Browsing by Author "Boyacioglu S.O."

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    Never in mitosis gene A-related kinase 6 and aurora kinase A: New gene biomarkers in the conversion from ulcerative colitis to colorectal cancer
    (Spandidos Publications, 2015) Gerçeker E.; Boyacioglu S.O.; Kasap E.; Baykan A.; Yuceyar H.; Yildirim H.; Ayhan S.; Ellidokuz E.; Korkmaz M.
    Ulcerative colitis (UC) is an important risk factor for colorectal cancer (CRC). Histone modifications are one of the epigenetic mechanisms that may have key roles in the carcinogenesis of CRC. At present, there are no studies comparing histone modification patterns of UC and CRC in the literature. Therefore the aim of the present study was to investigate whether genes, particularly those involved in histone modification, have value in patient monitoring with regards to CRC development in UC. Key gene expressions of the histone modification enzyme were assessed and compared in CRC, UC and control groups using the RT-PCR array technique. Patients were divided into subgroups based on the extent and duration of the disease and inflammatory burden, which are considered risk factors for CRC development in UC patients. In UC and CRC groups, a significantly higher overexpression of the NEK6 and AURKA genes compared to the control group was identified. In addition, there was a significantly higher overexpression of HDAC1 and PAK1 genes in the UC group, and of HDAC1, HDAC7, PAK1 and AURKB genes in the CRC group. NEK6, AURKA, HDAC1 and PAK1 were significantly overexpressed in patients with a longer UC duration. Overexpression of AURKA and NEK6 genes was significantly more pronounced in UC patients with more extensive colon involvement. HDAC1, HDAC7, PAK1, NEK6, AURKA and AURKB are important diagnostic and prognostic markers involved in the carcinogenesis of CRC. HDAC1, PAK1, NEK6 and AURKA may be considered as diagnostic markers to be used in CRC screening for UC patients.
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    Alteration in methylation pattern of retinoblastoma 1 gene promotor region in intestinal metaplasia with or without helicobacter pylori and gastric cancer patients
    (Wroclaw University of Medicine, 2016) Boyacioglu S.O.; Kasap E.; Yuceyar H.; Korkmaz M.
    Background. Helicobacter pylori, intestinal metaplasia (IM), and gene methylation play important roles in gastric carcinogenesis. However, the association among H. pylori infection, IM, gastric cancer (GC), and gene methylation is not fully understood. Cell cycle control involving retinoblastoma 1 (RB1) gene is one of the main regulatory pathways reported to be altered in gastric carcinogenesis. Objectives. The purpose of this research is to assess the methylation status of RB1 gene in GC and IM with or without H. pylori infection, and to discuss the possible role of H. pylori-induced RB1 gene methylation in the mechanism of gastric carcinogenesis. Material and Methods. The methylation profile of RB1 gene was analyzed by sodium bisulfite modification and methylation-specific PCR in GC (n = 24), IM patients with H. pylori positive (n = 20) and negative (n = 20), and control subjects (n = 20). Results. According to methylation levels in RB1 gene; the high correlation values were detected between H. pylori positive-IM group and GC group, and between H. pylori positive-IM and H. pylori negative-IM groups (p < 0.05). No correlations between H. pylori negative-IM and GC groups and between GC and control groups were detected in methylation status of RB1 gene. Conclusions. High methylation levels in RB1 gene in H. pylori positive individuals may suggest an elevated risk of gastric cancer occurrence. © Copyright by Wroclaw Medical University.
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    ASSOCIATION OF MICRO RNA EXPRESSIONS WITH PEDIATRIC CELIAC CLINICAL FINDINGS; [ASOCIJACIJA EKSPRESIJE MIKRO RNK SA CELIJAKIČNIM NALAZOM DECE]
    (Serbian Genetics Society, 2023) Dogan G.; Boyacioglu S.O.; Caliskan M.; Kasap E.; Ayhan S.; Kasirga E.
    There is a need to determine the relationship between the function of the immune system and miRNA expression in pediatric celiac disease (pCD). We aimed to describe the expression profiles of miRNAs in Turkish pCD patients based on the clinical and pathological findings. This study was conducted on 33 pCD patients and 33 pediatric control subjects with normal biopsy results. Four most common mutations (DQA1*05, DQB1*02, DQA1*03, DQB1*03:0.2) on HLA gene in pCD were screened. Paraffin-embedded biopsy tissue samples were used in miRNA isolations followed by cDNA synthesis. Expression of miRNAs were evaluated in the groups with qRT-PCR array-method. Significant underexpression of hsa-miR-194-5p gene was detected in pCD patients compared to the control group. The hsa-miR-194-5p gene was significantly underexpressed in anemic or short stature pCD patients compared to the control. The genes of hsa-miR-29b-3p, hsa-miR-30e-5p, and hsa-miR-146a-5p were significantly overexpressed in the patients with constipated celiac patients. Significant overexpression of hsa-miR146a-5p gene was detected in the Marsh2 and Marsh3a groups. The hsa-miR-29b-3p, hsa-miR-30e-5p, hsa-let-7a-5p, hsa-miR-27a-3p, hsa-miR141-3p, hsa-miR143-3p, and hsa-miR-146a-5p miRNA genes were significantly overexpressed in the Marsh3b group. Also, the hsa-miR-194-5p and hsa-miR-26a-5p genes were significantly underexpressed in the comparison of Marsh3c group to the control. These results suggest that miRNA expressions are likely to play a role in the pathogenesis of pCD. It is believed that the current results present valuable inferences that may help understand the genetic boundaries on pCD, which might be further supported by follow up studies on other miRNAs © 2023, Genetika.All Rights Reserved.