Browsing by Author "Cal, C"

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    Zoledronic Acid Effects Interleukin-6 Expression in Hormone-Independent Prostate Cancer Cell Lines
    Asbagh, LA; Uzunoglu, S; Cal, C
    Objective: To investigate the inhibitory effects of zoledronic acid (ZA) on tumor related growth factor IL-6 in hormone resistant prostate cancer cell lines. The association between apoptosis and IL-6 inhibition was also assessed. Materials and Methods: PC-3 and DU145 cell lines were treated with different concentrations of ZA (1-100 mu M) at various intervals (24-72 h.). The cell viability was investigated by XTT assay and apoptotic effect was evaluated by cell death detection ELISA kit. Caspase 3/7 activity assay was performed to confirm apoptosis. IL-6 levels were measured by ELISA in the supernatant, and these data were also confirmed by IL-6 mRNA analysis using RT-PCR. Results: PC-3 and DU145 cell lines were sensitive to ZA mediated cytotoxicity in a dose- and time-dependent manner. However, the apoptotic effect was significantly different among PC-3 and DU145 cells (p < 0.05). IL-6 secretion was significantly lower in both cell lines, compared to the untreated control cells (p < 0.05). Although the increased inhibition of IL-6 secretion was associated with increased apoptosis in DU 145 cells (p = 0.002), there was no similar association for PC-3 cell line (p = 0.347). When compared to the untreated controls, the number of cDNA copies was significantly lower in the ZA treated DU145 cell line at doses of 30 and 90 mu M (p < 0.05), suggesting a reduced expression of IL-6 mRNA. Conclusion: ZA exhibited a time- and dose-dependent apoptotic effect on PC-3 and DU 145 prostate cancer cell lines and this effect was associated with inhibited secretion of IL-6 in DU145 cell line.
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    A SURVEY OF PATIENT PREPARATION AND TECHNIQUE OF ULTRASOUND-GUIDED PROSTATE BIOPSY: A MULTICENTER STUDY OF UROONCOLOGICAL ASSOCIATION
    Bozlu, M; Akduman, B; Mungan, U; Özen, H; Baltaci, S; Türkeri, L; Kirkali, Z; Akdas, A; Adsan, O; Akdogan, B; Altinel, M; Ataus, S; Ayan, S; Bilen, CY; Çal, Ç; Cal, C; Dündar, M; Iseri, C; Kosan, M; Lekili, M; Müezzinoglu, T; Özer, G; Ozgok, Y; Perk, H; Soyupak, B; Soyupek, S; Soylu, A; Sözen, S; Sengör, F; Tansug, Z; Tekin, A; Yildirim, A
    Introduction: Ultrasound-guided prostate biopsy is the standard method for the diagnosis of prostate cancer. The aim of the present survey is to assess the variability in patient preparation and technique of ultrasound-guided prostate biopsy among Turkish Urologists. Materials and Methods: In July 2004, a questionnaire was sent out to e-mail addresses of the members of Urooncological Association, asking about the details of prostate biopsy protocol of the members. The survey consisted of multiple choice questions about the patient preparation and prostate biopsy technique. Responses were acquired via e-mail and analyzed in detail. Results: Thirty two urologists from 24 centers responded. The biopsy procedure was performed by the urologist only in 54.16% of the centers, both urologist and radiologist in 37.5%, and radiologist only in 8.33%. Transrectal route was the most common method for ultrasound-guided prostate biopsy. A half of the responders performed biopsy when PSA was greater than 4 ng/ml. All of the centers administered antibiotic and a half of them used enema before the procedure. Approximately 37% of responders did not administer any type of analgesia, but 29.1% of all responders administered a periprostatic nerve block for reducing pain during the procedure. Most urologists obtained 10 or 12 biopsy cores and only 20.8% of them obtained routine transitional zone biopsy during the initial biopsy session. Conclusion: This survey demonstrated that patient preparation and technique of ultrasound-guided prostate biopsy is not standardized among Turkish Urologists, and a guideline on prostate biopsy is needed.