Browsing by Author "Buyukkececi F."
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Item Augmentation of methylprednisolone-induced differentiation of myeloid leukemia cells by serine/threonine protein phosphatase inhibitors(1999) Uzunoglu S.; Uslu R.; Tobu M.; Saydam G.; Terzioglu E.; Buyukkececi F.; Omay S.B.To elucidate the roles of serine/threonine protein phosphatases type 1 (PP1) and type 2A (PP2A) in methylprednisolone-induced differentiation of HL60 cells into granulocytes and K562 cells into monocytes, we examined the effect of serine/threonine protein phosphatase inhibitors, okadaic acid and Cal-A on the proliferation/differentiation of HL60 and K562 cells. Okadaic acid and Cal-A augmented methylprednisolone induced granulocytic differentiation and cell death of HL60 cells and monocytic differentiation and cell death of K562 cells in different dose ranges, respectively. These data suggest an important role of PP1 and PP2A in the mechanism leading to differentiation of leukemic cells.Item Up-regulation of serine/threonine protein phosphatase type 2A regulatory subunits during methylprednisolone-induced differentiation of leukaemic HL-60 cells(2000) Aydin H.H.; Selvi N.; Saydam G.; Tobu M.; Uzunoglu S.; Uslu R.; Buyukkececi F.; Omay S.B.Serine/threonine protein phosphatase 2A (PP2A) may play a role in leukaemic cell differentiation of the HL 60 myeloid leukaemic cell-line after methylprednisolone induction. We have investigated the specific enzyme activity and expression of catalytic and regulatory subunits of PP2A. The resulting specific enzyme activity and immunoblots showed an increase in enzyme activity and the expression of regulatory subunits after methylprednisolone treatment. There was no change in the expression of PP2A catalytic subunits. It is suggested that the effect of methylprednisolone on leukaemic differentiation may be the result of PP2 A upregulation.Item Sclerosing extramedullary hematopoietic tumor mimicking intra-abdominal sarcoma(Seoul National University, 2015) Karaarslan S.; Nese N.; Oncel G.; Ozsan N.; Akalin T.; Kaplan H.; Buyukkececi F.; Hekimgil M.[No abstract available]