Browsing by Author "Ellidokuz, E"

Now showing 1 - 11 of 11
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    A novel concept: occult HBV infection in the western region of Turkey
    Akduman, E; Sanlidag, T; Akcali, S; Ellidokuz, E; Celebi Kobak, A
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    ALTERATIONS OF COPY NUMBER OF METHYLATION PATTERN IN MISMATCH REPAIR GENES BY METHYLATION SPECIFIC-MULTIPLEX LIGATION-DEPENDENT PROBE AMPLIFICATION IN CASES OF COLON CANCER
    Onrat, ST; Ceken, I; Ellidokuz, E; Kupelioglu, A
    Genetic alterations and changes in genomic DNA cytosine methylation patterns are associated with all types of cancer and are caused by germline mutations in DNA mismatch repair (MMR) genes, predominantly MLH1 (MutL homolog 1, 19 exons) and MSH2 (MutS homolog 2, 16 exons). Genomic DNA was extracted from tissue samples embedded in paraffin from 49 patients with adenocarcinoma and from 21 patients with carcinoma for the study group; genomic DNA was extracted from lymphocytes from 10 healthy donors for the control group. We used methylation specific multiplex ligation-dependent probe amplification (MS-MLPA), which allows the detection of copy number changes and unusual methylation levels of 10 to 50 different sequences in one reaction by use of the methylation-sensitive restriction enzyme HhaI and sequence-specific capillary electrophoresis for the study of 24 genes. We found the mean methylation rates for MLH1 (97.14%), MSH2 (24.28%), MSH6 (MutS homolog 6) (67.14%), MSH3 (MutS homolog 3) (78.57%), MLH3 (MutL homolog 3) (75.71%), PMS2 (postmeiotic segregation increased 2) (65.71%), MGMT(O-6-methylguanine-DNA methyltransferase) (82.85%). We conclude that the mismatch repair (MMR) system is critical for the maintenance of genomic stability.
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    Transient postpartum diabetes insipidus associated with HELLP syndrome
    Ellidokuz, E; Uslan, I; Demir, S; Cevrioglu, S; Tufan, G
    Diabetes insipidus in pregnancy has different causes. The association of diabetes insipidus with disturbances of liver function has been reported, however, diabetes insipidus has rarely been reported in HELLP syndrome. We present a 23-year-old primigravida with a singleton gestation complicated by HELLP syndrome who developed postpartum diabetes insipidus. Labor was induced promptly to terminate pregnancy because of intrauterine fetal death and liver dysfunction. 1-deamino-8-D-arginine-vasopressin was administered. Diabetes insipidus and liver dysfunction resolved within 2 weeks. Development of diabetes insipidus may result from increased vasopressinase activity mainly caused by deterioration of liver functions caused by HELLP syndrome. In pregnant women with liver disease as a result of any cause, the development of diabetes insipidus should be assessed with particular attention.
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    Gastric polypoid intramucosal carcinoma and an adjacently located leiomyoma at the cardia
    Kobak, AÇ; Zeybel, M; Ayhan, S; Aydin, A; Kaya, Y; Ellidokuz, E
    We report a 65-year-old patient with a gastric polyp of 2.5 cm in diameter located at the cardia on upper gastrointestinal (GI) endoscopy. Pathological examination of the excised polyp showed intramucasal carcinoma. Endoscopic ultrasonography (EUS) reported the lesion as early gastric carcinoma with probable submucosal involvement. On serial sections of the gastrectomy material, the lesion was an intramucasal carcinoma and surprisingly there was a leiomyoma located adjacently.
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    Role of the PON polymorphisms on progression of chronic hepatitis and cirrhosis
    Aldirmaz, M; Altintas, N; Var, A; Ellidokuz, E
    Aim and Background: The probable role of PON1-192, PON1-55, PON2-148, PON2-311 polymorphisms of the PON enzyme family, which are play a role in antioxidant pathways in the progression of chronic hepatitis to cirrhosis was investigated. Methods: The patient population included 64 chronic hepatitis patients without cirrhosis and 30 patients with cirrhosis which were diagnosed by biopsy compared to a control group (n=68) of normal healthy volunteers. All patients were recruited from the outpatient clinic of Gastroenterology, Celal Bayar University Faculty of Medicine. Genomic DNA of leukocytes was isolated by using a commercial isolation kit. PCR analysis was assessed for PON1-192, PON1-55, PON2-148, PON2-311 genotypes and the products digested with HinfI restriction enzyme to see the allelic polymorphisms. The PCR products were resolved on electrophoresis 2% agarose gel and visualized with a Syngene (USA) image analysis system. Odds ratios in 95% confidence interval were calculated for the two genotypes. For the statistical analyses SPSS version 10.0 was used. Results: The prevalence of PON1-192, PON1-55, PON2-148 genotypes were very different between the groups but was not statistically significant. The frequency of the SS and SC genotypes of PON2-311 in patients with hepatitis and cirrhosis was higher than the control group and their Odds Ratios were statistically significant. Conclusion: The prevalence of PON2 gene SS and SC genotypes of in patients was higher than that of healthy volunteers (ORs were 3,855 and 2,404 respectively). Similarly SS and SC genotypes of in patients with cirrhosis was higher than that of patients with hepatitis (ORs were 3,436 and 2,223 respectively) These results suggested that the SS and SC genotypes of PON2 gene might cause a susceptibility for developing of hepatitis and progression of this condition to cirrhosis. It can be also speculated that CC genotype may be protective for the progression of disease.
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    Never in mitosis gene A-related kinase 6 and aurora kinase A: New gene biomarkers in the conversion from ulcerative colitis to colorectal cancer
    Gerçeker, E; Boyacioglu, SO; Kasap, E; Baykan, A; Yuceyar, H; Yildirim, H; Ayhan, S; Ellidokuz, E; Korkmaz, M
    Ulcerative colitis (UC) is an important risk factor for colorectal cancer (CRC). Histone modifications are one of the epigenetic mechanisms that may have key roles in the carcinogenesis of CRC. At present, there are no studies comparing histone modification patterns of UC and CRC in the literature. Therefore the aim of the present study was to investigate whether genes, particularly those involved in histone modification, have value in patient monitoring with regards to CRC development in UC. Key gene expressions of the histone modification enzyme were assessed and compared in CRC, UC and control groups using the RT-PCR array technique. Patients were divided into subgroups based on the extent and duration of the disease and inflammatory burden, which are considered risk factors for CRC development in UC patients. In UC and CRC groups, a significantly higher overexpression of the NEK6 and AURKA genes compared to the control group was identified. In addition, there was a significantly higher overexpression of HDAC1 and PAK1 genes in the UC group, and of HDAC1, HDAC7, PAKI and AURKB genes in the CRC group. NEK6, AURKA, HDAC1 and PAK1 were significantly overexpressed in patients with a longer UC duration. Overexpression of AURKA and NEK6 genes was significantly more pronounced in UC patients with more extensive colon involvement. HDAC1, HDAC7, PAK1, NEK6, AURKA and AURKB are important diagnostic and prognostic markers involved in the carcinogenesis of CRC. HDAC1, PAK1, NEK6 and AURKA may be considered as diagnostic markers to be used in CRC screening for UC patients.
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    COEXISTENCE OF SYMPTOMATIC IRON-DEFICIENCY ANEMIA AND DUODENAL NODULAR LYMPHOID HYPERPLASIA DUE TO GIARDIASIS: Case Report
    Kasrga, E; Gülen, H; Simsek, A; Ayhan, S; Ylmaz, Ö; Ellidokuz, E
    Iron-deficiency anemia due to iron malabsorption and duodenal nodular lymphoid hyperplasia (NLH) has been described in children with Giardia intestinalis infection. Also, symptomatic iron-deficiency anemia is rarely encountered in male adolescents. A 14-year-old boy underwent esophagogastroduodenoscopy for investigation of symptomatic iron-deficiency anemia (hemoglobin 5.8 g/dL, mean corpuscular volume 65.3 fL, serum ferritin 1.5 ng/mL). He had a sufficient diet for iron and recurrent bouts of diarrhea without melena. At upper endoscopy, duodenal mucosa was diffusely nodular. Histopathologic evaluation of biopsy samples from the duodenum revealed infection with Giardia intestinalis. His anemia improved with metronidazole and iron treatment.
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    Are RHOA, CSNK1A1, DVL2, FZD8 and LRP5 Genes Novel Biomarkers in the conversion from Intestinal Metaplasia to Gastric Cancer?
    Demirci, U; Boyacioglu, SÖ; Kasap, E; Bilgiç, F; Gerçeker, E; Yildirim, H; Baykan, A; Ellidokuz, E; Yüceyar, M; Korkmaz, M
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    Nutritional changes and effects in hospitalized patients
    Yildirim, A; Ellidokuz, H; Ellidokuz, E; Ozer, ZC
    Objective: To determine the effect of hospitalization in the internal disease clinics on nutrition variables. Method: This study was a cohort-type study performed in the Internal Diseases Clinics, University Hospital in Turkey. We included 208 patients who were hospitalized in the Internal Diseases Clinics of the University between June and August 2003. The clinical nutrition parameters of all the patients were evaluated from anthropometric measurements and laboratory results at admission and discharge. Results: Of 208, 105 were females, and 103 were males. The average age was 57 +/- 13.5 (18-85) years. Average hospitalization period of the cases was 14 +/- 10 (1-73) days. While the average body weight at admission was 71.6 +/- 10.9 kg and it was found to be 70.7 +/- 1.3 kg at discharge (paired sample t test, p < 0.001). We noted the statistically significant decrease in the body mass index, waist and hip measures, muscle-skin folds thickness, and body adipose mass (p < 0.05). Decreases were observed in all the clinical parameters of laboratory test results of the patients indicating end products of fat, protein, and carbohydrate metabolism (p < 0.05). It was observed that the demographic characteristics of the patients (age, gender, occupation, education, and so forth) did not affect the decrease in nutritional parameters (p > 0.05). Conclusion: It was observed that decreases occurred in all the nutrition parameters of the patients who were hospitalized in the internal diseases clinics. It is suggested that these decreases are related to entire fat, protein, and carbohydrate metabolism. Nutritional parameters of patients should be followed during hospitalization with the purpose of preventing regression in nutrition parameters.
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    Activation of peripheral opioid receptors has no effect on heart rate variability
    Ellidokuz, E; Kaya, D; Uslan, I; Celik, A; Esen, AM; Barutca, I
    Opioid receptors involved in regulating the motility of the gastrointestinal tract have been localized in both contractile and neuronal tissues. Trimebutine, a peripheral opioid receptor agonist, modulates gastrointestinal motor activity in both directions and also may act on cardiac tissue. This study investigated the effects of trimebutine in clinical doses on cardiac autonomic functions with heart rate variability. The effect of trimebutine on cardiac autonomic outflows was evaluated in 11 healthy subjects. Trimebutine (200 mg) or placebo was administered orally at random in a double-blind, cross-over manner. Continuous electrocardiography recordings were obtained before and after drug administration during three states: rest, controlled breathing, and a hand grip exercise. Heart rate variability analysis showed that there was no significant difference between subjects administered with placebo or trimebutine throughout rest, controlled breathing, or the hand grip exercise. We concluded that trimebutine, in clinical doses, has no significant effect on cardiac autonomic functions.