Browsing by Author "Deliloglu-Gurhan I."
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Item The effect of osteogenic medium on the adhesion of rat bone marrow stromal cell to the hydroxyapatite(2006) Deliloglu-Gurhan I.; Tuglu I.; Vatansever H.S.; Özdal-Kurt F.; Ekren H.; Taylan M.; Sen B.H.Objective: To investigate the adhesive properties of bone marrow stromal cell (BMSC) on the hydroxyapatite (HA) particles and analyze their behavior. Methods: The study took place in the Department of the Histology and Embryology, Celal Bayar University, Manisa and in the Department of Bioengineering, Ege University, Izmir, Turkey between 2004 and 2005. We cultured BMSC from the mature rat tibia and differentiated to the osteoblasts by osteogenic medium. The BMSCs were subcultured and were taken to the HA substrate. We measured their proliferation capacity and viability with MTT assay using the spectrophotometric method. Furthermore, we identified the osteoblast-like cells by inummohistochemical staining of osteonectin and osteocalcin and we analyzed the behavior of the cells on different sized HA particles by SEM at the end of 3 days incubation. Results: Osteogenic medium caused the proliferation capacity of BMSC to speed up and the effects appeared earlier. We confirmed the osteoblastic differentiation by staining of most cells with osteoblastic markers. Subcultured cells were similarly adhesive to the HA particles and the osteogenic medium did not alter this behavior. They spread on the substrate similarly. Most of the cells demonstrated the cytoplasmic protrusion. Morphology of the cells did not change much with or without osteogenic medium. Different sizes of HA particles did not affect the adhesive properties of these cells except HA gel. The spreading and attachment ratios of the cells on HA gel were more than the others. Conclusion: We found that there was heterogeneity in BMSC on differentiation capacity to the osteoblast, which was a sign of a subpopulation. Adhesive cells showed similar morphology and behavior under the effect of osteogenic medium. The only difference was the spreading capacity on the HA gel where cell used this substrate more effectively for adhesion.Item Ion implantation inhibits cell attachment to glassy polymeric carbon(2007) Zimmerman R.; Deliloglu-Gurhan I.; Ozdal-Kurt F.; Sen B.H.; Rodrigues M.; Ila D.Implantation of MeV gold, oxygen, carbon ions into GPC alters the surface topography of GPC and enhances the already strong tendency for cells to attach to GPC. We have shown that implantation of silver ions near the surface strongly inhibits cell growth on GPC. Both enhanced adhesion of and inhibition of cell growth are desirable improvements on cardiac implants that have long been successfully fabricated from biocompatible glassy polymeric carbon (GPC). In vitro biocompatibility tests have been carried out with model cell lines to demonstrate that ion beam assisted deposition (IBAD) of silver, as well as silver ion bombardment, can favorably influence the surface of GPC for biomedical applications. © 2007 Elsevier B.V. All rights reserved.Item Neural cell attachment on metal ion implanted glass surfaces(2012) Sokullu-Urkac E.; Oztarhan A.; Deliloglu-Gurhan I.; Gulce-Iz S.; Feyzan O.-K.; Brown I.G.We have explored the application of ion implantation as a tool for the enhancement of neural cell growth on glass surfaces. Glass substrates were ion implanted with gold and with carbon using a metal vapor vacuum arc (MEVVA) ion source-based implantation system at Ege University Surface Modification Laboratory. The implantation dose was varied over the range 10 14-1017 ions/cm2 and the ion energy spanned the range 20-80 keV. B35 neural cells were seeded and incubated on the implanted substrates for 48h at 37°C. After 2-days in culture the cell attachment behavior was characterized using phase contrast microscopy. The adhesion and direct contact of neural cells on these ion implanted glass surfaces were observed. © 2011 Materials Research Society.