Browsing by Author "Durmuskahya, C"
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Item KARYOLOGICAL STUDIES ON SOME PHLOMIS L TAXA (LAMIACEAE)Özdemir, C; Durmuskahya, C; Sepet, H; Bozdag, BIn present study the chromosome number and morphology of some species of the genus Phlomis Linnaeus (Lamiaceae) were investigated using karyological techniques. The seeds of Phlomis grandiflora H. S. Thompson, var. grandiflora and Phlomis lunariifolia Sm. were collected from natural habitats. The chromosome numbers were determined for all species as 2n = 20. The chromosome numbers and karyotype analysis of the species were here reported for the first time from Turkey.Item Ethnobotanical Survey of Medicinal Plants Used for the Treatment of Diabetes in Manisa, TurkeyDurmuskahya, C; Öztürk, MIn this study, medicinal plants which are traditionally used for treatment of diabetes mellitus in Manisa and its environs have been investigated. A detailed market survey and ethnobotanical enquiry was performed in the region. In all, 121 medicinal herb dealers were interviewed together with the patients suffering from diabetes. After questionnaire studies, plant specimens were collected from medicinal herb markets known locally as 'aktars' or 'baharatchi', a total of 27 plant taxa belonging to 15 families were identified to be used for the treatment of diabetes. These taxa mostly belonged to the families Rosaceae (5 taxa), Lamiaceae (3 taxa), Fabaceae (2 taxa) and Asteraceae (2 taxa). The most important antidiabetic plants are; Zizyphus jujube Mill. (jujube), Origanum onites L. (oregano), Ceracus mahaleb L. (mahaleb) and Trigonella foenum-graecum L. (fenugreek). Attempts are being made to investigate their active compounds responsible for the hypoglycemic activity.Item Ecology, anatomy and morphology of Orchis spitzelii in TurkeyDurmuskahya, C; Akyol, Y; Özdemir, CIn the present study reveals the morphological, anatomical and ecological characteristic of Orchis spitzelii in Turkey. Plant materials of Orchis spitzelii were collected from 17 different population, between 20102013 in Turkey. Orchis spitzelii samples were analyzed for 19 morphological, 20 anatomical and 18 soil characters and habitat properties. As a result, the minimum length of plant was 200 mm and maximum 500 mm, minimum length of underground part was 25 mm and maximum 94 mm. Number of leaves varied betvveen 3-9. According to habitat definition, Orchis spitzelii grew up to 500 m to 2100 m. Also, Pinus nigra forests and glades were most common habitat of Orchis spitzelii, found generally over calcareous soils.Item In vitro Efficacy of Quercus infectoria Oliv. and Achillea millefolium L. Extracts Against Blastocystis spp. IsolatesÖzbilgin, A; Durmuskahya, C; Kilimcioglu, AA; Kayalar, H; Kurt, Ö; Ermis, VÖ; Tabak, T; Östan, IBlastocystis is a common intestinal parasite that can inhabit the intestinal tract of humans and many animals. Despite it was firstly described almost 100 years ago; many subjects are still under debate about Blastocystis, including its life-cycle, pathogenic potential and treatment of infected individuals. Historically, local plant species have been used for therapeutic purposes by the local people of Anatolia. Here, hexane and methanol extracts of two local plants, Quercus infectoria (Fagaceae) and Achillea millefolium, which have been used against diarrhea in Anatolia, were examined for their in vitro efficacies against Blastocystis. LC50 and EC50 values of the plant extracts were determined by Brine Shrimp and Graphpad Prism 5 (R) methods, respectively. The results showed that LC50 (500 mu g/ml) and EC50 (198.8 mu g/ml) concentrations of the methanol extract of A. millefolium were lowest compared to other extracts, its anti-Blastocystis activity was found to be comparable to metronidazole and it showed no cytotoxic activity. These initial results suggest that the methanol extract of A. millefolium may be a novel option for the treatment of Blastocystis infections in humans in future, if confirmed by further, larger-scale studies.Item Elemental Analysis of Galium incanum SM subsp Centrale Ehrend by X-ray Fluorescence SpectroscopyKayalar, H; Durmuskahya, C; Hortooglu, ZSPurpose: To evaluate the content of trace elements in Galium incanum SM. subsp. centrale Ehrend. Methods: The air-dried aerial parts of the plant material were used and its water extract (2 % w/v infusion) was analysed for trace elements using x-ray fluorescence (XRF) spectrometry. Results: The aqueous extract depicted significant concentrations of macro-and micro-nutrients with heavy metal and metal oxide content of 4.07 - 6.02 and 3.19 - 4.01 % for powdered plant material and water extract, respectively. The contents of Ca (22840 ppm) and K (8204 ppm) were the highest among all the elements while Zn (45.9 ppm) and Fe (328 ppm) were also detected in significant amounts. Zn, Mn and Cu showed the highest content while those of Mg, Al, K, Ca, Fe and P lowest in the water extract. Conclusion: The presence of significant levels of Ca, K, Na, Fe, Zn, Mg, Mn and Cu in G. incanum subsp. centrale showed that this plant has notable nutrient elements. The traditional use of Gallium species as a diuretic may be attributed to its rich content of potassium.Item In vivo Antimalarial Activity of Methanol and Water Extracts of Eryngium thorifolium Boiss (Apiaceae Family) against P. berghei in Infected MiceUral, IO; Kayalar, H; Durmuskahya, C; Cavus, I; Ozbilgin, APurpose: To investigate the in vivo antimalarial effect of Eryngium thorifolium, an endemic plant in Turkey. Methods: The methanol and water extracts were prepared and phytochemical analysis conducted on the extracts. Twenty four healthy Balb/c male mice, divided into 4 groups (n = 6), were infected intravenously with Plasmodium berghei and 100 - 250 mg/kg plant extracts administered orally in a single dose per day for 5 days. The untreated group of mice received normal saline solution and chloroquine (standard drug) served as reference drug. Results: The water extract group (250 mg/kg) prolonged the survival of the mice by 6 days compared with the untreated mice while the mice that received choloroquine treatment remained alive at the end of the study of the mice. In the untreated control group, maximum parasitaemia was observed on the 10th day of infection whereas The water extract exhibited some degree of antiplasmodial activity compared to untreated control group. The mice of chloroquine treated group remained alive at the end of the study with 100 % chemosuppression (p < 0.05). In the untreated control group, maximum parasitaemia was observed on the 10th day of infection whereas in the water extract group maximum parasitaemia was attained on the 16th day of infection. The water extract of the plant showed 45.85 % chemosuppression. Phytochemical screening of the water and methanol extracts revealed the presence of flavonoids, terpenoids and tannins. Anthraquinones were positive for water extract. Conclusions: The possible active compounds responsible for the observed chemosupression may be flavonoids, terpeneoids and anthraquinones which are present in the extract. This is the first report on the in vivo antimalarial activity of E. thorifolium.Item Mineral Compositions of Some Selected Centaurea species from TurkeyKayalar, H; Durmuskahya, C; Hortooglu, ZSCentaurea calolepis Boiss., Centaurea lydia Boiss. and Centaurea polyclada DC. were investigated for major minerals and trace elements by X-ray fluorescence spectrometry. Calcium and potassium were found in maximum concentrations in Centaurea species. The results revealed that the concentrations of iron, manganese and zinc were found to be high in C. calolepis. On the other hand, copper, magnesium and phosphorous were found in higher concentrations in C. polyclada. Mineral composition of Centaurea species, will be descriptive in terms of the traditional use of this species. In addition, C. calolepis, C. lydia and C. polyclada may be utilized in nutraceuticals as potential natural sources of major minerals and trace elements.Item THE MORPHOLOGICAL AND ANATOMICAL INVESTIGATION OF TWO ENDEMIC CROCUS L. (IRIDACEAE) SPECIES OF TURKEYAkyol, Y; Durmuskahya, C; Kocabas, O; Pekönür, S; Özdemir, CThe paper reports morphological and anatomical features of Crocus asumaniae Mathew and Crocus mathewii Kerndorff et Pasche, endangered plants. Transvers sections of plant parts stem, scape and leaf were investigated. Characteristically, style is dividing into 3 slender yellow to orange branches, flowering takes place in autumn and is hysteranthous. In anatomical studies, the walls of root endodermal cells of both species show thickenings completely and hairs are found on the abaxial epidermis of leaf. Presence of sclerenchymatic tissues around the vascular bundles of the leaf is a characteristic feature.Item Determination of properties of Althaea officinalis L. (Marshmallow) fibres as a potential plant fibre in polymeric composite materialsSarikanat, M; Seki, Y; Sever, K; Durmuskahya, CThe mechanical, thermal, chemical, crystallographic and morphological properties of althaea fibres, extracted from Althaea officinalis L, was examined for the first time in this study. A. officinalis L. was obtained from Mordogan, Izmir (Turkey). After extraction process, lignin, cellulose and hemicellulose contents of althaea fibres were identified. Fourier transform infrared and X-ray photoelectron spectroscopy were utilized for surface functional groups of althaea fibres. By using X-ray diffraction analysis, CI value for althaea fibre is obtained to be 68%. The images of scanning electron microscopy were taken for observation of morphology of althaea fibres. The tensile modulus and tensile strength values of althaea fibre were obtained by single fibre tensile tests as 415.2 MPa and 65.4 GPa, respectively. Thermogravimetric analysis showed that thermal degradation of the fibre begins at approximately 220 degrees C. Besides, by pulling out the althea fibre from the embedded high density polyethylene, interfacial shear strength value was determined to be 8.16 MPa. The results suggest that the althaea fibre can be used in composite applications as a natural reinforcement material. (C) 2013 Elsevier Ltd. All rights reserved.Item Extraction and properties of Ferula communis (chakshir) fibers as novel reinforcement for composites materialsSeki, Y; Sarikanat, M; Sever, K; Durmuskahya, CThe aim of this study is to examine the use of Ferula communis fibers as potential reinforcement in polymer composites. The fibers are extracted from the F. communis plant which grows in Selcuk, Izmir in western Turkey. The chemical composition of ferula fibers in terms of cellulose, lignin, and ash contents was determined. Surface functional groups of ferula fibers were obtained by fourier transform infrared and Xray photoelectron spectroscopy. Crystallinity index and crystallite size were determined by X-ray diffraction analysis. The morphology of ferula fibers was investigated through scanning electron microscopy, the thermal behavior through thermogravimetric and differential scanning calorimetry analyses. The real density of ferula fibers was measured by means of Archimedes method with ethanol. The mechanical properties of F. communis were measured through single fiber tensile tests. The interfacial shear strength (IFSS) in a polyester matrix has been estimated from the pull-out test. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.Item STUDIES ON THE MORPHOLOGY, ANATOMY AND ECOLOGY OF OPHRYS LUTEA CAV. SUBSP MINOR (GUSS.) O. DANESCH & E. DANESCH EX GOLZ & HR REINHARD (ORCHIDACEAE) IN TURKEYDurmuskahya, C; Ozdemir, C; Bozdag, B; Öztürk, MIn this study, morphological, anatomical and ecological characteristics of Ophrys lutea subsp. minor in Turkey were investigated. The plant material was collected from 30 different populations during 2010-2013. In all 19 morphological and 20 anatomical features were investigated, and habitat characteristics recorded. The soil samples were also collected from the plant sampling sites and subjected to an analysis for 18 characteristics. The minimum plant length was 69 mm and maximum 323 mm, minimum length of underground part was 29 mm, and maximum 49 mm, and the number of leaves varied between 1-10. The ecological characteristics revealed that O. lutea subsp. minor flourishes well from sea level to 800 m, and the most common habitats of O. lutea subsp. minor are rocky limestones, phrygana, macchie, olive gorves, woodland margins.Item Evaluation of linden fibre as a potential reinforcement material for polymer compositesSeki, Y; Seki, Y; Sarikanat, M; Sever, K; Durmuskahya, C; Bozaci, EThe aim of this study is to characterize linden fibres as a novel cellulose-based fibre to be used as a reinforcement material in composites and to investigate the adhesion property to unsaturated polyester. Up to now, there is no report regarding the potential usability of linden fibre in composite applications. Linden fibres were extracted from the stem of a plant of Tilia rubra DC. subsp. caucasica (Rupr.) V.Engl. Characterization of linden fibres was studied by Fourier transform infrared, X-ray photoelectron spectroscopy, thermogravimetric analysis, X-ray diffraction analysis, tensile and pull-out tests. Morphological properties of the fibres were observed through scanning electron and optical microscopes. Initial degradation temperature of the linden fibre was reported to be 238 degrees C. The tensile strength and the Young's modulus of the linden fibres were calculated to be 675.4 +/- 45.7 MPa and 61.0 +/- 9.8 GPa, respectively. The interfacial shear strength of the linden fibre with unsaturated polyester matrix was computed as 26.15 +/- 2.27 MPa via pullout test. This study offers an alternative and eco-friendly reinforcement material which may have usability potential in polymeric composites.Item Antileishmanial Activity of Selected Turkish Medicinal PlantsOzbilgin, A; Durmuskahya, C; Kayalar, H; Ertabaklar, H; Gunduz, C; Ural, IO; Zeyrek, F; Kurt, O; Cavus, I; Balcioglu, C; Toz, SO; Ozbel, YPurpose: To determine the in vitro and in vivo anti-leishmanial activities of extracts obtained from Centaurea calolepis, Phlomis lycia, Eryngium thorifolium, Origanum sipyleum and Galium incanum ssp. centrale. Methods: To estimate the cytotoxicity of plant extracts, WST-1 assay was used. Parasite inhibition in the presence of plant extracts (25 - 500 mu g/ml) in comparision with control group and reference group (glucantime, 25 mu g/ml) at 12 - 72 h were determined in vitro on L. tropica promastigotes. The in vivo leishmanicidal activity of the extracts was evaluated against L. tropica-infected mice with glucantime as reference drug. Results: The chloroform extract of Galium incanum ssp. centrale showed the highest cytotoxicity with IC50 value of 0.0316 +/- 0.005 mu g/ml. In vitro parasite inhibition by the plant extracts ranged between 16.7 +/- 0.01 % and 100 +/- 0.00 % at 25 mu g/ml concentration. The methanol extract of Eryngium thorifolium possessed the highest activity on promastigotes of L. tropica with 100 % inhibition at 25 mu g/ml. The water and chloroform extracts of C. calolepis and water and methanol extracts of E. thorifolium at a dose of 100 mg/kg reduced parasitaemia in L. tropica infected mice. Conclusion: Parasite viability results suggest that the methanol extract of Eryngium thorifolium, regarded as non-cytotoxic, is a promising candidate drug for treating L. tropica infection.Item Traditional phytotherapy and trans-cultural pharmacy among Turkish migrants living in Cologne, GermanyPieroni, A; Muenz, H; Akbulut, M; Baser, KHC; Durmuskahya, CThis article reports on an ethnopharmaceutical field study carried out among Turkish migrants in Cologne, western Germany, which recorded 79 botanical taxa and 115 plant-based preparations, encompassing 167 folk phytotherapeutical uses, as well as a few other biological (animal and mineral derived) remedies. One-fourth of the recorded remedies were represented by food-medicines. Half of the ingredients quoted came originally from Turkey; only a few plants were gathered from the wild or cultivated in the Cologne area. This article discusses the Turkish migrants' frequent use of aerial parts of Sideritis species, the fruits of Pistacia terebinthus and the seeds of Peganum harmala from the perspective of modern phytopharmacology, phytotherapy and toxicology. It considers cultural adaptation related to the use of folk pharmaceuticals by analysing the ingredients of the Turkish folk pharmacopoeia and comparing them with those of the Turkish medical ethnobotany and of the phytotherapy of the host country (Germany). Only one third of the remedies mentioned by Turkish migrants living in Cologne are also known in German evidence-based phytotherapy, and less than 10% of the recorded phytotherapeutic uses are common to both pharmacopoeias. This article concludes by suggesting hypotheses on future changes affecting the knowledge and use of medicinal plants within the Turkish community of Cologne. (C) 2005 Elsevier Ireland Ltd. All rights reserved.Item Origanum Sipyleum Methanol Extract in Combination with Ponatinib Shows Synergistic anti-Leukemic Activities on Chronic Myeloid Leukemia CellsKayabasi, C; Susluer, SY; Okcanoglu, TB; Yelken, BO; Mutlu, Z; Bagca, BG; Kurt, CC; Saydam, G; Durmuskahya, C; Kayalar, H; Ozbilgin, A; Avci, CB; Gunduz, COriganum sipyleum is used in folk medicine due to its anti-inflammatory, antimicrobial, and antioxidant properties. Ponatinib, an effective tyrosine kinase inhibitor in the treatment of chronic myeloid leukemia (CML), has severe side effects. Thus, we aimed to determine a novel herbal combination therapy that might not only increase the anti-leukemic efficacy but also reduce the dose of ponatinib in targeting CML cells. Origanum sipyleum was extracted with methanol (OSM), and secondary metabolites were determined by phytochemical screening tests. The cytotoxic effects of OSM on K562 cells were measured by WST-1 assay. Median-effect equation was used to analyze the combination of ponatinib and OSM (p-OSM). Apoptosis, proliferation, and cell-cycle were investigated by flow-cytometry. Cell-cycle-related gene expressions were evaluated by qRT-PCR. OSM that contains terpenoids, flavonoids, tannins, and anthracenes exhibited cytotoxic effects on K562 cells. The median-effect of p-OSM was found as synergistic; OSM reduced the ponatinib dose similar to 5-fold. p-OSM elevated the apoptotic and anti-proliferative activity of ponatinib. Consistently, p-OSM blocked cell-cycle progression in G(0)/G(1), S phases accompanied by regulations in TGFB2, ATR, PP2A, p18, CCND1, CCND2, and CCNA1 expressions. OSM enhanced the anti-leukemic activity of ponatinib synergistically via inducing apoptosis, suppressing proliferation, and cell-cycle. As a result, OSM might offer a potential strategy for treating patients with CML.Item Assessment of in vivo antimalarial activities of some selected medicinal plants from TurkeyOzbilgin, A; Durmuskahya, C; Kayalar, H; Ostan, IResistant infections lead to increased necessity of searching novel drugs and drug combinations. The purpose of this paper was to investigate antimalarial properties of some selected medicinal plants that have been traditionally used in Turkey for antipyretic and analgesic purposes. Lavandula stoecheas subsp. cariensis, Phlomis nissolii, Phlomis bourgaei, Phlomis leucophracta, Centaurea hierapolitana, Centaurea polyclada, Centaurea lydia, Scrophularia cryptophila, Scrophularia depauperata, Scrophularia floribunda, Rubia davisiana, and Alkanna tinctoria subsp. subleiocarpa were investigated for their in vivo antimalarial activities in mice infected with Plasmodium yoelii. Two hundred fifty to 500 mg/kg doses of plant extracts were given to mice as a single daily dose for 4 days. P. nissolii water extract, C. lydia chloroform extract, S. cryptophila ethanol extract, and C. polyclada methanol extract showed antimalarial activity with reducing parasitaemia. The chemotherapeutic effects of plant extracts ranged between 13.5 % and 66.91 %. The chemosuppressions exerted by combined plant extracts of P. nissolii, S. cryptophila, and C. lydia with C. polyclada methanol extract were detected as 51.25 %, 57.33 %, and 58.33 %, respectively. Investigation of cytotoxic activities against brine shrimps revealed that methanol extract of C. polycada, chloroform extract of C. lydia, and ethanol extract of S. cryptophila showed cytotoxic activities, while water extract of P. nissolii was not active against brine shrimps.