of resveratrol and quercetin. Cell growth and cytotoxicity were evaluated by MTT assay. Anticancer and anti-metastatic properties of resveratrol and quercetin were determined by immunocytochemistry using antibodies (ACTA-2, IL-1β, N-cadherin, TNF-α, and vimentin). The immunostaining intensity of CD133+ cells was stronger than CD133− cells. ACTA-2, IL-1β, and N-cadherin immunoreactivities were significantly decreased, whereas TNF-α and vimentin immunoreactivities significantly increased in quercetin-treated CD133+ cells. Moreover, N-cadherin and TNF-α immunoreactivities significantly decreased in resveratrol-treated CD133+ cells. The reduction in N-cadherin and ACTA-2 immunoreactivities was higher than the increase in vimentin immunoreactivity, quercetin could prevent EMT to a greater extent than resveratrol in pancreatic cancer stem cells because of the reduced expression of N-cadherin. Quercetin could be more effective in inhibiting metastasis compared to resveratrol. © 2020, © 2019 Taylor & Francis Group, LLC.Item Cellular behavior of Colchicum troodi treated primary and metastatic colon adenocarcinoma cell lines(Mattioli 1885, 2021) Becer E.; Yavuz D.Ö.; Vatansever H.S.; Kabadayı H.; Hanoğlu A.; Meriçli F.; Meriçli A.H.Colchicum troodi belongs to Colchicaceae family that particularly rich in flavonoids, phenolic acids, tannin, fatty acids and alkaloids such as colchicine. The aim of this study was to investigate the anti-proliferative and protective effects of Colchicum troodi ethanolic extract toward relevant molecular signalling pathways on Colo-320 primer and Colo-741 metastatic colon adenocarcinoma cell lines. Colchicum troodi was collected and extracted. Different concentrations of Colchicum troodi extract were incubated for 24 h and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by MTT assays. Anticancer and antiprolifetarive activities of Colchicum troodi were investigated by immunocytochemistry using antibodies directed against to β-catenin, LGR5, jagged 1, IHH, CD133 and Ki-67 in Colo-320 and Colo-741 cells. In the MTT assay, 10 µg/ml and 5 µg/ml Colchicum troodi extract were found to be active against Colo-741 and Colo-320 cells, respectively. Colchicum troodi extract significantly increased caspase β-catenin immunoreactivities while IHH immunostaining intensity was decreased in Colo-741 cells. We conclude that Colchicum troodi extract increased β-catenin via Wnt/β-catenin pathway in Colo-741 cells. Interestingly, it decreased IHH immunoreactivities which is an antagonist of constitutive activity of Wnt/ β-catenin signaling and is assumed to play protective act during carcinogenesis. © Mattioli 1885Item Boswellia sacra essential oil manages colon cancer stem cells proliferation and apoptosis: a new perspective for cure(Bellwether Publishing, Ltd., 2021) Becer E.; Kabadayı H.; Başer K.H.C.; Vatansever H.S.Boswellia sacra Flueck essential oil is obtained from frankincense obtained from Boswellia sacra trees. The aim of this study was to investigate the in vitro anti-cancer activity of Boswellia sacra (BS) essential oil in CD133+ and CD133- Colo-320 cells. Essential oil was analyzed by GC-MS. CD133+ cells, cancer stem cells, were obtained from the Colo-320 by the MiniMACS system. Cytotoxicity were evaluated with MTT assay. Anti-cancer activity of BS essential oil was investigated by immunocytochemistry. 41 compounds were identified in the essential oil. 1:2 dilution BS essential oil was found to be active against all the cells for 24 h incubation. β-catenin immunoreactivity was significantly decreased in both BS essential oil-treated CD133+ and CD133- Colo-320 cells. Caspase-3 immunoreactivity significantly increased in BS essential oil-treated CD133- Colo-320 cells. We conclude that BS essential oil decreased β-catenin signaling molecules which play a crucial role in cancer cell proliferation. BS essential oil promoted apoptosis in CD133- Colo-320 cells. © 2020 Informa UK Limited, trading as Taylor & Francis Group.Item Resveratrol triggers Apoptosis in colon cancer cells rather than senescence(Mattioli 1885, 2021) Madencioğlu S.; Becer E.; Kabadayı H.; Vatansever H.S.; Yücecan S.Objective: Resveratrol is a phenolic compound that classified in stilbenoid and used as anticancer agent in many cancer types. The purpose of the study is to determine apoptotic and senescence inducing effects in primary (Colo-320) and metastatic (Colo-741) colon cancer cells. Methods: Cell growth and cytotoxicity were detected by MTT method in both Colo-320 and Colo-741 cell lines. Apoptotic and senescence inducing activities were tested with TUNEL staining, X-gal staining and immunocytochemistry using antibodies directed against to Bax, Bcl-2, caspase-3, Hsp27, Lamin B1, p16, cyclin B1. Results: According to MTT results, 25 μg/mL and 10 μg/mL concentrations of resveratrol were found more effective for Colo-320 and Colo-741 cell lines, respectively. As a result of immunocytochemical staining, Bax immunoreactivity was significantly increased while Bcl-2 immunoreactivity significantly decreased in Colo-320 cell line. Increased Hsp27, lamin B1 and p16 immunoreactivities on Colo-320 cells were not significant. In Colo-741 cells, Bcl-2 immunoreactivity was significantly increased. Hsp27 immunoreactivity in Colo-320 cell line was significantly higher than Colo-741 cell line. In addition, after resveratrol administration, while the TUNEL positive cells significantly increased in Colo-320 cells, X-gal positive cells was detected in Colo-741 than Colo-320 cells. Conclusion: Resveratrol can inhibit cell viability both in primer and metastatic colon cancer cells. However, resveratrol might be more effective triggering mitochondrial-mediated apoptosis in primary colon cancer cells. Apoptotic and cell cycle inhibiting effects of resveratrol may differ by cell type. Therefore, resveratrol may be a potential phytotherapeutic agent for colon cancer according to the cell origin. © Mattioli 1885.Item Fatty acid composition of Opuntia ficus-indica seed oil control angiogenic activity in colon carcinoma cell lines(Mattioli 1885, 2021) Becer E.; Kabadayi H.; Meriçli A.H.; Kivançli B.; Vatansever H.S.; Meriçli F.Opuntia ficus-indica belongs to the family Cactaceae that particularly rich in unsaturated fatty acids. The aim of this study was to investigate the composition and in vitro anti-angiogenic activity of spiny and thornless Opuntia ficus-indica seed (CPS) oils. Spiny and thornless CPS oils were obtained by supercritical CO2 extraction and analyzed by GC-MS. Cell growth and cytotoxicity were measured with MTT assay with different concentrations of spiny and thornless CPS oils in Colo-320 and Colo-741 cell lines. Anti-angiogenic activity of CPS oils were investigated by immunocytochemistry using antibodies directed against to Flt- 1, Flk-1, eNOS, iNOS, IL-6, PGE2, TNF-α and VEGF. Oleic acid (17.6%; 15.6%), linoleic acid (55.9%; 60.1%), palmitic acid (12.4%; 12.3%), elaidic acid (4%; 4.1%) were determined as the major compounds of spiny and thornless CPS oils, respectively. In the MTT assay, 1:16 dilution spiny CPS oil was found to be active against Colo-320 and Colo-741 cells for 48h incubation. Also, 1:8 and 1:16 dilutions of thornless CPS oil were more effective at inhibiting Colo-320 and Colo-741 for 48 h, respectively.We conclude that spiny CPS oil decreased signaling molecules which play in PGE2 mediated and VEGF-dependent angiogenesis. Interestingly, thornless CPS oil increased angiogenesis thought signaling molecules in colon cancer cells. © 2021 Mattioli 1885. All rights reserved.Item Comparison of the Rate of Induced Intrinsic Pathway of Apoptosis on COLO-320 and COLO-741(Springer, 2021) Hincal E.; Soykut G.; Tijjani Saad F.; Vatansever S.; Abdullahi Baba I.; Çalış İ.; Kaymakamzade B.; Becer E.Mathematical modeling is a key tool in understanding complicated behavior of large signal transduction networks. In this paper a mathematical model is developed to understand the complex signaling behavior of induced apoptosis in Colo-320 and Colo-741 cell lines. The model studies the intrinsic pathway of apoptosis induced by Apaf-1, Cyt-c, and Caspase 3. Experimentally these tests were carried out five times for three groups; namely Control, DCM and Aqueous groups. The main aim is to compare the rate of the occurrence of apoptosis in the two cells Colo-320 and Colo-741 using experimental data. Numerical results reveal that apoptosis takes place at the same time in DCM for Colo-320 and Colo-741, but slightly faster in Aqueous group for Colo-320. It was observed that it took place between the 5–7th h in all the cases. © 2021, Springer Nature Switzerland AG.Item The Role and Mechanisms of Action of Catechins in Neurodegenerative Diseases(Routledge, 2021) Özduran G.; Becer E.; Vatansever H.S.The prevalence, incidence and mortality rates of neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease are gradually increasing. New approaches are being developed to manage the progression and treatment of neurodegenerative diseases. Catechins, polyphenolic compounds, are key compounds that demonstrate therapeutic effects with their properties such as antioxidant, anti-inflammatory, anti-apoptotic properties in the prevention and treatment of neurodegenerative diseases. The therapeutic effects of catechins have been exhaustively studied in human and animal models. Catechins can have anti-inflammatory effects by suppressing inflammatory pathways and cytokines, as well as antioxidant effects such as chelating metal ions and scavenging radicals. They might reduce phosphorylation of tau proteins, aggregation of amyloid-beta and apoptotic proteins release. They can also decrease alpha-synuclein accumulation and increase dopamine levels. With all these effects, they can have an effect on neurodegenerative diseases. This review points to the potential mechanisms of catechins in neurodegenerative diseases, based on their findings in the literature review. Key teaching points Catechins can reduce amyloid-β plaque aggregation and tau phosphorylation. Catechins can decrease alfa-synuclein levels. Catechins can protect neuronal cells with their anti-apoptotic effect. More comprehensive studies are needed to clarify this issue. © 2021 American College of Nutrition.Item Neuroprotective effects of catechins in an experimental Parkinson’s disease model and SK-N-AS cells: evaluation of cell viability, anti-inflammatory and anti-apoptotic effects(Taylor and Francis Ltd., 2022) Özduran G.; Becer E.; Vatansever H.S.; Yücecan S.Objectives: The aim of the study was to establish an in vitro Parkinson’s disease (PD) model and to investigate the cell viability, anti-inflammatory, anti-apoptotic and neuroprotective effects of catechin and EGCG in SK-N-AS and in vitro PD model cells. Method: SK-N-AS human neuroblastoma cells were used. To develop an in vitro PD model, SK-N-AS cells were exposed to 6-hydroxydopamine. Model control was performed after ELISA analysis of dopamine and α-synuclein levels in the culture medium. Catechin and EGCG were administered to SK-N-AS and in vitro PD model cells. Cell viability was measured using MTT assay and trypan blue staining. Anti-inflammatory and anti-apoptotic activities of catechin and EGCG were investigated by indirect immunocytochemistry using anti-TNF-α, anti-IL-1β and anti-caspase-3. Results: After 24 hours of 6-hydroxydopamine administration at 50 μM, higher αlfa-synuclein and lower dopamine levels were found in PD model than SK-N-AS cells. Cell viability was similar between SK-N-AS and in vitro PD model cells. Treatment with both bioactive components increased cell viability of in vitro PD model cells. Caspase-3 immunoreactivity was significantly reduced in SK-N-AS and PD model cells after EGCG administration, while it was decreased only in PD model cells after catechin administration. IL-1β staining intensity weakened after catechin administration in PD model cells, after EGCG administration in SK-N-AS cells. TNF-α staining intensity was similar in both cells. Conclusion: Catechin and EGCG increased cell viability in PD model neuron cells. Both components showed anti-apoptotic and anti-inflammatory effects. Catechin may be more effective in preventing damage to neurons PD. © 2022 Informa UK Limited, trading as Taylor & Francis Group.Item Senescence-mediated anticancer effects of quercetin(Elsevier Inc., 2022) Özsoy Gökbilen S.; Becer E.; Vatansever H.S.Cellular senescence plays a key role in aging and age-related disease initiation. It is a highly dynamic and multistep process that can be stimulated by various stimuli, including cellular stress, DNA damage, telomere shortening, and oncogene activation. Also, senescence is a potent antitumor mechanism, by preventing the proliferation of cancerous cells. However, some of the senescent cells have apoptosis resistance and can cause recurrence in cancer. A new class of drugs termed senolytics selectively kill and eliminate senescent cells. In recent years, natural compounds such as quercetin have been discovered to be effective as senolytic agents. Quercetin is a phytochemical that has strong antioxidant properties and pro-apoptotic effects and has been investigated for many years. Additionally, it has great potential to be used as a senolytic agent. According to preclinical and early-phase clinical data of senolytic agent research, quercetin administration appears to be effective in preventing or alleviating cancer formation. In this paper, we review the importance of cellular senescence in carcinogenesis and the effects of quercetin on senescence, as well as quercetin's potential effects as a pro-apoptotic agent and suppressor of cancer cell proliferation. © 2022 Elsevier Inc.Item Cytotoxic activity and antioxidant effects of Origanum onites essential oil and its two major contents, carvacrol and p-cymene on human colorectal (HCT116) and hepatocelluler carcinoma (HepG2) cell lines(Taylor and Francis Ltd., 2022) Becer E.; Mutlu Altundag E.; Başer K.H.C.; Vatansever H.S.Origanum onites L. (O. onites) is one of the cultivated Origanum species in Turkey. The aim of this study was to determine the cytotoxicity and cellular antioxidant effects of O. onites essential oil, carvacrol and p-cymene on HCT116 and HepG2 cells. O. onites essential oil components were analyzed by GC/MS and GC/FID. Cytotoxicity was analyzed via the MTT assay in HCT116 and HepG2 cells. Cellular antioxidant activities of O. onites essential oil, carvacrol and p-cymene were determined with DCFH-DA method. GC/MS analysis identified carvacrol (%78.4) as the main constituent of O. onites essential oil. 400 µg/ml concentration of O. onites essential oil, carvacrol and p-cymene were found to be active against HCT116 and HepG2 cells. Also, carvacrol showed higher cellular antioxidant activity than O. onites essential oil and p-cymene. We conclude that O. onites essential oil and carvacrol may be a candidate for pharmaceutical products in treatment of cancer. © 2022 Informa UK Limited, trading as Taylor & Francis Group.