Browsing by Author "Colak A."

Now showing 1 - 3 of 3
  • No Thumbnail Available
    Item
    The effects of meloxicam on neural tube development in the early stage of chick embryos; [Meloksikamın erken dönem civciv embriyosunda nöral tüp geliflimine etkileri]
    (Turkish Neurosurgical Society, 2010) Cetinkal A.; Colak A.; Topuz K.; Demircan M.N.; Simsek H.; Berber U.; Umur A.S.; Selcuki M.; Vatansever H.S.
    AIM: The aim of this study is to demonstrate the effect of meloxicam in early stage chick embryos on neural tube development. MATERIAL and METHODS: One hundred specific pathogen-free (SPF) chicken eggs were used to investigate the neurulation. SPF eggs were invastigated in four groups (n:25). All of the groups were incubated at 37.2 ± 0.1°C and 60 ± 5 % relative humidity for 30 hours, and an embryological development in the ninth stage as classified by Hamburger and Hamilton was obtained. In the end of the 30th hour, group A(control group) was administered 0.1 ml of saline (0.9% NaCl) in ovo and the other groups were administered meloxicam in increasing doses. At the end of 72 hours, all of the embryos were extracted from eggs and they underwent pathological examination with hematoxylin eosine and immunohistopathological examinations with CD138 and tubulin beta II. RESULTS: While the groups Aand B showed no neural tube defects, totally eight defective embryos were detected in the groups C and D (three in group C and five in group D. CONCLUSION: Our results suggested that meloxicam, a nonselective COX inhibitor, caused neural tube closure defects when injected at supratherapeutic doses. However, further studies with larger numbers of subjects are needed for its use in lower doses.
  • No Thumbnail Available
    Item
    Determination of a predictive cutoff value of NT-proBNP testing for long-term survival in ED patients with acute heart failure
    (2013) Velibey Y.; Golcuk Y.; Golcuk B.; Oray D.; Atilla O.D.; Colak A.; Kurtulmus Y.; Erbay A.R.; Yilmaz A.; Eren M.
    Objective The main objective of this study was to determine a predictive cutoff value for plasma N-terminal pro-B-type natriuretic peptide (NT-proBNP) that could successfully predict the long-term (4-year) survival of patients with acute heart failure (HF) at the time of admission to the emergency department (ED). To our best knowledge, our study is the first research done to identify a predictive cutoff value for admission NT-proBNP to the prescriptive 4-year survival of patients admitted to ED with acute HF diagnosis. Methods NT-proBNP levels were measured in plasma obtained from 99 patients with dyspnea and left ventricular dysfunction upon admission to the ED. The end point was survival from the time of inclusion through 4 years. Results The mean age of the patients in this study was 71.1 ± 10.3 years; 50 of these patients were female. During the 4-year follow-up period, 76 patients died; survivors were significantly younger than non-survivors (64.26 ± 11.42 years vs72.83 ± 11.07 years, P =.002). The optimal NT-proBNP cutoff point for predicting 4-year survival at the time of admission was 2300 pg/mL, which had 85.9% sensitivity and 39.1% specificity (95% confidence interval, area under the curve: 0.639, P =.044). Conclusion Elevated NT-proBNP levels at the time of admission are a strong and independent predictor of all-cause mortality in patients with acute HF 4 years after admission. Furthermore, the optimal cutoff level of NT-proBNP used to predict 4-year survival had high sensitivity. However, especially in the case of long-term survival, additional prospective, large, and multicenter studies are required to confirm our results. © 2013 Elsevier Inc.
  • No Thumbnail Available
    Item
    An important source of preanalytical error in medical laboratories: Centrifugation
    (De Gruyter Open Ltd, 2021) Sonmez C.; Gümüs A.; Senes M.; Aykal G.; Taneli F.; Aksungar F.; Avci E.; Coskun C.; Çinaroglu I.; Colak A.; Eker P.; Güçel F.; Hakligor A.; Inal B.B.; Orhan B.; Yilmaz C.
    Centrifugation separates particles within the specimen according to their shape, dimensions, and density and basically can be defined as a separation method. The centrifuge is an essential device in medical laboratories to prepare the serum, plasma, and urine samples for analysis. It is basically an electric device composed of the stationary (motor) and the motile (rotor) part. The centrifugation depends on two main variables: relative centrifugal force (RCF) and centrifugation time. The physical impact separating the specimen into its components in the centrifuge known as RCF is expressed as the multiples of gravitational acceleration (×g). RPM, defined as the number of rotations of the centrifuge perminute, shows the speed of the centrifuge. RCF value can be calculated by using RPM, and the centrifuge radius. Because models and sizes of centrifuges vary considerably, the use of gravity (g) forces instead of RPM is suggested. The centrifuges can be classified according to their usage, speed, technical specifications, and rotor type. An accurate and precise centrifugation process is essential to prevent errors in the preanalytical phase. The purpose of this document is to ensure the standardization of a good, precise protocol for the centrifugation process among the medical laboratories. © 2021 De Gruyter. All rights reserved.