Browsing by Author "Eda Becer"

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    Protective Effects of Citrus Flavonoid Hesperidin in Enterocytes After Induction with TNF-α and IFN-ɣ Which Mimic the COVID-19 Disease
    (2023) Uğur Özgürbüz; Seda Vatansever; Eda Becer; HILAL KABADAYI; Damla Akoğulları Çelik
    BACKGROUND/AIMS: Coronavirus disease (COVID-19) is caused by a virus and exhibits various symptoms such as cough, fever, and chills. Flavonoids have a potential inhibitory effect on coronaviruses. In this study, we determined the effects of hesperidin on enterocyte cells (IEC) after tumor necrosis factor (TNF)-α and interferon (IFN)-γ induction which mimics the severe acute respiratory therapy-coronavirus-2 (SARS- CoV-2) infection. MATERIALS AND METHODS: The IEC-6 were treated with 50 ng/mL of TNF-α and 100 ng/mL of IFN-ɣ for 48 h to mimic inflammatory shock similar to COVID-19 disease. IEC-6 cells were cultured as control, COVID-19 disease mimic, hesperidin prophylactic, or treated groups. The cytotoxicity effect of hesperidin was analyzed using an MTT assay. Serum levels of TNF-α and interleukin (IL)8 were evaluated using ELISA. The distributions of TNF-α, IFN-ɣ, IL-1ß, Insulin-like growth factor-I, and caspase-3 were analyzed by indirect immunoperoxidase staining. RESULTS: Both TNF- α and IL8 levels were higher in TNF- α and IFN- ɣ induction of enterocyte culture medium than in the control. Lesser immunoreactivity of TNF-α was detected in the treatment group which hesperidin applicate after TNF- α and IFN-ɣ combination. While IL-1 immunoreactivity was similar in both the hesperidin prophylactic and treatment groups, lesser immunoreactivity of TNF-α was observed in the hesperidin treatment group. Both IFN-ɣ and vascular endothelial growth factor A immunoreactivities were also decreased in the hesperidin treatment group. CONCLUSION: We found that hesperidin had anti-inflammatory and cell protection effects in IEC after TNF-α and IFN-γ induction which mimics the model of SARS-CoV-2 infection. Therefore, hesperidin could be used to reduce gastrointestinal system symptoms in COVID-19 disease.
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    Kersetin Primer (Colo320) ve Metastatik (Colo741) Kolon Kanseri Hücre Dizilerinde Eksozom Salgısını ve Total miRNA Konsantrasyonunu Değiştirir
    (2023) Eda Becer; SERPİL ÖZSOY; HILAL KABADAYI; Hafize seda Vatansever
    Amaç: Kolon kanserinin önlenmesinde potansiyel bir anti-kanser ajan olarak kabul edilen kersetin, doğal polifenolik bileşiklerinden biridir. Hücrelerden ve bileşenlerinden salgılanan eksozom gibi hücre dışı veziküller, proteinleri veya miRNA’ları taşıma yoluyla hücresel davranış özelliklerine katkıda bulunur. Bu çalışmada, kersetin uygulanan Colo320 ve Colo741 kolon kanseri hücre dizilerinde Dicer, Ago2, eIF2 α CD9 ve CD63’ün sitotoksisitesini ve eksozomal miRNA salgılanması ve ekspresyonu üzerine etkilerini belirlemeyi amaçladık. Yöntemler: Kersetin sitotoksisitesinin analizi için MTT testi kullanıldı. Colo320 ve Colo741’de Dicer, Ago2, eIF2a, CD9 ve CD63’ün dağıtımı için dolaylı immünoperoksidaz boyaması kullanıldı. Eksozomdaki toplam miRNA, miRCURY™ Kit ile belirlendi. Bulgular: Kersetin uygulamasından sonra eIF2a ve CD9 immünoreaktiviteleri Colo741 kontrol grubuna kıyasla önemli ölçüde farklılık gösterdi. Ayrıca eksozomal miRNA konsantrasyonları hem kersetin uygulanan Colo320 hem de Colo741 hücrelerinde daha yüksekti. Sonuç: Kersetin uygulamasının Colo320 ve Colo741 hücrelerinde eksozomal salgılamayı tetiklediği sonucuna vardık. Bununla birlikte, primer ve metastatik kolon adenokarsinom hücrelerinde kersetin rolünü anlamak için gelecekteki araştırmalarda eksozomal bileşen değerlendirilmelidir.
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    Resveratrol modulates miRNA machinery proteins in different types of colon cancer cells
    (2024) Eda Becer; servet madencioglu; HILAL KABADAYI; Seda Vatansever; Seda Vatansever
    Objectives: Resveratrol (RSV) is a stilbenoid compound that shows anticancer activity in many cancer cells. Exosomes might affect carcinogenesis and the development of colo- rectal cancer by affecting communication between tumor cells in the tumor microenvironment via their cargo content miRNA. The aim of this study is to determine the effect of RSV on the expression of Dicer, Ago2, eIf2α, CD-9, CD-63, and exosomal miRNA levels in COLO320 and COLO741 colon cancer cell lines. Methods: The MTT method was used for cell growth and cytotoxicity in both COLO320 and COLO741 cell lines. Dicer, Ago2, eIF2α, CD-9, and CD-63 antibodies were used for the immunocytochemical evaluation. Total miRNA analysis was performed using a miRCURY Exosome Isolation Kit. Results: As a result of immunocytochemical staining, increased CD-63 immunoreactivity was observed in RSV-treated COLO320 cells vs. RSV-treated COLO-741 cells. Dicer immunoreactivity increased after the RSV treatment in COLO320 cells. Higher eIF2α immunoreactivity was observed in RSV-treated COLO741 cells compared to both COLO741 control cells and RSV-treated COLO320 cells. Non- significant decreases were observed in miRNA concentra- tion in RSV-treated COLO320 and COLO741 cells compared to control group cells. Conclusions: RSV could increase miRNA biogenesis in COLO320 cancer cells and decrease it in COLO741 cancer cells.