Browsing by Author "El, S"

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    Evaluation of Real-Time PCR Method for Rapid Diagnosis of Brucellosis with Different Clinical Manifestations
    Surucuoglu, S; El, S; Ural, S; Gazi, H; Kurutepe, S; Taskiran, P; Yurtsever, SG
    In this study, we tested the advantages of TaqMan real time PCR technique and compare it to conventional methods using serum samples from patients with different clinical forms of brucellosis. A total of 50 patients were included in the study. Blood culture using BACTEC 9240 system, Standard Wright's tube agglutination, and real time PCR methods were used. Control blood samples from 30 people with no history of brucellosis or exposure to Brucella spp. were examined too. Serological assay was positive for 49 patients (98%). Forty-four (88%) of the 50 patients had a positive PCR result, whereas Brucella spp were isolated from blood cultures of 18 patients (36%). STA test was all positive for focal brucellosis. Real time PCR test was positive in 9 patients with focal disease (90%), whereas blood culture was positive only in 4 patients (40%). The sensitivity, specificity, positive and negative predictive values of the real time PCR method were calculated as 88%, 100%, 100%, and 83%, respectively. Our results suggest that the high sensitivity and specificity of real time PCR method make it a useful tool for diagnosis of brucellosis with different clinical manifestations.
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    Significance of Fluorescent In-Situ Hybridization Method in Rapid Diagnosis of Brucellosis
    Gazi, H; Yurtsever, SG; Sürücüoglu, S; El, S; Ertural, P
    Objective: Brucellosis is a zoonosis commonly seen worldwide it causes severe disease and great economic loss. Rapid and sensitive tests are increasingly required for detection of the disease since currently used conventional diagnostic methods give results late and there are some factors affecting its sensitivity. Recently, a number of diagnostic tests have been determined that use different clinical samples in order to diagnose brucellosis in a short time. However a sufficiently sensitive and specific, optimized, routinely applicable molecular method is still lacking for detection of brucellosis which is defined as a difficult infection in terms of diagnosis and treatment. Fluorescent in situ hybridization (FISH) is a novel molecular diagnostic method that can detect Brucella spp. from hemoculture tubes signaling positively by shortening identification time. In this study, we aimed to compare FISH technique with conventional culture method in diagnosis of brucellosis and to investigate its use routinely. Material and Methods: A total of 100 hemoculture samples of patients who applied with the suspicion of brucellosis were studied with FISH and culture methods concurrently, after gram staining. Standard Wright's tube agglutination (STA) test was also used for interpretation of the FISH results. Results: Of the tested samples, 43 studied with cultures, 52 with STA and 44 with FISH were found positive. The FISH detected Brucella spp. in 34 positive cultures and in 29 STA positive sample. Sensitivity, specifity, positive and negative predictive values of FISH method were found as 79.0%, 82.4%, 77.2% and 83.9% respectively when evaluated together with culture which is the gold standard method. Conclusion: FISH is a rapid and easily applicable method not requiring much equipment. However, it was concluded that it could not be used as a cost-effective diagnostic tool as it was not as sensitive enough as desired, and in case of it is used, it would be useful to confirm FISH negative samples with culture and/or serum tube agglutination test.
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    Evaluation of Serological Tests for Diagnosis of Brucellosis
    Pabuccuoglu, O; Ecemis, T; El, S; Coskun, A; Akcali, S; Sanlidag, T
    The aim of the present study was to compare serological tests (Rose Bengal [RB]; standard agglutination test [SAT]; enzyme immunoassay [ETA] for detection of IgM, IgA, and IgG; and 2-mercaptoethanol [2-ME] test) that are routinely used in patients prediagnosed with different clinical types of brucellosis (acute, subacute, or chronic), and to evaluate the results of the IgG avidity test. Ninety-two patients having titers >= 1/160 as measured by SAT were included in the study. The IgG avidity test was performed in 78 patients who had positive EIA-IgG results. RB test results were positive in 88 (95.7%) patients. A statistically significant correlation was found between a positive EIA-IgM result and the diagnosis of acute brucellosis. When compared to the results of the SAT, the 2-ME test showed a lower titer in 55 (59.8%) patients, and the agreement between the 2-ME test and EIA-IgG was calculated as 84.8%. No statistical difference was found between the 40% avidity index used in the IgG avidity test and avidity maturation time (6 months). From our study, we concluded that (i) the RB and SAT tests are appropriate and reliable tests for the serological diagnosis of brucellosis; (ii) IgM can be used as a marker of acute brucellosis; (iii) the 2-ME test, similar to EIA, can be used to determine IgM levels; and (iv) the IgG avidity test should be standardized.