Browsing by Author "Can Ş."

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    Essential oil composition and antioxidant activity of Thymus longicaulis C. Presl subsp. longicaulis var. longicaulis
    (2010) Sarikurkcu C.; Sabih Ozer M.; Eskici M.; Tepe B.; Can Ş.; Mete E.
    This study is designed to examine the chemical composition and in vitro antioxidant activity of the hydrodistillated essential oil and various extracts obtained from Thymus longicaulis subsp. longicaulis var. longicaulis. GC and GC-MS analysis of the essential oil were resulted in determination 22 different compounds, representing 99.61% of total oil. γ-terpinene, thymol and p-cymene were determined as the major compounds of the oil (27.80, 27.65 and 19.38%, respectively). Antioxidant activities of the samples were determined by four different test systems namely β-carotene/linoleic acid, DPPH, reducing power and chelating effect. Essential oil showed the highest antioxidant activity in β-carotene/linoleic acid system among the experiments examined. In the case of other test systems, in general, methanol and water extracts exhibited the strongest activity profiles. Especially, reducing power of water extract was found superior than those of synthetic antioxidants. As well as the antioxidant activities of the extracts, they were evaluated in terms of their total phenolic and flavonoid contents. Hexane and water extracts were found to be rich-in phenolics. However, flavonoids were determined in the highest level in methanol extract. © 2010 Elsevier Ltd.
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    The frequency and associated factors of diabetic ketoacidosis at diagnosis in children with type 1 diabetes; [Tip 1 diyabetli çocuklarda tanıda diyabetik ketoasidoz sıklıǧı ve i̇liflkili faktörler]
    (Galenos Yayincilik,, 2010) Demir K.; Büyükinan M.; Dizdarer C.; Şimşek D.G.; Özen S.; Asar G.; Can Ş.; Altincik A.; Özhan B.; Ersoy B.; Böber E.; Darcan Ş.
    Introduction: In this study, it was aimed to assess the frequency and associated factors of diabetic ketoacidosis (DKA) at diagnosis in patients with newly diagnosed type 1 diabetes who were admitted to pediatric endocrinology clinics in tertiary referral hospitals in Izmir and Manisa provinces. Materials and Method: The files of the patients were evaluated retrospectively. Data regarding sex, date of birth, family history for diabetes, and health insurances of the patients were recorded and compared with respect to the form of clinical presentation. Results: It was noted that 139 patients (M/F:74/65, mean age 8.7±3.9 years) were diagnosed in 2008. At the time of diagnosis, the clinical picture of the majority of the patients were ketosis (n=58, 41.7%) or DKA (n=57, 41%). Mortality or severe morbidity developed in none of the patients. It was detected that lack of family history for type 1 diabetes and being less than 5 years of age were associated with DKA at the time of diagnosis. When logistic regression analysis was used to perform risk analysis, only being less than 5 years of age was found to be a risk factor for DKA (p=0.008, Odds Ratio 3.3, 95% confidential interval 1.4-8.1). Conclusion: These results led us consider that large-scale campaigns/studies are needed to be performed to reduce the ratio of DKA at the time of diagnosis by making the society conscious of diabetes in childhood. © The Journal of Current Pediatrics, published by Galenos Publishing.
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    Current practice in diagnosis and treatment of growth hormone deficiency in childhood: A survey from Turkey
    (Galenos Yayincilik,, 2015) Poyrazoğlu Ş.; Akçay T.; Arslanoğlu I.; Atabek M.E.; Atay Z.; Berberoğlu M.; Bereket A.; Bideci A.; Bircan I.; Böber E.; Can Ş.; Cesur Y.; Darcan Ş.; Demir K.; Dündar B.; Ersoy B.; Esen I.; Güven A.; Kara C.; Keskin M.; Kurtoğlu S.; Memioğlu N.; Özbek M.N.; Özgen T.; Sari E.; Şiklar Z.; Şimşek E.; Turan S.; Yeşilkaya E.; Yuksel B.; Darendeliler F.
    Objective: Approaches to diagnosis and treatment of growth hormone deficiency (GHD) in children vary among countries and even among centers in the same country. This survey, aiming to facilitate the process of preparing the new consensus on GHD by the Turkish Pediatric Endocrinology and Diabetes Society, was designed to evaluate the current practices in diagnosis and treatment of GHD in different centers in Turkey. Methods: A questionnaire covering relevant items for diagnosis and treatment of GHD was sent out to all pediatric endocrinology centers. Results: Twenty-four centers returned the questionnaire. The most frequently used GH stimulation test was L-dopa, followed by clonidine. Eighteen centers used a GH cut-off value of 10 ng/mL for the diagnosis of GHD; this value was 7 ng/mL in 4 centers and 5 ng/mL in 2 centers. The most frequently used assay was immunochemiluminescence for determination of GH, insulin-like growth factor-1 and insulin-like growth factor binding protein-3 concentrations. Sex steroid priming in both sexes was used by 19 centers. The most frequently used starting dose of recombinant human GH (rhGH) in prepubertal children was 0.025-0.030 mg/kg/day and 0.030-0.035 mg/kg/day in pubertal children. Growth velocity was used in the evaluation for growth response to rhGH therapy in all centers. Anthropometric measurements of patients every 3-6 months, fasting blood glucose, bone age and thyroid panel evaluation were used by all centers at follow-up. Main indications for cessation of therapy were decreased height velocity and advanced bone age. Fourteen centers used combined treatment (rhGH and gonadotropin-releasing analogues) to increase final height. Conclusion: Although conformity was found among centers in Turkey in current practice, it is very important to update guideline statements and to modify, if needed, the approach to GHD over time in accordance with new evidence-based clinical studies. © Journal of Clinical Research in Pediatric Endocrinology.
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    In vitro anti-leishmanial activity of Sarcopoterium spinosum against Leishmania tropica; [Sarcopoterium spinosum’un Leishmania tropica’ya karşı in vitro anti-leishmanial etkisi]
    (Chartered Inst. of Building Services Engineers, 2018) Can H.; Kayalar H.; Bozkurt B.; Can Ş.; Döşkaya M.; Töz S.
    Complex clinical symptoms such as ulcerative skin lesions, destructive mucosal inflammation, and disseminated visceral infection can reveal in leishmaniasis. The conventional drugs are toxic and expensive. In addition, patients receive a long treatment with these drugs which have adverse effects and unfortunately there are some limitations during the treatment. The aim of this study is to investigate the in vitro anti-leishmanial activities of four different extracts of Sarcopoterium spinosum against Leishmania tropica. Initially, different concentrations of ethanol, methanol, n-hexane, and water extracts of S. spinosum were incubated with L. tropica promastigotes. After 72 hours of incubation, the growth of L. tropica promastigotes was significantly inhibited and the percentage of inhibition ranged between 42.8 and 100 %. Among these extracts, the most efficient growth inhibition (100 %) was obtained with methanol extract (at a dose of 50 µg/ml). In conclusion, S. spinosum may be a potential source for the development of novel therapeutic agents to treat L. tropica infection. © 2018, Chartered Inst. of Building Services Engineers. All rights reserved.
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    Prevalence of toxoplasmosis and genetic characterization of Toxoplasma gondii strains isolated in wild birds of prey and their relation with previously isolated strains from Turkey
    (Public Library of Science, 2018) Karakavuk M.; Aldemir D.; Mercier A.; Şahar E.A.; Can H.; Murat J.-B.; Döndüren Ö.; Can Ş.; Özdemir H.G.; Döşkaya A.D.; Pektaş B.; Dardé M.-L.; Gürüz A.Y.; Döşkaya M.
    Toxoplasma gondii is a protozoon parasite that causes congenital toxoplasmosis, as well as other serious clinical presentations, in immune compromised humans. Analyses of the prevalence and genotyping of strains from the definitive host and intermediate hosts will help to understanding the circulation of the different strains and elucidating the role of the genotype (s) in human toxoplasmosis. Turkey has a specific geographic location bridging Africa, Europe, and Asia. We hypothesized that T. gondii strains may have been transferred to Turkey from these continents via migratory birds or vice versa. The present study aimed to assess the prevalence of toxoplasmosis in wild birds of prey of İzmir and Manisa provinces as well as genetically characterize T. gondii strains from these wild birds to show the relation between bird strains and neighboring stray cats as well as human strains previously isolated in Turkey. Tissues obtained from 48 wild birds were investigated for the presence of T. gondii DNA and then bioassayed in mouse. Isolated strains were genotyped using 15 microsatellite markers. The prevalence of T. gondii DNA was found to be 89.6% (n: 43/48) in wild birds. Out of 43 positive samples, a total of 14 strains were genotyped by 15 microsatellite markers. Among them, eight were type II, three were type III and three were mixture of genotypes (two type II/II and one was II/III). These are the first data that showed the presence of T. gondii and types II and III genotypes in wild birds of Turkey. Moreover, Africa 1 was not detected. In addition, cluster analysis showed that T. gondii strains within type II and III lineage have close relation with strains previously isolated from stray cats in İzmir. Further studies are required to isolate more strains from human cases, other intermediate hosts, and water sources to reveal this relation. © 2018 Karakavuk et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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    Development of a “Rapid-Crypto Colorimetric LAMP Test” to Detect Cryptosporidiosis in Feces of Newborns Calves
    (Springer Science and Business Media Deutschland GmbH, 2024) Karakavuk M.; Can H.; Can Ş.; Karakavuk T.; Döşkaya M.; Değirmenci Döşkaya A.
    Background: Cryptosporidiosis is a disease that causes major intestinal damage in humans and animals. The causative agents of the disease are Cryptosporidium species. In newborn calves, diarrhea can lead to death, resulting in significant economic losses for the farms. Therefore, accurate, rapid, and cost-effective diagnosis of the disease is very important. Material and methods: In this study, a novel colorimetric loop-mediated isothermal amplification (LAMP) test named “Rapid-Crypto Colorimetric LAMP test” targeting Cryptosporidium spp. 18S rRNA gene was developed to detect cryptosporidiosis in the feces of newborn calves. The analytical sensitivity of the test was determined by plasmid controls. Clinical sensitivity was determined using the feces of 127 calves collected from farms in İzmir and Manisa provinces. All of the samples were also investigated with Real-Time PCR targeting the Cryptosporidium spp. COWP gene. Cross-reactivity was tested using the DNA of other parasites and bacteria. Results: According to the results, the analytical sensitivity of the “Rapid-Crypto Colorimetric LAMP test” was found as 1 copy plasmid/reaction. When the results were compared with the Real-Time PCR test, the sensitivity of the “Rapid-Crypto Colorimetric LAMP test” was 100% and the specificity was 97.4%. The test did not cross-react with other parasites and bacteria. Conclusion: The “Rapid-Crypto Colorimetric LAMP test” developed in this study provides an advantage in the diagnosis of Cryptosporidium spp. in calf stool samples since it can be applied in basic laboratories or in the field, does not require experienced personnel, and has high sensitivity. Moreover, diagnosis can be made with the naked eye without using any device. © The Author(s) 2024.