Browsing by Author "Boyacioglu M."
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Item Embryotoxic effects of water and sediment from nif brook (Western, Turkey) on sea urchin paracentrotus lividus(2009) Arslan O.C.; Parlak H.; Katalay S.; Boyacioglu M.; Karaslan M.A.In this investigation embryo toxicity of water and sediment samples collected from Nif Brook of Izmir, Turkey has been studied. Five sampling sites from the brook were selected. Sea urchin embryos were utilized for evaluating the toxicity of water and sediment. The effects on developing embryos were evaluated by scoring developmental defects. The water and sediment samples were tested with 0.001, 0.01, 0.1 dilutions in natural sea water. All water and sediment samples of the brook were assessed to be toxic according to the sea urchin embryotoxicity results. The embryotoxicity of water samples concentration-dependent and significant growth reduction at the early life stages and an increase in larval malformations as skeleton deformities at the pluteus stage were observed (approximately 59% in 0.001 ml/ml from station 5). The most polluted sediment samples displayed a dramatic embryotoxicity, up to approximately 98% developmental arrest in embryos reared in 0.1 g/ml of sediment from station 5. © by PSP Volume 18-No 5a. 2009.Item Detecting micronuclei frequency in some aquatic organisms for monitoring pollution of Izmir Bay (Western Turkey)(2010) Çakal Arslan Ö.; Parlak H.; Katalay S.; Boyacioglu M.; Karaaslan M.A.; Guner H.Micronuclei tests is a system of mutagenicity testing used for determining the pollution and chemicals causing changes in DNA fragments such as micronuclei in the cytoplasm of interphase cells. Damage caused on the DNA by genotoxic pollutants is the first consequence occurring in the aquatic organisms. Thus, it was attempted to determine whether pollution affected the erythrocytes and gills of fish Gobius niger and haemolymph and gills of mussels Mytilus galloprovincialis living in Izmir Bay at the level of DNA by the means of micronuclei (MN) test. Organisms used in the MN test were collected from seven locations (Alsancak, Alaybey Shipyard, Karsiyaka, Bostanli, Göztepe, Konak and Pasaport) which are known as the most polluted part of inner Bay of Izmir (Western Coast of Turkey). According to the results of the present study, frequency of MN was found at high level in Alaybey Shipyard and Pasaport where wastes from existing dockyard contributed to high level of pollution. In conclusion, this study indicates that the micronuclei test gives sensitive results in monitoring the pollution, especially the pollution of harbor, and thus it might be used as standard method in regular monitoring of pollution of coastal ecosystem. © 2009 Springer Science+Business Media B.V.Item Study of mutagenicity of metabolized pollutants in some tissues of black gobby (Gobius niger) and black mussels (Mytilus galloprovincialis) from izmir bay (Western Turkey)(2011) Boyacioglu M.; Parlak H.; Arslan O.C.; Katalay S.; Karaslan M.A.In the present study, fish and mussel samples from Izmir bay of Agean sea were studied for their mutagenic potential in TA98 and TAIOO strains of Salmonella typhimurium using Ames test (plate incorporation assay) without metabolic activation. Black gabby (Gobius niger) samples were collected from Bostanli and Pas aport locations and mussel (Mytilus galloprovincialis) samples were collected from Alsancak, Alsancak harbour, Alaybey Shipyard, Karsiyaka, Bostanli, Goztepe, Konak and Pasaport locations on Izmir bay. Extracts were obtained from hepatic and muscular tissues of black goby and whole soft parts of the mussels. Obtained extracts were studied for mutagenicity using the Ames test with S. typhimurium TA98 and TAIOO strains. According to results of the present study, mutagenicity was observed in liver extracts of the fish from Pasaport location whereas no mutagenicity was observed in liver and muscle extracts of fish from Bostanli location. Among the extracts of mussel samples collected from 8 locations only those from Alsancak Harbour were observed to possess weakly and directly mutagenic effects on S. typhimurium TA98 (frame shift mutations). © Medwell Journals, 2011.Item Phytotoxicity of water and sediment from Nif Brook (Izmir, Turkey) on green algae desmodesmus (=Scenedesmus) subspicatus; [Nif Çayı (İzmir, Türkiye) su ve sedimenti'nin yeşil alg desmodesmus (=Scenedesmus) subspicatus üzerine fitotoksisitesi](Cevkor Vakfi, 2012) Katalay S.; Boyacioglu M.; Cakal Arslan O.; Parlak H.; Ali Karaaslan M.The water of Nif Brook (Izmir, Turkey) is polluted by industrial, domestic, and agricultural sources. As the water of the brook is used for domestic and industrial water supply as well as for irrigation, it is of great importance to know the toxicity potential for the natural populations. For this purpose the standard test protocol for the short term phytotoxicity test method OECD 201 Algal Growth Inhibition has been evaluated with cultures of green algae Desmodesmus (=Scenedesmus) subspicatus as the representative of the first trophic level. The test has been used to assess the toxicity of both water and sediment samples from the brook. Five sampling sites from the brook were selected considering the type of pollution sources. The water samples were tested with 10, 20, 40, 60, 80 and 100 % dilutions in a test medium. Extracts of sediment samples were assayed in three different concentrations (20, 40 and 100 mg/mL). The effects on the growth of D. subspicatus were evaluated by scoring cell numbers under the light microscope with a Neubauer haemocytometer counting chamber. According to the results, the water samples stimulated the algal growth (except station 1); although all sediment samples inhibited the growth of populations, in several grades.Item Mutagenicity of sediment samples from Nif brook (Western Turkey)(Medwell Journals, 2012) Boyacioglu M.; Parlak H.; Arslan O.C.; Katalay S.; Karaslan M.A.The present study aimed to assess mutagenicity of the sediment samples from Nif brook using solvent extraction method and to compare its results with those of a previous study on mutagenicity using Soxhlet-extraction method. Sediment samples were collected from 5 sites located in Nif brook in April, 2007. Sediment samples from 5 sites on Nif brook were studied for their mutagenic potential on TA 98 and 100 strains of Salmonella typhimurium using Ames test (Plate incorporation assay) without metabolic activation. Extracts of the sediment samples were assayed in three different concentrations (In 25, 50, 100 ?g). Sediment sample from Nif 5 sides was observed to be weak-mutagenic and those from Nif 1 and 4 sides were observed to have toxic effects. Toxic effects of the sediment samples on bacteria were observed on the basis of significantly reduced numbers of revertants compared to the solvent controls. According to Ames criteria, sediment samples from the Nif 5 were considered as mutagenic on TA 98 strain. According to the present study, mutagenicity was detected in Nif brook by solvent extraction method. © Medwell Journals, 2012.Item Changes in the glutathione-s transferase activity of the mussel Mytilus galloprovincialis during exposure to bisphenol-A(Parlar Scientific Publications, 2014) Arslan O.C.; Parlak H.; Boyacioglu M.; Karaslan M.A.; Katalay S.Bisphenol A (BPA), is one of the most important industrial chemicals synthesized for diverse applications. The environmental concentrations of BPA are at high risk level due to use widely in many fields of industry according to the latest studies. In our study mussels (Mytilus galloprovincialis) were exposed to 50 μg/L of BPA and the changes of hepatic biomarker glutathione-s transferase (GST) activity were investigated. The results showed that the activity of GST was increased in BPA group (20.44 %) compared with control. Based on this experiment, we recommended that GST might be used as a biomarker of environmental pollution. © by PSP.