Browsing by Author "Elfalleh W."

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    Datura innoxia and Dipsacus laciniatus: Biological activity and phenolic composition
    (Elsevier Ltd, 2019) Benabderrahim M.A.; Sarikurkcu C.; Elfalleh W.; Ozer M.S.
    In the present study, we investigated the phenolic composition of the methanol extract from the aboveground of two plants of Turkish flora; Datura innoxia and Dipsacus laciniatus. In addition, the antioxidant (phosphomolybdenum, CUPRAC, FRAP, DPPH, ABTS)and enzyme inhibitory activities (α-amylase and tyrosinase), and total phenolic and flavonoids contents were determined in these plant extracts. The results showed high significant differences among plants (p ≤ 0.05)for the antioxidant capacities measured with the different methods, the enzyme inhibitory activities, and the all identified phenolic compounds except for hyperoside. From a total of 23 identified phenolic compounds, 19 were found in both plants. The main flavonoids identified were (+)-catechin and (−)-epicatechin and hyperoside. (+)-Catechin and (−)-epicatechin showed very high concentrations in D. innoxia (12937.39 ± 108.86 and 24147.64 ± 2512.35 μg/g of dry plant, respectively)and in D. laciniatus (4947.99 ± 14.18 and 13171.30 ± 2410.76 μg/g of dry plant, respectively). Hyperoside had interesting contents with comparable values (115.11 ± 16.20 in D. innoxia, and 110.77 ± 16.32 μg/g of dry plant in D. laciniatus). The major phenolic acid was chlorogenic acid especially in D. laciniatus (12124.22 ± 598.13 μg/g of dry plant). These compounds were correlated to the high antioxidant and enzyme inhibitory activities for both species and confirm their medicinal traditional uses. Hence, further screening of bioactive compounds in different organs such as flowers and roots of these plants is crucial for the discovering of new source of natural antioxidants and enzyme inhibitors. © 2019 Elsevier Ltd
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    Phenolic composition and biological activities of Turkish endemic plant: Stachys cretica subsp. kutahyensis
    (Elsevier B.V., 2021) Benabderrahim M.A.; Sarikurkcu C.; Elfalleh W.; Ozer M.S.; Ceylan O.
    Stachys cretica L. subsp. kutahyensis is a Turkish endemic plant specie and used in flock medicine against many diseases. To our knowledge, no data are available on the chemical composition and the biological activities of this plant. In this study, the phytochemical composition, the enzyme inhibition and antioxidant activity of this specie were assessed. The Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC–ESI–MS) analysis allowed the determination of 26 phenolic compounds with predominance of chlorogenic acid and verbascoside. The concentration of phenolic acids and flavonoid compounds significantly changed according to solvent used (water and methanol). From five different methods used, the antioxidant activity was important and significantly different between extracts for ferrous ion chelating and ABTS assays where the aqueous extract expressed higher antioxidant activity. However, interesting enzyme inhibition activities were observed for the extracts of S. cretica subsp. kutahyensis using α-amylase and tyrosinase. The highest α-amylase and tyrosinase inhibition activities were recorded with the methanol extract. The results confirm that S. cretica subsp. kutahyensis could be used as valuable new natural source with beneficial biological properties for food and pharmacology applications. © 2020 SAAB