MCBÜ Açıkerişim Sistemi :: Browsing by Author "Deliormanli, AM"

Browsing by Author "Deliormanli, AM"

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Structural and luminescent properties of Er3+ and Tb3+-doped sol-gel-based bioactive glass powders and electrospun nanofibers
Deliormanli, AM; Rahman, B; Oguzlar, S; Ertekin, K
In this study, sol-gel-based erbium (Er3+), terbium (Tb3+) and Er3+: Tb-3 co-doped 1393 bioactive glass powders and electrospun nanofibers were prepared. Structural and morphological properties of the bioactive glasses as well as the photoluminescence characteristics were investigated in detail. The median particle size and average diameter of the prepared glass powders and fibers were in the range of similar to 1.5-3.5 mu m and 280-660 nm, respectively. The steady-state photoluminescence and decay kinetics of the samples were investigated under excitation (374 nm) where only Er3+ and Tb3+ ions close to Si nanoclusters can be excited. All the samples prepared in the study exhibited bright green emission upon excitation at 374 nm. Results showed that the dopant concentration and the sample morphology have significant influence on the photoluminescence and decay properties of the glasses. Sol-gel-derived bioactive glass particles exhibited stronger emission intensity, whereas electrospun nanofibers showed extended decay times. In vitro bioactivity experiments revealed that Er3+ and Tb3+ doping did not inhibit the conversion of the glass samples to hydroxyapatite treated in simulated body fluid for 30 days. It was concluded that Er3+ and Tb3+-containing 1393 bioactive glasses have a potential to be used in tissue engineering applications as well as bioimaging studies.
Emission based response of Er3+, Tb3+, and Er3+-Tb3+co-doped 1393 bioactive glasses along with HPTS towards CO2
Ulucan, U; Oguzlar, S; Deliormanli, AM; Ertekin, K
The HPTS serves as a versatile tool in pH and CO2 sensing studies, as well as in cell biology studies of various processes. However, the long-term photostability of HPTS as a fluorescent dye is an important consideration in its use, especially for long-term applications. In this work, sol-gel synthesized Er3+, Tb3+ and Er3+: Tb3+ co-doped 1393 bioactive glass particles were incorporated with the HPTS in the presence of ionic liquid in an ethyl cellulose matrix. The interaction of the bioactive glasses with the HPTS was investigated by steady-state luminescence and excited-state lifetime measurements. The prepared composites showed linearizable responses when exposed to different CO2 concentrations. High I 0 /I 100 values (87, 109 and 115) were obtained for the binary blends of HPTS and bioactive glasses. When the imidazolium-based IL was used together with the offered composites, we observed improved stability and longevity for the HPTS up to 315 days. The incorporation of imidazolium-based ionic liquids as additives in HPTS-based fluorescence assays together with the bioactive glasses holds promise for enhancing the photostability of the HPTS and improving the reliability and longevity of fluorescence signals in various research applications.
In vitro assessment of degradation and bioactivity of robocast bioactive glass scaffolds in simulated body fluid
Deliormanli, AM
In this study porous three-dimensional scaffolds of borate (13-93B3) bioactive glass were prepared by robocasting and in vitro degradation and bioactivity was evaluated. Grid like scaffolds with interconnected pores was assembled using robotic deposition technique which is a direct ink writing method. After binder burnout, the constructs were sintered for 1 h at 560 degrees C to produce scaffolds (porosity approximate to 60%) consisting of dense glass struts (300 +/- 20 mu m in diameter) and interconnected pores of width 580 +/- 20 mu m. Hydroxyapatite formation on borate bioactive glass scaffolds was investigated in simulated body fluid (SBF) using three different scaffold/SBF (S/S) ratios (1, 2 and 10 mg/ml) at 37 degrees C. When immersed in SBF, degradation rate of the scaffolds and conversion to a calcium phosphate material showed a strong dependence to the S/S ratio. At high solid concentration (10 mg/ml) surface of the glass scaffolds converted to the calcium rich amorphous calcium phosphate after 30 days. At lower solid concentrations (2 and 1 mg/ml) an amorphous calcium phosphate layer formation was observed followed by the conversion to hydroxyapatite. (c) 2012 Elsevier Ltd and Techna Group S.r.l. All rights reserved.
Effect of pore architecture on the mesenchymal stem cell responses to graphene/polycaprolactone scaffolds prepared by solvent casting and robocasting
Deliormanli, AM; Atmaca, H
In the study graphene-containing porous, three dimensional polycaprolactone (PCL) scaffolds were prepared by solvent casting-salt leaching and robocasting methods for tissue engineering applications. Graphene nanopowders in the form of nanoflakes were incorporated into the polymer matrix at different concentrations namely 1, 3, 5 and 10 wt%. The dichloromethane was used as the solvent and sodium chloride crystals were utilized as the water-soluble porogen for the formation of an interconnected porous network (with non-oriented pores) inside the composite scaffolds in solvent casting-salt leaching method. On the other hand, acetone was utilized as solvent and PCL solutions were prepared at 20 wt% in robocasting method to construct scaffolds (with oriented pores) having a grid-like structure. The biological response of bone marrow mesenchymal stem cells seeded on these composite constructs having different architecture were tested using MTT method, live-dead cell viability assay and Alcian blue stanining. Cytotoxicity experiments revealed that mesenchymal stem cells did not show toxic response to composite robocast scaffolds. Cells proliferate and differentiate well on the surface of the robocast scaffolds compared to solvent-cast scaffolds under the same conditions. Results showed that scaffolds prepared in the study have potential to be used in cartilage tissue engineering in the presence of electric stimulation.
Response of mouse bone marrow mesenchymal stem cells to graphene-containing grid-like bioactive glass scaffolds produced by robocasting
Deliormanli, AM; Türk, M; Atmaca, H
In the study, three-dimensional, grid-like silicate-based bioactive glass scaffolds were manufactured using a robotic deposition technique. Inks were prepared by mixing 13-93 bioactive glass particles in Pluronic (R) F-127 solution. After deposition, scaffolds were dried at room temperature and sintered at 690 degrees C for 1 h. The surface of the sintered scaffolds was coated with graphene nanopowder (1, 3, 5, 10 wt%) containing poly(epsilon-caprolactone) solution. The in vitro mineralization ability of the prepared composite scaffolds was investigated in simulated body fluid. The surface of the simulated body fluid-treated scaffolds was analyzed using scanning electron microscopy to investigate the hydroxyapatite formation. Mechanical properties were tested under compression. Results revealed that graphene coating has no detrimental effect on the hydroxyapatite forming ability of the prepared glass scaffolds. On the other hand, it decreased the compression strength of the scaffolds at high graphene concentrations. The prepared grid-like bioactive glass-based composite scaffolds did not show toxic response to bone marrow mesenchymal stem cells. It was shown that stem cells seeded onto the scaffolds attached and proliferated well on the surface. Cells seeded on the scaffolds surface also demonstrated osteogenic differentiation under in vitro conditions in the absence of transforming growth factors.
Hexagonal Boron Nitride/PCL/PLG Coatings on Borate Bioactive Glass Scaffolds for Bone Regeneration
Ensoylu, M; Deliormanli, AM; Atmaca, H
In this study, hexagonal boron nitride (hBN) nanoparticle- containing (0.1-2 wt%) polycaprolactone (PCL) and polylactic-co-glycolic acid (PLG)-coated 13-93B3 borate-based porous bioactive glass composite scaffolds were prepared by polymer foam replication method and their ability to use in bone tissue engineering applications was assessed. Morphological, mechanical properties, cytotoxicity and the drug release behavior of the prepared composite scaffolds were investigated. In vitro bioactivity was tested in simulated body fluid and results were analyzed using FTIR spectrometer and SEM. Results showed that both polymer coating and the existence of hBN nanoparticles in the polymeric matrix improved the compressive strength of the fabricated composite scaffolds. Incorporation of the hBN nanoparticles enhanced the in vitro hydroxyapatite forming ability of the glass composites. Results also revealed that prepared bioactive glass based composite scaffolds showed no toxicity to MC3T3-E1 cells under in vitro conditions up to 72 h and hBN-containing glass scaffolds showed higher gentamicin sulfate release rates compared to the bare polymer coated scaffolds. Manufactured bioactive glass scaffolds containing hBN nanoparticles are found to be promising for bone repair and regeneration.
Preparation of trivalent rare-earth element-substituted bioactive glass robocast scaffolds for osteosarcoma treatment: structural, morphological, mechanical, drug delivery, and biological properties
Deliormanli, AM; Ensoylu, M; Atmaca, H
In this study, trivalent rare-earth ion (Eu3+, Gd3+, and Yb3+)-substituted silicate-based bioactive glass scaffolds were prepared by robocasting method using sol-gel-derived bioactive glass powders for tissue engineering applications and cancer therapy. The structural, morphological, and mechanical properties of the prepared scaffolds as well as their in vitro bioactivity in simulated body fluid (SBF) were investigated in detail. In addition, an anticancer drug (5-FU) adsorption and release behavior of the scaffolds was studied as a function of time. In vitro, cytotoxicity and alkaline phosphatase activity were investigated using human skin fibroblast BJ and osteosarcoma SaOS-2 cells. Results showed that using lanthanide ion-containing (0.5, 1, 3, and 5 wt%) sol-gel-derived bioactive glass powders it was possible to successfully fabricate periodic, mesh-like patterned robocast glass scaffolds. All of the scaffolds prepared in the study sintered at 675 degrees C showed an amorphous structure. The compressive strength of scaffolds was in the range of 8.8 MPa to 13.6 MPa and the highest strength values were obtained in the Yb3+-containing scaffolds. Hydroxyapatite formation was obtained for the scaffolds immersed in SBF for 28 days. The fluorouracil adsorption amount was calculated to be similar to 25% for all types of scaffolds and the cumulative drug release was in the range of 20-25% depending on the dopant concentration. Results of the in vitro cell culture experiments revealed that all of the scaffolds fabricated in the study were not cytotoxic to fibroblast and osteosarcoma cells for up to 7 days under in vitro conditions. An increase was obtained for the ALP activities for both types of cells as the incubation time was increased.
Evaluation of In Vitro Bioactivity, Cytotoxicity, and Drug Release Behavior of Er2O3 and Tb2O3-Containing Bioactive Glass Particles and Nanofibers
Rahman, B; Deliormanli, AM; Atmaca, H
Silicate-based bioactive glasses doped with Er3+ and Tb3+ ions (1, 3, and 5 wt%) were synthesized in the form of powders and nanofibers using sol-gel and electrospinning methods, respectively. In vitro bioactivity of the prepared powders and fibers was analyzed in simulated body fluid (SBF) for various periods, and the biological response of the osteoblastic MC3T3-E1 cells to the bioactive glass samples was studied using MTT assay and microscopic observations. The amoxicillin release behavior of the prepared glasses was examined in phosphate-buffered saline as a function of time. The results revealed that the incorporation of Er3+ and Tb3+ improved the hydroxyapatite forming ability of the prepared bioactive glass samples for up to 30 d of immersion in SBF. In vitro cytotoxicity experiments showed that Tb3+-containing glass samples were biocompatible at all concentrations; however, in the case of Er3+-containing glass particle-based samples, a decrease in cell viability was observed starting from 3 wt% Er3+. SEM observations revealed cellular adhesion and spreading on the bioactive glass scaffolds. Drug delivery experiments demonstrated that after 24 h, similar to 35 to 38% of the drug was released into the medium for both bioactive glass powder and nanofiber-based samples. Bioactive glasses synthesized in the study have the potential to be used in bone tissue engineering applications.
Effect on Improving CO2 Sensor Properties: Combination of HPTS and γ-Fe2O3@ZnO Bioactive Glass
Oguzlar, S; Ongun, MZ; Deliormanli, AM
8-Hydroxypyrene-1,3,6-trisulfonic acid (HPTS) dye, a fluorescent dye often used as a pH indicator, is embedded within the bioactive glass matrix and undergoes changes in its fluorescent properties when exposed to carbon dioxide (CO2). The aim of the current study is to investigate the use of bioactive glass (BG) particles containing gamma-Fe2O3@ZnO to enhance the CO2 sensitivity of HPTS. X-ray diffraction, Fourier transform infrared, scanning electron microscopy, and photoluminescence spectroscopies were used to characterize the sol-gel synthesized powders. The sensing slides were prepared in the form of a thin film by immobilizing the fluorescent dye and gamma-Fe2O3@ZnO-based additives into the poly(methyl methacrylate) matrix. The addition of gamma-Fe2O3@ZnO nanoparticles with bioactive glass additives to the HPTS improves the performance characteristics of the sensor, including the linear response range, relative signal variation, and sensitivity. Meanwhile, the CO2 sensitivities were measured as 10.22, 7.73, 16.56, 17.82, 19.58, and 42.40 for the undoped form and M, M@ZnO, 5M@ZnO-BG, 10M@ZnO-BG, and 20M@ZnO-BG NP-doped forms of the HPTS-based thin films, respectively. The response and recovery times of the HPTS-based sensing slide along with 20M@ZnO-BG NPs have been measured as 44 and 276 s, respectively. The gamma-Fe2O3/ZnO-containing BG particle-doped HPTS composites can be used as a promising sensor agent in the detection of CO2 gas in various fields such as environmental monitoring, medical diagnostics, and industrial processes.
Tungsten disulfide nanoparticle-containing PCL and PLGA-coated bioactive glass composite scaffolds for bone tissue engineering applications
Ensoylu, M; Deliormanli, AM; Atmaca, H
In the study, tungsten disulfide (WS2) nanoparticle-containing polymer-coated bioactive glass composite scaffolds were prepared for bone tissue engineering applications. Poly-epsilon-caprolactone (PCL) and poly(D,L-lactide-co-glycolide) (PLGA) were applied on the surface of the bioactive glass scaffolds fabricated by the polymer foam replication method. Results revealed that the presence of WS2 nanoparticles (0.1 to 2 wt%) embedded in polymer matrix improved the compression strength of the prepared scaffolds and their in vitro bioactivity in simulated body fluid. Composite scaffolds did not demonstrate a cytotoxic effect on pre-osteoblastic MC3T3-E1 cells after incubation for 72 h. SEM analysis showed that cells attached to the surface of the scaffolds and spread through the interconnected porous network. Gentamicin-loaded scaffolds demonstrated a controlled drug release behavior depending on the type of polymer applied on the coating layer. The presence of WS2 nanoparticles enhanced the drug release behavior of the scaffolds. It was concluded that bioactive glass-based composites fabricated in the study have the potential to be used for bone tissue engineering purposes.
Biological Response of Osteoblastic and Chondrogenic Cells to Graphene-Containing PCL/Bioactive Glass Bilayered Scaffolds for Osteochondral Tissue Engineering Applications
Deliormanli, AM; Atmaca, H
Graphene-containing 13-93 bioactive glass and poly(epsilon-caprolactone)-based bilayer, electrically conductive scaffolds were prepared for osteochondral tissue repair. Biological response of osteoblastic MC3T3-E1 and chondrogenic ATDC5 cells to the composite scaffolds was assessed under mono-culture and co-culture conditions. Cytotoxicity was investigated using MTT assay, cartilage matrix production was evaluated by Alcian blue staining, and mineralization of both types of cells in the different culture systems was observed by Alizarin red S staining. Results showed that osteoblastic and chondrogenic cells utilized in the study did not show toxic response to the prepared scaffolds under mono-culture conditions and higher cell viability rates were obtained in co-culture conditions. Larger mineralized areas were determined under co-culture conditions and calcium deposition amount significantly increased compared with that in control group samples after 21days. Additionally, the amount of glycosaminoglycans synthesized in co-culture was higher compared to mono-culture conditions. Electric stimulation applied under mono-culture conditions suppressed the viability of MC3T3-E1 cells whereas it enhanced the viability rates of ATDC5 cells. The study suggests that the designed bilayered osteochondral constructs have the potential for osteochondral defect repair.
Synthesis and characterization of cerium- and gallium-containing borate bioactive glass scaffolds for bone tissue engineering
Deliormanli, AM
Bioactive glasses are widely used in biomedical applications due to their ability to bond to bone and even to soft tissues. In this study, borate based (13-93B3) bioactive glass powders containing up to 5 wt% Ce2O3 and Ga2O3 were prepared by the melt quench technique. Cerium (Ce+3) and gallium (Ga+3) were chosen because of their low toxicity associated with bacteriostatic properties. Bioactive glass scaffolds were fabricated using the polymer foam replication method. In vitro degradation and bioactivity of the scaffolds were evaluated in SBF under static conditions. Results revealed that the cerium-and gallium-containing borate glasses have much lower degradation rates compared to the bare borate glass 13-93B3. In spite of the increased chemical durability, substituted glasses exhibited a good in vitro bioactive response except when the Ce2O3 content was 5 wt%. Taking into account the high in vitro hydroxyapatite forming ability, borate glass scaffolds containing Ce+3 and Ga+3 therapeutic ions are promising candidates for bone tissue engineering applications.
Bone Healing in Rat Segmental Femur Defects with Graphene-PCL-Coated Borate-Based Bioactive Glass Scaffolds
Basal, O; Ozmen, O; Deliormanli, AM
Bone is a continually regenerating tissue with the ability to heal after fractures, though healing significant damage requires intensive surgical treatment. In this study, borate-based 13-93B3 bioactive glass scaffolds were prepared though polymer foam replication and coated with a graphene-containing poly (epsilon-caprolactone) (PCL) layer to support bone repair and regeneration. The effects of graphene concentration (1, 3, 5, 10 wt%) on the healing of rat segmental femur defects were investigated in vivo using male Sprague-Dawley rats. Radiographic imaging, histopathological and immuno-histochemical (bone morphogenetic protein (BMP-2), smooth muscle actin (SMA), and alkaline phosphatase (ALP) examinations were performed 4 and 8 weeks after implantation. Results showed that after 8 weeks, both cartilage and bone formation were observed in all animal groups. Bone growth was significant starting from the 1 wt% graphene-coated bioactive glass-implanted group, and the highest amount of bone formation was seen in the group containing 10 wt% graphene (p < 0.001). Additionally, the presence of graphene nanoplatelets enhanced BMP-2, SMA and ALP levels compared to bare bioactive glass scaffolds. It was concluded that pristine graphene-coated bioactive glass scaffolds improve bone formation in rat femur defects.
Direct-write assembly of silicate and borate bioactive glass scaffolds for bone repair
Deliormanli, AM; Rahaman, MN
Silicate (13-93) and borate (13-93B3) bioactive glass scaffolds were created by robotic deposition (robocasting) of organic solvent-based suspensions and evaluated in vitro for potential application in bone repair. Suspensions (inks) were developed, characterized, and deposited layer-by-layer to form three-dimensional scaffolds with a grid-like microstructure (porosity approximate to 50%; pore width 420 +/- 30 mu m). The mechanical response of the scaffolds was tested in compression, and the conversion of the glass to hydroxyapatite (HA)-like material in a simulated body fluid (SBF) was evaluated. As fabricated, the 13-93 scaffolds had a compressive strength 142 +/- 20 MPa, comparable to the strength of human cortical bone, while the strength of the 13-93B3 scaffolds (65 +/- 11 MPa), was far higher than that for trabecular bone. When immersed in SBF, the borate 13-93B3 scaffolds converted faster than the silicate 13-93 scaffolds to an HA-like material, but they also showed a sharper decrease in strength with immersion time. Based on their high compressive strength and bioactivity, the scaffolds fabricated in this work by robocasting could have potential application in the repair of load-bearing bone. (C) 2012 Elsevier Ltd. All rights reserved.
Size-dependent degradation and bioactivity of borate bioactive glass
Deliormanli, AM
Borate bioactive glass has been shown to convert faster and more completely to hydroxyapatite and enhances new bone formation in vivo when compared to silicate bioactive glass. In this work, bioactive borate glass scaffolds, with a grid-like microstructure having different filament diameters (130 +/- 101 mu m to 300 +/- 20 mu m), were prepared by a robotic deposition technique. hi vitro degradation and hydroxyapatite formation on borate bioactive glass scaffolds were investigated in a simulated body fluid (SBF) at 37 degrees C under static conditions. Mineralization of borate and silicate bioactive glass powders was also tested under the same conditions. When immersed in SBF, degradation rate of the scaffolds and conversion to a hydroxyapatite-like material showed dependence on filament diameter. Similarly, conversion of bioactive glass particles to calcium phosphate phase strongly depends on the particle size and the sample/SBF ratio of the system. Large particles and scaffolds composed of thick struts formed less apatite and degraded less completely compared with smaller particles and thinner struts. Results showed that it is possible to tailor the degradation rate and bioactivity by changing the filament diameter of the borate bioactive glass scaffolds produced by robocasting. (C) 2013 Elsevier Ltd and Techna Group S.r.l. All rights reserved.
Synthesis and in vitro characterization of superparamagnetic γ-Fe2O3-containing 13-93 bioactive glasses for bone cancer therapy
Saatci, M; Deliormanli, AM; Atmaca, H
Osteosarcoma is one of the most common types of bone cancer, which generally starts in the long bones. In this study, superparamagnetic maghemite-containing (2, 5, 10, 20 wt%) bioactive glass powders were prepared for the treatment of osteosarcoma. For this purpose, maghemite nanoparticles were synthesized using the co -precipitation technique, and maghemite-containing bioactive glass-ceramic composites were fabricated through the sol-gel process. The structural, morphological, thermal, and magnetic properties and the in vitro bioactivity of the prepared bioactive glasses were investigated. In vitro cytotoxicity was examined using SaOS-2 and MC3T3-E1 cells. The fluorouracil (5-FU) release behavior of the studied bioactive glass powders was also monitored in phosphate-buffered saline as a function of time. Results revealed that synthesized maghemite nanoparticles as well as the maghemite-containing bioactive glass-ceramic composites have superparamagnetic properties. They have high bioactivity, with up to 5 wt% maghemite content. Prepared bioactive glass com-posites have no cytotoxicity against osteosarcoma and pre-osteoblast cells at low concentrations. Drug-loaded bioactive glass powder showed sustained release behavior. Overall results indicated that prepared glass com-posites have a high potential to be used in magnetic hyperthermia and anticancer drug release applications for the treatment of bone cancer.
Preparation, Characterization, and Drug Delivery of Hexagonal Boron Nitride-Borate Bioactive Glass Biomimetic Scaffolds for Bone Tissue Engineering
Ensoylu, M; Deliormanli, AM; Atmaca, H
In this study, biomimetic borate-based bioactive glass scaffolds containing hexagonal boron nitride hBN nanoparticles (0.1, 0.2, 0.5, 1, and 2% by weight) were manufactured with the polymer foam replication technique to be used in hard tissue engineering and drug delivery applications. To create three-dimensional cylindrical-shaped scaffolds, polyurethane foams were used as templates and covered using a suspension of glass and hBN powder mixture. Then, a heat treatment was applied at 570 degrees C in an air atmosphere to remove the polymer foam from the structure and to sinter the glass structures. The structural, morphological, and mechanical properties of the fabricated composites were examined in detail. The in vitro bioactivity of the prepared composites was tested in simulated body fluid, and the release behavior of gentamicin sulfate and 5-fluorouracil from glass scaffolds were analyzed separately as a function of time. The cytotoxicity was investigated using osteoblastic MC3T3-E1 cells. The findings indicated that the hBN nanoparticles, up to a certain concentration in the glass matrix, improved the mechanical strength of the glass scaffolds, which mimic the cancellous bone. Additionally, the inclusion of hBN nanoparticles enhanced the in vitro hydroxyapatite-forming ability of bioactive glass composites. The presence of hBN nanoparticles accelerated the drug release rates of the system. It was concluded that bioactive glass/hBN composite scaffolds mimicking native bone tissue could be used for bone tissue repair and regeneration applications.
Photoluminescence and decay characteristics of cerium, gallium and vanadium- containing borate-based bioactive glass powders for bioimaging applications
Deliormanli, AM; Oguzlar, S; Ertekin, K
Biomaterials having photoluminescent properties play a crucial role in real-time bioimaging after in vivo implantation. In this study, photoluminescence properties and decay characteristics of the borate-based 13-93B3 glasses containing different concentrations of cerium, gallium, and vanadium oxides were investigated for biomedical applications. The borate-based bioactive glass powders were prepared using melt-quench technique and size reduction was performed through planetary ball milling. Bioactivity of the prepared powders was investigated in simulated body fluid at 37 degrees C under static conditions. The photoluminescent properties and decay kinetics of the as-prepared and the SBF-treated bioactive glass powders were analyzed by steady-state and time resolved photoluminescence measurements. Results revealed that the cerium activated glasses exhibited an intense luminescence centered at 538 nm. Broad-band emission of the gallium and vanadium doped samples was centered at 440 and 572 nm, respectively. All of the SBF-treated glasses exhibited enhanced lifetimes and biexponential decays both in nanosecond and microsecond regime measurements. It was concluded that depending on the dopant concentration, bioactive glass particles prepared in the study showed remarkable photoluminescence and have potential to be used in bioimaging applications.
In vitro assessment of degradation and mineralisation of V2O5 substituted borate bioactive glass scaffolds
Deliormanli, AM
Bioactive glasses are promising scaffold materials for bone regeneration because of their unique ability to convert to hydroxyapatite (HA) in vivo, and their ability to bond with bone and soft tissues. In this study, borate based (13-93B3) bioactive glass powders containing up to 3 wt-%V2O5 were prepared by the melt quench technique. Vanadium was chosen because of its therapeutic action. It is known to promote initial wound healing and this may be especially beneficial to applications involving diabetic patients and soft tissue healing. Bioactive glass scaffolds were fabricated using the polymer foam replication method. In vitro degradation and bioactivity of the scaffolds were evaluated in simulated body fluid under static conditions. Results revealed that the vanadium-containing borate glasses have higher degradation rates compared to the bare borate glass 13-93B3. Due to low chemical durability, substituted glasses exhibited a good in vitro bioactive response. Taking into account the high in vitro HA forming ability, borate glass scaffolds containing V+5 therapeutic ions are promising candidates for bone tissue engineering applications.
In vitro cytotoxicity of magnetic-fluorescent bioactive glasses on SaOS-2, MC3T3-E1, BJ fibroblast cells, their hemolytic activity, and sorafenib release behavior
Deliormanli, AM; Rahman, B; Atmaca, H
In the study, the fabrication of superparamagnetic-fluorescent bioactive glasses in the form of the particle, nanofiber, and 3D scaffolds was performed by including maghemite (gamma-Fe2O3) nanoparticles and photoluminescent rare earth element ions (Eu3+, Gd3+, and Yb3+) using sol -gel, electrospinning, and robocasting techniques, respectively. The in vitro cytotoxicity of the magnetic-fluorescent bioactive glasses on osteosarcoma SaOS-2, pre-osteoblast MC3T3-E1, and BJ fibroblast cells, as well as their hemolytic activity and sorafenib tosylate loading and release behavior, were investigated. The cytotoxicity of the bioactive glass samples was tested using the MTT assay. Additionally, the alkaline phosphatase activity of the studied glasses was examined as a function of time. The mineralization behavior of the pre-osteoblast cell-seeded glass samples was analyzed using Alizarin red S staining. Results revealed that the in vitro cytotoxicity of the studied bioactive glasses in the form of particles and nanofibers depended on the sample concentration, whereas in the case of the 3D scaffolds, no cytotoxic response was observed on the osteosarcoma, pre-osteoblast, and fibroblast cells. Similarly, particle and nanofiber-based glass samples induced dose -dependent hemolysis on red blood cells. Drug loading rates were much lower for the 3D scaffolds compared to the particle and nanofiber-based samples. Drug release rates ranged from 25 % to 90 %, depending on the bioactive glass morphology and the pH of the release medium. It was concluded that the studied bioactive glasses have the potential to be used in tissue engineering applications and cancer therapy.

Browsing by Author "Deliormanli, AM"

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