Browsing by Author "Dogan, AA"

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    Surface Area of Graphene Governs Its Neurotoxicity
    Tasdemir, S; Morçimen, ZG; Dogan, AA; Görgün, C; Sendemir, A
    Due to their uniquephysicochemical properties, graphene and itsderivatives are widely exploited for biomedical applications. It hasbeen shown that graphene may exert different degrees of toxicity inin vivo or in vitro models when administered via different routesand penetrated through physiological barriers, subsequently beingdistributed within tissues or located within cells. In this study,in vitro neurotoxicity of graphene with different surface areas (150and 750 m(2)/g) was examined on dopaminergic neuron modelcells. SH-SY5Y cells were treated with graphene possessing two differentsurface areas (150 and 750 m(2)/g) in different concentrationsbetween 400 and 3.125 mu g/mL, and the cytotoxic and genotoxiceffects were investigated. Both sizes of graphene have shown increasedcell viability in decreasing concentrations. Cell damage increasedwith higher surface area. Lactate dehydrogenase (LDH) results haveconcluded that the viability loss of the cells is not through membranedamage. Neither of the two graphene types showed damage through lipidperoxidation (MDA) oxidative stress pathway. Glutathione (GSH) valuesincreased within the first 24 and 48 h for both types of graphene.This increase suggests that graphene has an antioxidant effect onthe SH-SY5Y model neurons. Comet analysis shows that graphene doesnot show genotoxicity on either surface area. Although there are manystudies on graphene and its derivatives on their use with differentcells in the literature, there are conflicting results in these studies,and most of the literature is focused on graphene oxide. Among thesestudies, no study examining the effect of graphene surface areas onthe cell was found. Our study contributes to the literature in termsof examining the cytotoxic and genotoxic behavior of graphene withdifferent surface areas.
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    Effects of high-intensity interval training program on pituartry function in basketball players: a randomized controlled trial
    Soslu, R; Uysal, A; Devrilmez, M; Çuvalcioglu, IC; Dogan, AA; Karaburgu, S; Tas, M
    The aim of the study was to determine how the short high-interval training program affects cortisol levels in basketball players. A total of 27 male basketball players volunteered for the study and were randomly assigned to one of two groups: Control Group (CG) (n = 13, mean age; 20,56 +/- 1,45 years, mean height; 184,53 +/- 6,65 cm) and Experimental Group (EG) (n = 14, mean age; 20,71 +/- 2,12 years, mean height; 86,51 +/- 8,21 cm). The experimental group received a 7-week interval training program. Blood samples were taken at the beginning and end of the study. Cortisol, Adrenocorticotropic Hormone, Triiodothyronine, Tetraiodothyronine, Parathyroid Hormone, Thyroid Stimulating Hormone, Insulin, and Glucose levels were measured of the EG and CG. To test the differences between groups and compare the effects of pre and post-intervention, a two-way ANOVA for repeated measures was used. Consequently, the post-test levels of TSH, PTH, and ACTH in the exercise group showed a significant difference when compared to the pre-test values (p = 0.000). Moreover, the post-test levels of Glucose, Cholesterol, Triglyceride, HDL, and Mg in the exercise group showed statistical significance when compared to the pre-test values (p = 0.000). Significant differences were seen in the post-test PTH and ACTH levels of the control group when compared to the pre-test values (p = 0.000). Furthermore, the exercise group showed significant differences in post-test values for HDL and Cholesterol compared to the pre-test (p = 0.000). In addition, when comparing the post-test values and pre-test values of both the exercise group and the control group, it was found that all parameters, with the exception of Ca, exhibited substantial differences in favor of the exercise group. It may be claimed that the implementation of interval training has favorable outcomes on pituitary function parameters. Additionally, the regulation of energy consumption during exercise is favourably influenced, along with the reduction of physiological stress resulting from prolonged exercise.