Browsing by Author "Becerik, S"
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Item An investigation on the seasonal variations of the biomarkers of oxidative stress response and their correlations to Polonium-210 in mussel (Mytilus galloprovincialis) and common sole (Solea solea) from Izmir Bay, TurkeyAslan, E; Görgün, AU; Katalay, S; Filizok, I; Becerik, S; Aydemir, TIt is well known that the marine organisms are used as biological indicators for environmental pollution studies. Among these studies, the research on oxidative stress has been increasing in recent years. In this study, mussels (Mytilus galloprovincialis) and fish (Solea solea) samples were collected seasonally from inciralti, Izmir, Turkey. This station was in an area where fishing is carried out for human consumption. The relationship between Po-210 and oxidative stress markers (lipid peroxidation (LPO), H2O2 and proline) was investigated in the mussel tissue (digestive gland, gills) and fish tissue (liver, gills) samples. The present study indicated that H2O2 accumulated with increasing Po-210 concentration in mussel samples. Statistically significant correlation were found between H2O2 and Po-210 and LPO and proline in mussel samples. This correlation between LPO and proline can be attributed to common environmental parameters (other than Po-210) affecting expression of both LPO and proline levels. There was not a significant correlation between Po-210 and LPO levels. Similarly, a significant correlation was not found between Po-210 and proline.Item Gingival Crevicular Fluid and Plasma Acute-Phase Cytokine Levels in Different Periodontal DiseasesBecerik, S; Öztürk, VÖ; Atmaca, H; Atilla, G; Emingil, GBackground: The aim of the present study is to investigate gingival crevicular fluid (GCF) and plasma acute-phase cytokines, interleukin-I beta (IL-1 beta), interleukin-6 (IL-6), interleukin-11 (IL-11), oncostatin M (OSM), and leukemia inhibitory factor (LIF) levels in patients with different periodontal diseases. Methods: Eighty individuals were included in this study; 20 with chronic periodontitis (CP), 20 with generalized aggressive periodontitis (GAgP), 20 with gingivitis, and 20 classified as healthy (H). Probing depth, clinical attachment level, plaque index, and papilla bleeding index were recorded. Plasma and GCF IL-1 beta, IL-6, IL-11, OSM, and LIF levels were analyzed by enzyme-linked immunosorbent assay. Results: CP and GAgP groups had significantly higher GCF IL-1 beta, IL-6, and IL-11 levels when compared with the H group (P <0.05). Conversely, GCF LIF levels of the CP and GAgP groups were lower than those of the H group (P<0.05). GCF OSM levels did not differ significantly among study groups. Plasma levels of all the cytokines studied were not significantly different among the study groups. Conclusions: Based on the present data, elevated IL-1 beta, IL-6, and IL-11 GCF levels, but not plasma levels, are suggested as reliable inflammatory biomarkers in periodontal diseases. Decreased LIF levels in diseased groups might reflect the possible beneficial effects of LIF in the modulation of inflammatory response in gingiva. J Periodontol 2012;83: 1304-1313.Item Gingival crevicular fluid calprotectin, osteocalcin and cross-linked N-terminal telopeptid levels in health and different periodontal diseasesBecerik, S; Afacan, B; Öztürk, VÖ; Atmaca, H; Emingil, GAim: The aim of the present study was to investigate gingival crevicular fluid (GCF) calprotectin, osteocalcin and cross-linked N-terminal telopeptide (NTx) levels in health along with different periodontal diseases. Material and methods: Twenty chronic periodontitis (CP), 20 generalized aggressive periodontitis (G-AgP), 20 gingivitis and 20 healthy subjects were included. Probing depth, clinical attachment level, plaque index and papillary bleeding index was recorded. GCF calprotectin, osteocalcin and NTx levels were analyzed by enzyme-linked immunosorbent assay (ELISA). Results: CP, G-AgP and gingivitis groups had higher GCF calprotectin total amount compared to healthy subjects (p < 0.008). CP and G-AgP groups had similar, but higher levels compared to gingivitis groups (p < 0.008). CP and G-AgP groups had lower GCF osteocalcin total amount compared to gingivitis and healthy groups (p < 0.008). CP group had higher GCF NTx but lower osteocalcin total amount and osteocalcin/NTx ratio than the G-AgP group (p < 0.008). Conclusions: Our results suggest that elevated GCF calprotectin levels play a role as a reliable inflammatory marker in the pathogenesis of periodontal disease. Fluctuating GCF levels of osteocalcin and NTx might point out to the abnormal bone turnover in periodontitis. Our data document for the first time the role of NTx in the pathogenesis of different periodontal diseases.Item Removal of nickel(II) ions by histidine modified chitosan beadsEser, A; Tirtom, VN; Aydemir, T; Becerik, S; Dinçer, AIn order to increase the nickel adsorption capacity of raw chitosan beads (CB), they were chemically modified with histidine (HIS-ECH-CB) by using crosslinking agent, epichlorohydrine (ECH). The nature and morphology of the sorbent were characterized using FTIR, TGA and SEM analysis. For optimization of adsorption conditions, sorption experiments were performed by varying contact time, pH, temperature and initial nickel concentration. Based on the adsorption experiment, the HIS-ECH-CB showed the significant adsorption capacity of 55.6 mg/g under the optimal adsorption condition. Nickel adsorption isotherms data were fitted to Freundlich isotherm. Thermodynamic parameters namely Delta G degrees. Delta H degrees and Delta S degrees of the Ni(II) adsorption process were calculated. The negative values of Gibbs free energy of adsorption (Delta G degrees) indicated the spontaneity of the adsorption of Ni(II) ions on the histidine modified chitosan. Desorption of Ni(II) ions from HIS-ECH-CB could be done rapidly by using 0.1 M HCl, HNO3 and EDTA solutions and the beads could be used again to adsorb Ni(II) ions. (C) 2012 Elsevier B.V. All rights reserved.Item Removal of erythrosine dye from aqueous solutions using magnetic chitosan with erythrosine as imprinted moleculesEser, A; Aydemir, T; Becerik, S; Dinçer, AA novel, chitosan coating on the surface of magnetite (Fe3O4) (erythrosine-imprinted magnetic chitosan (EIMC)) was successfully synthesized using erythrosine (ER) as a template for adsorption and the removal of ER from aqueous solutions. Characterization of the obtained EIMC was achieved by FTIR spectra, SEM micrographs, and TGA analysis. Batch adsorption experiments of EIMC and non-imprinted magnetic chitosan (NIMC) were performed to investigate the adsorption conditions. The results showed that the maximum adsorption capacity for EIMC and NIMC was observed at pH 6 and temperature 40 degrees C. Equilibrium adsorption was achieved within 3 h. Adsorption process could be well described by Freundlich adsorption isotherms. Thermodynamic parameters namely Delta G degrees, Delta H degrees, and Delta S degrees of the ER adsorption process were calculated. The negative values of Gibbs free energy of adsorption (Delta G degrees) indicated the spontaneity of the adsorption of ER dye on the EIMC and NIMC. Desorption of ER from EIMC and NIMC could be done rapidly using 0.1 M NaOH solution and the beads could be used again to remove ER. Results show that imprinting technique increases the removal amount of ER dye from aqueous solutions.Item Removal of lead (II) ions from aqueous solution by using crosslinked chitosan-clay beadsTirtom, VN; Dinçer, A; Becerik, S; Aydemir, T; Çelik, AA simple and effective biodegradable material known as chitosan-clay composite beads were prepared to remove Pb(II) ions from aqueous solution. For this purpose, various important parameters such as contact time, pH and temperature were examined on the adsorption of Pb(II) ions onto crosslinked chitosan-clay composite beads. Maximum adsorption capacity of Pb(II) was observed at pH 4.5 and 25 degrees C and calculated as 7.93 mg/g according to Langmuir isotherm model. Thermodynamic parameters namely Delta G degrees, Delta H degrees and Delta S degrees of the Pb(II) adsorption process have been calculated as 7.889 kJ/mol, -15.131 kJ/mol and -0.0785 kJ/molK respectively. EDTA was the best eluent for the desorption of Pb(II) ions from the crosslinked chitosan-clay beads. Scanning electron microscope (SEM) was used to characterize the surface morphology of the crosslinked chitosan-clay beads.Item Poly(methyl methacrylate-ethylene glycol dimethacrylate) copolymer for adsorptive removal of erythrosine dye from aqueous solutionAltindag, IG; Dinçer, A; Becerik, S; Eser, A; Aydemir, TThe preparation and characterization of poly(methyl methacrylate-ethylene glycol dimethacrylate), (poly(MMA-EGDMA)) polymer for erythrosine adsorption has been investigated. Erythrosine is a synthetic red dye used to color food. Water-soluble acid dyes have caused serious water pollution. Poly(MMA-EGDMA) polymer showed better adsorption performance for erythrosine at acidic region and at 30 degrees C. The adsorption process had also been verified by Langmuir and Freundlich adsorption isotherms at 30, 40, and 50 degrees C. Free energy of adsorption ( increment G degrees), enthalpy ( increment H degrees), and entropy ( increment S degrees) changes were calculated to predict the nature of adsorption. The estimated value for increment G degrees was calculated as -125.6 kJ/mol at 303 K (30 degrees C). The estimated value for increment H degrees was found as -60.69 kJ/mol at 303 K. The negative value for increment H degrees indicated that the adsorption of erythrosine on poly(MMA-EGDMA) polymer was an exothermic process.Item Immobilization of tyrosinase on chitosan-clay composite beadsDinçer, A; Becerik, S; Aydemir, TTyrosinase was immobilized on glutaraldehyde crosslinked chitosan-clay composite beads and used for phenol removal. Immobilization yield, loading efficiency and activity of tyrosinase immobilized beads were found as 67%, 25% and 1400 U/g beads respectively. Optimum pH of the free and immobilized enzyme was found as pH 7.0. Optimum temperature of the free and immobilized enzyme was determined as 25-30 degrees C and 25 degrees C respectively. The kinetic parameters of free and immobilized tyrosinase were calculated using L-catechol as a substrate and K-m value for free and immobilized tyrosinase were found as 0.93 mM and 1.7 mM respectively. After seven times of repeated tests, each over 150 min, the efficiency of phenol removal using same immobilized tyrosinase beads were decreased to 43%. (C) 2011 Elsevier B.V. All rights reserved.Item Comparative adsorption of Ni(II) and Cd(II) ions on epichlorohydrin crosslinked chitosan-clay composite beads in aqueous solutionTirtom, VN; Dinçer, A; Becerik, S; Aydemir, T; Çelik, ARemoval of Ni(II) and Cd(II) by adsorption on epichlorohydrin crosslinked chitosan-clay composite beads was examined in solutions representative of contaminated solutions containing heavy metals. Several important parameters influencing the adsorption of Ni(II) and Cd(II) ions such as contact time, pH, temperature and effect of metal concentration were investigated systematically by batch experiments. Langmuir and Freundlich adsorption models were used to describe adsorption isotherms and constants. The obtained results showed that the equilibrium adsorption behavior of Ni (II) and Cd (II) on epichlorohydrin crosslinked chitosan-clay composite beads could be applied to Langmuir and Freundlich models respectively. Maximum adsorption capacities for Ni(II) and Cd(II) ions were found as 32.36 mg g(-1) and 72.31 mg g(-1) respectively. The recovery of the metal ions after adsorption and the regeneration of the adsorbent was carried out by treatment of the loaded beads with either 0.1 M HNO3, or 0.01 M EDTA. The adsorbents were characterized by FTIR, SEM and TGA analysis. (C) 2012 Elsevier B.V. All rights reserved.Item PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS FROM OCIMUM BASILICUM, APIUM GRAVEOLENS AND LEPIDIUM SATIVUM SEEDSAydemir, T; Becerik, SThe antioxidative properties and total phenolic contents of methanol, ethanol and water extracts from Ocimum basilicum, Apium graveolens and Lepidium sativum seeds were investigated and the results were compared with standards. The methanol extracts of the seeds had significantly higher (P < 0.05) superoxide radical scavenging activity with the concentration for 50% inhibition (IC50) value of 98.73 mu g/mL for O. basilicum, 121.57 mu g/mL for A. graveolens and 166.16 mu g/mL for L. sativum. The results indicated that all the seed extracts showed excellent H2O2 scavenging activities. IC50 values for H2O2 scavenging activity by methanol extract of O. basilicum, A. graveolens, L. sativum and butylated hydroxyanisole were found to be as 49.9, 52.3, 65.2 and 49.6 mu g/mL, respectively. O. basilicum methanol extract had significantly (P < 0.05) higher 1,1-diphenyl-2-picryl-hydrazyl scavenging effect than the other two seeds. The methanol extracts of three seeds were better in Fe+2 chelating activity, reducing power and higher in the content of total phenol as compared with ethanol and water extracts. PRACTICAL APPLICATIONS Recent epidemiological studies have strongly suggested that consumption of certain plant materials such as seeds, leaves, fruits and roots may reduce the risk of chronic diseases related to oxidative stress on account of their antioxidant activity and promote general health benefits. In this study, all seeds extracts exhibited good antioxidant activity (78.3-96.9%) at concentration 500 mu g/mL. Total phenolic content in the seed extracts ranged from 51 to 92 mg GAE/100 g extract. In addition, there was a statistically significant correlation between the amount of phenolic compounds and antioxidant activity (R2 = 0.9058, P < 0.05) in all the seed extracts. Therefore, O. basilicum, A. greveolens and L. sativum seed extracts have the potential to be developed into dietary supplements and nutraceuticals.Item Gingival Crevicular Fluid Osteocalcin, N-Terminal Telopeptides, and Calprotectin Levels in Cyclosporin A-Induced Gingival OvergrowthBecerik, S; Gürkan, A; Afacan, B; Özturk, VÖ; Atmaca, H; Töz, H; Atilla, G; Emingil, GBackground: The aim of this cross-sectional study is to investigate gingival crevicular fluid (GCF) osteocalcin, crosslinked N-terminal telopeptide (NTx), and calprotectin levels in cyclosporin A (CsA)-induced gingival overgrowth (GO). Methods: Forty medicated patients with CsA including 20 with GO (CsA GO+), 10 without GO (CsA GO-), 10 with GO and chronic periodontitis (CsA CP) and 60 patients with CP alone, 20 patients with gingivitis, and 20 healthy patients were enrolled. Probing depth, clinical attachment level, plaque index, and papillary bleeding index were recorded. GCF calprotectin, osteocalcin, and NTx levels were analyzed by enzyme-linked immunosorbent assay. Parametric tests were used for statistical analysis. Results: The CsA GO+ and CP groups had significantly lower GCF osteocalcin levels and osteocalcin/NTx ratio than the healthy group, whereas GCF osteocalcin levels and osteocalcin/NTx ratio in the gingivitis group were higher than the CsA GO+, CsA GO-, CsA CP, and CP groups (P < 0.05). The CP group had elevated GCF calprotectin levels compared to the other study groups (P < 0.05). The CsA GO+ and CsA GO-groups also had higher GCF calprotectin levels compared to the CsA CP, gingivitis, and healthy groups (P < 0.05). Conclusions: Increased GCF calprotectin and decreased GCF osteocalcin levels in the CsA GO+ and CsA GO-groups might suggest that CsA plays a role on the levels of these markers. The similarity of GCF osteocalcin, NTx, and calprotectin levels in the CsA GO+ and CsA GO-groups might suggest that these molecules are not involved in the pathogenesis of GO. J Periodontol 2011; 82: 1490-1497.Item Interleukin-6 Family of Cytokines in Crevicular Fluid of Renal Transplant Recipients With and Without Cyclosporine A-Induced Gingival OvergrowthGürkan, A; Becerik, S; Öztürk, VÖ; Atmaca, H; Atilla, G; Emingil, GBackground: Interleukin (IL)-6 family of cytokines, including IL-6, oncostatin M (OSM), leukemia inhibitory factor (LIF), and IL-11, have fibrogenic features. The current study determines gingival crevicular fluid (GCF) levels of fibrosis-related IL-6-type cytokines in cyclosporine A (CsA)-induced gingival overgrowth (GO). Methods: Eighty non-smokers were included (40 CsA-medicated renal transplant patients with GO [GO+; n = 20] or without GO [GO-; n = 20], 20 individuals with gingivitis, and 20 healthy participants). Probing depth and plaque, papilla bleeding, and hyperplastic index scores were recorded. GCF samples were obtained from the mesio-buccal aspects of two teeth. GCF IL-6, IL-1 beta, OSM, LIF, and IL-11 levels were analyzed by enzyme-linked immunosorbent assay. Results: The GO+ and GO- groups had higher IL-6 total amounts than the healthy group (P < 0.008). IL-1 beta total amounts in the GO+ group were significantly higher than in both the healthy and GO- groups (P < 0.008). OSM total amount was elevated in the GO+ and GO- groups compared with both the gingivitis and healthy groups (P < 0.008). All groups had similar LIF and IL-11 total amounts (P > 0.008). Moderate positive correlations were detected among IL-6, IL-1 beta, OSM, and IL-11 total amount in GCF and clinical parameters (P < 0.05). Conclusions: IL-6 and OSM increases in GCF as a result of CsA usage or an immunosuppressed state irrespective of the severity of inflammation and the presence of GO. The IL-6 family of cytokines might not be directly involved in biologic mechanisms associated with CsA-induced GO. Lack of an association between assessed IL-6 cytokines and CsA-induced GO might indicate distinct effects of these cytokines on fibrotic changes of different tissues.