Browsing by Author "Elibol, M"

Now showing 1 - 6 of 6
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    Microalgae pigments as a sustainable approach to textile dyeing: A critical review
    Mutaf-Kilic, T; Demir, A; Elibol, M; Oncel, SS
    Microalgae derived pigments, such as phycobiliproteins, chlorophylls, and carotenoids, offer eco-friendly al-ternatives to synthetic dyes. These pigments are known for their vibrant colors, as well as their antimicrobial, antioxidative, and UV protective properties, making them commonly used in the food, pharmaceutical, and cosmetic industries. Recently, they have also gained attention in the textile dyeing industry. Compared to other natural dye sources like plants, bacteria, and fungi, microalgae pigments possess significant potential as natural textile dyes. They exhibit high pigment productivity, more sustainable production systems, current industrial-scale production, and a lack of seasonal dependency. This review aims to provide an overview of the current state, limitations, and future prospects regarding the utilization of microalgae pigments as textile dyes. The report emphasizes the commercial availability of microalgae pigments, highlights current studies exploring their dyeing potential on various textile materials, and discusses the development techniques aimed at enhancing dyeing performance. This emerging and promising research field has the potential to revolutionize the pro-duction of eco-friendly and sustainable textile products, while also contributing to a reduction in the use of synthetic dyes in the textile industry.
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    Exploring deep eutectic solvents for enhanced extraction of bio-active compounds from microalgae biomass
    Ozel, N; Inam, A; Elibol, M
    Microalgae biomass contains proteins, polysaccharides, carotenoids, phenolic compounds, vitamins, minerals, and other valuable compounds. In the extraction process of useful intracellular products from biomass, deep eutectic solvents (DESs) offer several advantages as an alternative to organic solvents. This study used deep eutectic solvents as extraction media to obtain bio-active compounds from freeze-dried microalgae biomass. This study aimed to evaluate the effect of two different DESs (Choline chloride: acetic acid (1:2) (CCA) and Choline chloride: urea (1:2) (CCU)) on the extraction of lipids, carotenoids, proteins, phenolic compounds, and carbohydrates from different microalgae biomass including Schizochytrium sp., Chlorella vulgaris, Scenedesmus protuberans and Neochloris texensis. The best results were obtained as follows: proteins from CCA-N. texensis (28.99 mg/g DW), carotenoids from CCA-S. protuberans (33.55 mg/g DW), phenolics from CCA-S. protuberans (33.44 mg GAE/g DW), carbohydrates from CCA-S. protuberans (18.32 mg/g DW), and lipids (the treated biomass pellets) from CCU-N. texensis (18.3 %). UV-spectrophotometry, LC-MS/MS, and GC-MS were used to analyse the carotenoid, phenolic, and lipid content, respectively.
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    Green Synthesis and Characterization of Titanium Nanoparticles Using Microalga, Phaeodactylum tricornutum
    Caliskan, G; Mutaf, T; Agba, HC; Elibol, M
    Nanoparticles synthesized from microalgae offer a newly discovered process that is open to improvement. In this study supernatant of Phaeodactylum tricornutum (P. tricornutum) culture was used for this purpose. Firstly, the effects of some variables, namely titanium concentrations, titanium:supernatant ratio, pH, incubation time, and mixing speed on nanoparticle synthesis were investigated by using statistical design method in shaken culture as well as bioreactor. The average nanoparticle size synthesized in optimum conditions which were pH 7.5 with 300 rpm for 1 h was found as 50 nm. In the second part, nanoparticles were coated with a chitosan solution to protect their stability and increase their potential. Therefore, the antimicrobial activity showed a reasonable effect on these nanoparticles coated with chitosan. Nanoparticles produced had interestingly shown 99% antistatic properties. According to the cytotoxicity test, these nanoparticles showed a high cytotoxic effect on different cancer cell lines. The results obtained in the present study can be considered promising outcomes for possible future antimicrobial, biogenic and antistatic studies particularly in biomedical applications.
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    Green synthesis of titanium nanoparticles using a sustainable microalgal metabolite solution for potential biotechnological activities
    Mutaf, T; Caliskan, G; Ozel, H; Akagac, G; Öncel, SS; Elibol, M
    In this study, green synthesis of titanium nanoparticles using liquids metabolites of microalgae, Porphyridium cruentum, was performed to evaluate potential biotechnological activity. The rising rates of multidrug-resistant bacteria and the number of cancer patients are driving the search for novel antimicrobial and anticancer agents to combat this threat. In recent years, with the increasing number of studies, nanomaterials are starting to be better understood and are emerging as a solution to this problem. Especially, green synthesized nanoparticles with anticancer, antioxidant, and antimicrobial activities have potential in biomedical applications because of their eco-friendly and biocompatible nature. Scanning electron microscopy (SEM) images revealed that spherical shaped Ti-NPs' size ranged from 62 to 133 nm. This study aimed to assess the effectiveness of antibacterial activity of Ti-NPs and chitosan-coated Ti-NPs against Escherichia coli and Staphylococcus aureus using disc diffusion assay. It demonstrated the concentration-dependent cytotoxic effect of Ti-NPs of human prostate adenocarcinoma (PC-3), human alveolar adenocarcinoma (A549), and human mammary gland adenocarcinoma (MDA-MB) cancer cell lines. This present study shows promising outcomes for possible future applications of synthesized Ti-NPs as a novel antibacterial and cytotoxic agent for biomedical applications such as drug delivery, biosensor, and hyperthermia.
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    Green synthesis of metal nanoparticles by microalgae
    Mutaf, T; Caliskan, G; Öncel, SS; Elibol, M
    Green synthesis of metal nanoparticles through biological resources has attracted attention in recent years. The main reason for that, a lot of toxic chemicals as reducing and stabilizing agents are used in physical and chemical methods which are known as conventional methods. Organisms such as plants, fungi, bacteria, and algae are alternative sources for green nanoparticle synthesis because of their more eco-friendly nature and not be a threat to human health. Microalgae as aquatic microorganisms have been added into the formulations of food, cosmetics, and pharmaceutical for many years, due to their high value-added metabolites such as proteins, vitamins, pigments, fatty acids, intracellular and extracellular polysaccharides. In addition, microalgae have a high potential in biogenic nanoparticle synthesis because of their metal ions accumulation capability, phytoremediation potential, and rich in intracellular and extracellular metabolites that will reduce metal ions to elemental state. In recent years, the number of studies, focused on silver, gold, titanium, zinc, iron, etc. nanoparticle synthesis from many microalgae species by intracellular and extracellular pathways has increased. This review article aims to provide a brief outline of microalgae and cyanobacteria species studied in the context of nanoparticle synthesis, different approaches for nanoparticle synthesis from microalgae, the mechanism of nanoparticle synthesis, and basic characterization principles and antimicrobial activities of nanoparticles produced by green synthesis.
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    Modification of chitosan-bead support materials with l-lysine and l-asparagine for α-amylase immobilization
    Yazgan, I; Turner, EG; Cronmiller, LE; Tepe, M; Ozturk, TK; Elibol, M
    Maltose syrups have got wide-range utilizations in a variety of applications from bakery to drug-development. alpha-Amylases are among the most widely utilized industrial enzymes due to their high specificity in production of maltose syrup from starch. However, enzymes are not stable in ex vivo conditions towards alteration in pH, temperature, and such other parameters as high salt concentrations and impurities, where immobilization is required to advance the stability of the enzyme with which approach the requirement of isolation of the enzyme from media is eliminated as well. In this study, Termamyl(A (R)) alpha-amylase was immobilized on the none-modified chitosan beads (NMCB), l-lysine-modified chitosan beads (LMCB), and l-asparagine-modified chitosan beads (AMCB) to assess effects of the support material on optimum conditions and kinetic parameters of the alpha-amylase activity in production of maltose from starch. Immobilization on NMCB, LMCB, and AMCB puts a strong influence on optimum pH, optimum temperature, stability, and kinetic parameters of alpha-amylase. Modification of chitosan beads with l-lysine and l-asparagine dramatically altered the overall immobilization yield, and enzyme's response to pH and temperature variations and the kinetic parameters. AMCB provided the best immobilization yield (49%), while LMCB only improved the yield by 2% from 22 to 24%.