Browsing by Author "Deniz, G"

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    Factors Affecting Side Effects, Seroconversion Rates and Antibody Response After Inactivated SARS-CoV-2 Vaccination in Healthcare Workers
    Senol Akar, S; Akçali, S; Özkaya, Y; Gezginci, FM; Cengiz Özyurt, B; Deniz, G; Karadag Yalçin, F; Özer, D; Dündar Erbay, P; Eser, E
    In this study, it was aimed to prospectively evaluate the efficacy, side effects and seroconversion data of inactive severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), CoronaVac (R) (Sinovac, China) vaccine in healthcare workers. A total of 1053 healthcare workers who were initially seronegative (COV2T (R) SARS-CoV-2 Total Siemens, USA) and vaccinated with inactivated SARS-CoV-2 were included in the study. Quantitative IgG antibodies (ADVIA Centaur (R) SARS-CoV-2 IgG, Siemens, USA) were investigated 28 days after the first vaccine (n=939) and the second vaccine (n=771). In addition, neutralizing antibodies were evaluated via enzyme linked immunosorbent assay (ELISA) test (ACE2-RBD Neutralization Assay, Dia-Pro, Italy) 28 days after the first vaccine. Antibody response of the vaccine was evaluated statistically by univariate (Chi-square, Fisher's exact test, Student's t test, Mann-Whitney U, one-way ANOVA and Kruskall Wallis ANOVA tests) analysis and linear regression models. The consistency between quantitative IgG test and neutralizing antibody test was also evaluated in blood samples taken 28 days after second vaccination. Statistical analysis was determined in logarithmically transformed data with statistical analysis with SPSS 23.0 and Stata, and type 1 error level was accepted as 0.05. At least one side effect was reported by 31.3% and 26.8% of the participants after the first and second vaccine, respectively. The most frequent side effect was pain at the injection site with a frequency of 20.4% vs 21.7%. The frequency of applying to a health center due to side effects was 1.0% after the first vaccine and 0.8% after the second vaccine. The percentage of those who produced sufficient quantitative IgG was found as 25.3% (95% CI=22.5-28.1) 28 days after the first vaccine and 97.9% (95% CI=96.9198.93) after the second vaccine. Neutralizing test antibody positivity was found as 97.7% 28 days after the second vaccine. In univariate analysis, the characteristics that significantly increased the quantitative IgG response against inactivated SARS-CoV-2 vaccine were young age (p<0.01), female gender (p< 0.01), being a non-smoker (p<0.001), not having a chronic disease (p=0.019), having had the flu vaccine this year (p=0.012), not being overweight or obese (p=0.020), and having a SARS-CoV2 infection prior to vaccination (p<0.001). In addition, allied health personnel showed significantly lower antibody responses than the other workers (p<0.001). Multiple linear regression models revealed that, female gender, younger age, smoking and previous COVID-19 polymerase chain reaction test positivity significantly affected the quantitative IgG response after vaccination. A 99% agreement was found between the ELISA-based neutralizing antibody test and the quantitative IgG test (Kappa p=0.783) performed on the 28th day after the second vaccination. CoronaVac (R) provides adequate antibody response in 25% of healthcare workers aged 18-64, after 28 days from a single vaccine, and 97% after 28 days from the second vaccine. Antibody response was significantly higher in younger ages, women, non-smokers, and those who had previously encountered SARS-CoV-2. Phase 3 and phase 4 results are needed to show effectiveness of this vaccine in real life.
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    Assessment of COVID-19 Contact Healthcare Workers at Manisa Celal Bayar University Hospital
    Dündar, PE; Sen Gündogan, NE; Erten, H; Deniz, G; Özkaya, Y
    Introduction: In the COVID-19 pandemic process, the health of healthcare workers with a high risk of infection is critical. Many health- care workers have become infected while serving COVID-19 patients, and they have also been and are still exposed to the disease agent at hospital, household or community-based. The aim of this study was to determine the risk status of healthcare workers in contact with COVID-19 patients working at Manisa Celal Bayar University Hospital, and to reveal COVID-19 surveillance and related results. Materials and Methods: This descriptive study was based on examining the data of healthcare workers (HW) that came in contact with the disease between 1 April and 31 December 2020 to the COVID-19 Surveillance Unit (SU), which was established by Manisa Celal Bayar University Public Health Department during the pandemic process. Results: Among the in-contact health workers; 65.7% were females, 42.5% were nurses and 26.0% were physicians. 23.8% of the contacts were high, 42.9% medium and 33.3% low risk. High risk contact percanteges were statistically higher in the emergency unit personal, laboratory workers and household/community based contacts (p= 0.000). PCR positivity rate was 10.1% in high risk con- tact and 3.6% in medium risk contact (p= 0.000). During the research period, the rate of COVID-19 positivity in healthcare workers at Manisa Celal Bayar University Hospital was determined as 21.9% (n= 374). In the study, 24.9% of COVID-19 positive healthcare workers were physicians and 29.0 % were nurses. Conclusion: 23.8% of HCW were evaluated as high risk contact and 21.9% of the group tested positive. High risk contact percanteges were statistically higher in the emergency unit personal, laboratory workers and household/community based contacts. Protecting healthcare workers from infection in the fight against pandemics and performing contact follow-ups are also vital for continuity of service.
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    Mesenchymal Stem Cells: a Potential Treatment Approach for Refractory Chronic Spontaneous Urticaria
    Özdemir, RBÖ; Özdemir, AT; Kirmaz, C; Ovali, E; Ölmez, E; Kerem, H; Evrenos, MK; Deniz, G
    The etiopathogenesis of chronic spontaneous urticaria (CSU) is not fully elucidated, and almost 30-40% of patients are resistant to treatments; therefore, there is still a need for the development of new and effective treatments. This study aimed to develop experimental cellular therapy for CSU patients resistant to current treatment options. Autologous adipose tissue mesenchymal stem cells (MSC) were administered to 10 refractory CSU patients who were then followed up for six months. The efficacy of treatment was evaluated according to the weekly urticaria activity scores (UAS7) and drug use scores (DUS7). To observe the effect of treatment on immune cells, CD4(+) T cell subsets were analyzed by flow cytometry, and the serum IFN-gamma, TNF-alpha, IL2, IL-4, IL-5, IL-6, IL-10, IL-13, IL-17a, IL-21, IL-22, TGF-beta 1, PGE2, IDO and anti-Fc epsilon RI levels were measured using the Luminex and ELISA methods. The values obtained were compared with 10 control refractory CSU patients and five healthy controls. We found that the T cell subsets and inflammatory molecules were not affected by MSC treatment during the follow-up period. In control patients, a significant decrease was detected only at the Th2 subset, TGF-beta 1, PGE2, IDO and anti-Fc epsilon RI levels on the 14th day of treatment. The UAS7 and DUS7 values of the MSC-treated patients significantly decreased during the follow-up period, but in control patients, a significant but temporary decrease was seen. According to our findings, unlike conventional treatment, MSC therapy resulted in longer and more effective recovery. Our data indicate that MSCs may be an alternative and effective approach for treatment-resistant CSU patients.