Browsing by Author "Demirel M."
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Item Determination of fermentation and digestibility characteristics of corn, sunflower and combination of corn and sunflower silages(2008) Demirel M.; Bolat D.; Çelik S.; Bakici Y.; Eratak S.This study was conducted to determine in vivo digestibilities and fermentation property of various silages made of green herbage of Sunflower (SF), Corn (C) and corn-sunflower mixtures at different rates [75% corn+25% sunflower (75C25SF), 50% corn+50% sunflower (50C50SF), 25% corn+75 sunflower (25C75SF)] ensiled in 120 1 plastic barrels for 90 days. PH values of C and mixture silages were lower than that of SF silage (p<0.05). Concentrations of lactic, acetic, propionic and butyric acids were higher in SF silage than C silage (p<0.05). Lactic and propionic acid concentrations increased in mixture silages as the sunflower ratio increased, whereas decreases in butyric acid concentration were observed as corn ratio incresed in the mixture (p<0.05). While digestibility of DM, OM, ADF and NDF were high in C silage, digestibility of CP and EE were high in SF silage. The digestibility of DM, OM, ADF and NDF in mixture silages increased with the increases in corn ratio in mixture and digestibility of CP and EE of increased as sunflower ratio increased in mixture. It can be concluded that high quality silages could be obtained from green herbage of corn or sunflower alone, however their nutritive values could be improved if they are mixed at 50% ratio. © Medwell Journals, 2008.Item Investigation of the prevalence of amoebiasis in Izmir province and determination of Entamoeba spp. using PCR and enzyme immunoassay(2008) Kurt O.; Demirel M.; Ostan I.; Sevil N.R.; Mandiracioglu A.; Tanyuksel M.; Ak M.; Dagci H.Amoebiasis is a common and life-threatening disease. The discrimination of the pathogenic Entamoeba histolytica from the non-pathogenic Entamoeba dispar could be done by advanced methods such as enzyme immunoassay (EIA) and PCR. The aim of this study was to investigate the prevalence of amoebiasis in Izmir province, and differentiate the Entamoeba species by PCR and EIA. Stool samples of 2,047 individuals were examined by direct microscopy, formalin ethyl acetate concentration, trichrome staining and culture, and those found to be positive for E. histolytica/dispar by any of these methods were further analyzed by PCR and EIA for species identification. Fifty-nine of 2,047 (2.9%) stool samples were found to be positive for E. histolytica/dispar with microscopy and/or culture. Among these positive samples, E. histolytica was detected in 14 (23.7%) and 5 (8.5%) samples with PCR and antigen-specific ELISA (EIA), respectively. E. dispar was diagnosed in 31 (52.5%) and 52 (88.1%) of 59 samples with species-specific PCR and EIA, respectively. Risk factors related to infection with Entamoeba spp. and other intestinal parasites included living in shanty houses (p<0.01), a history of recent immigration to Izmir (p<0.01), having no social security (p<0.05) and living with a crowded family (p<0.01). The results demonstrated the significance of amoebiasis as a public health problem among people with low socio-economic status in Izmir province.Item A possible link between subtype 2 and asymptomatic infections of Blastocystis hominis(2008) Dogruman-Al F.; Dagci H.; Yoshikawa H.; Kurt Ö.; Demirel M.Blastocystis hominis is one of the most common eukaryotic organisms in the intestinal tract of humans, while its pathogenic potential is still controversial. A total of 286 stool samples obtained from adult and pediatric patients with or without gastrointestinal symptoms in two hospitals in Manisa, Turkey, were cultured to detect B. hominis infection. Forty-one and 51 isolates were obtained from the adults and children, respectively, and these isolates were subjected to subtyping by polymerase chain reaction (PCR) with the known sequence-tagged site primers. The correlation between the genotype and the symptoms was evaluated. PCR subtyping indicated that subtype 3 was the most common genotype in both symptomatic and asymptomatic groups, and the second common genotype was subtypes 1 and 2 in symptomatic and asymptomatic groups, respectively. A significant correlation between subtype 2 and the asymptomatic groups was found among both in pediatric and adult patients (χ 2 cal=4.38, df=1, p=0.044). However, there were no significant differences between the other genotypes and the symptomatic or asymptomatic groups, as well as both the age and sex of the patients. The present study suggests that subtype 2 is a non-pathogenic genotype of B. hominis. © 2008 Springer-Verlag.Item The prevalence of intestinal parasites in the province of Izmir, Turkey(2008) Dagci H.; Kurt Ö.; Demirel M.; Östan I.; Azizi N.R.; Mandiracioglu A.; Yurdagül C.; Tanyüksel M.; Eroglu E.; Ak M.Stool samples of a total of 2,047 people in Izmir province were examined by wet mount, formalin ethyl acetate concentration, and trichrome staining methods with an aim to reveal the prevalence of intestinal parasites in Izmir together with related personal and environmental risk factors. Geographical mapping showing the density and variation of the species of intestinal parasites in Izmir was done after all maps were scaled, and the coordinates were determined with GeoMedia5.0®. The prevalence of the intestinal parasites was found to be 25.6 % in Izmir, with a variation between the districts. Blastocystis hominis was the leading parasite, and the prevalence of parasites was higher in children compared to adults; however, the difference was statistically insignificant. There was also no significant difference between the parasite prevalence and sex, marital status, education, income, frequent eating outside, and habitual raw meat eating. Significant differences were found between the parasite prevalence and crowded families, early immigrants, individuals with no social security, and people living in close contact with their livestock. The parasites were found to be less common among individuals who had been drinking bottled water and living in a house with a sewage system. The results demonstrated a correlation between the intestinal parasites and environmental conditions in our study group. We further plan to expand the study group to cover all regions of Turkey. © 2008 Springer-Verlag.Item Effect of various additives and harvesting stages on rumen degradation of sunflower silages(2009) Demirel M.; Bolat D.; Eratak S.; Çelik S.; Bakici Y.; Çelik S.; Güney M.To study effective degradability (nylon bag technique) of sunflower silage, as influenced by harvesting stage (blooming, milk or dough) or molasses (m) I urea (u) mixture, seven different silage samples for each harvest stage [sunflower (SF) and 6 mixtures (2%mSF, 4%mSF, 2%m+0.5%uSF, 2%m+1%uSF, 4%m+0.5%uSF, 4%m+1%uSF)] were incubated for 70 days in 1 litter jars. The DM and CP effective degradation of SF silage was found to be similar at blooming and milking stage and higher than that of dough stage (P<0.05). The effective degradation of ADF and NDF of SF silage at milking and dough stages was lower than that of blooming stage. The positive effects of molasses and molasses+urea additives on effective degradability were observed. It was concluded that decreased degradability of nutrient matters because of its advanced vegetational stage could be partly compensated by using different amount of molasses and molasses+urea additives. © GSP, India.Item Muscle Injuries: Strains, Contusions, and Ruptures(Springer Berlin Heidelberg, 2015) Dönmez G.; Dilicikik U.; Aydog S.T.; Evrenos M.K.; Tetik O.; Demirel M.; Doral M.N.Muscle injuries among athletic population are common and often occur during sportive activities or training. Most sports-related muscle injuries involve strains, contusions, and uncommonly ruptures. These injuries are responsible for a large proportion of time lost to competition, and particularly for all professional athletes, rapid return to training and competition is a priority. This chapter will discuss the etiology, risk factors, classification, treatment, and possible complications of common types of muscle injuries under the lights of current literature and recent trends. © Springer-Verlag Berlin Heidelberg 2012, 2015, All Rights Reserved.