Browsing by Author "Caliskan G."

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    Green Synthesis and Characterization of Titanium Nanoparticles Using Microalga, Phaeodactylum tricornutum
    (Taylor and Francis Ltd., 2022) Caliskan G.; Mutaf T.; Agba H.C.; Elibol M.
    Nanoparticles synthesized from microalgae offer a newly discovered process that is open to improvement. In this study supernatant of Phaeodactylum tricornutum (P. tricornutum) culture was used for this purpose. Firstly, the effects of some variables, namely titanium concentrations, titanium:supernatant ratio, pH, incubation time, and mixing speed on nanoparticle synthesis were investigated by using statistical design method in shaken culture as well as bioreactor. The average nanoparticle size synthesized in optimum conditions which were pH 7.5 with 300 rpm for 1 h was found as 50 nm. In the second part, nanoparticles were coated with a chitosan solution to protect their stability and increase their potential. Therefore, the antimicrobial activity showed a reasonable effect on these nanoparticles coated with chitosan. Nanoparticles produced had interestingly shown 99% antistatic properties. According to the cytotoxicity test, these nanoparticles showed a high cytotoxic effect on different cancer cell lines. The results obtained in the present study can be considered promising outcomes for possible future antimicrobial, biogenic and antistatic studies particularly in biomedical applications. © 2021 Informa UK Limited, trading as Taylor & Francis Group.
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    Green synthesis of titanium nanoparticles using a sustainable microalgal metabolite solution for potential biotechnological activities
    (John Wiley and Sons Ltd, 2023) Mutaf T.; Caliskan G.; Ozel H.; Akagac G.; Öncel S.Ş.; Elibol M.
    In this study, green synthesis of titanium nanoparticles using liquids metabolites of microalgae, Porphyridium cruentum, was performed to evaluate potential biotechnological activity. The rising rates of multidrug-resistant bacteria and the number of cancer patients are driving the search for novel antimicrobial and anticancer agents to combat this threat. In recent years, with the increasing number of studies, nanomaterials are starting to be better understood and are emerging as a solution to this problem. Especially, green synthesized nanoparticles with anticancer, antioxidant, and antimicrobial activities have potential in biomedical applications because of their eco-friendly and biocompatible nature. Scanning electron microscopy (SEM) images revealed that spherical shaped Ti-NPs' size ranged from 62 to 133 nm. This study aimed to assess the effectiveness of antibacterial activity of Ti-NPs and chitosan-coated Ti-NPs against Escherichia coli and Staphylococcus aureus using disc diffusion assay. It demonstrated the concentration-dependent cytotoxic effect of Ti-NPs of human prostate adenocarcinoma (PC-3), human alveolar adenocarcinoma (A549), and human mammary gland adenocarcinoma (MDA-MB) cancer cell lines. This present study shows promising outcomes for possible future applications of synthesized Ti-NPs as a novel antibacterial and cytotoxic agent for biomedical applications such as drug delivery, biosensor, and hyperthermia. © 2023 Curtin University and John Wiley & Sons, Ltd.