Browsing by Author "Ellidokuz E."
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Item Endoscopic submucosal resection of a rectal carcinoid tumor by cap aspiration - Snare resection method(2006) Çelebi Kobak A.; Zeybel M.; Ayhan S.; Kara E.; Ellidokuz E.We report a 55-year-old man with a white plaque-like lesion 4 mm in diameter located in the rectum on colonoscopic examination. Biopsy specimens showed carcinoid tumor. Endoscopic submucosal resection (ESMR) of the lesion was successfully performed by using cap aspiration-snare resection method.Item Transient postpartum diabetes insipidus associated with HELLP syndrome(2006) Ellidokuz E.; Uslan I.; Demir S.; Cevrioglu S.; Tufan G.Diabetes insipidus in pregnancy has different causes. The association of diabetes insipidus with disturbances of liver function has been reported, however, diabetes insipidus has rarely been reported in HELLP syndrome. We present a 23-year-old primigravida with a singleton gestation complicated by HELLP syndrome who developed postpartum diabetes insipidus. Labor was induced promptly to terminate pregnancy because of intrauterine fetal death and liver dysfunction. 1-deamino-8-D-arginine-vasopressin was administered. Diabetes insipidus and liver dysfunction resolved within 2 weeks. Development of diabetes insipidus may result from increased vasopressinase activity mainly caused by deterioration of liver functions caused by HELLP syndrome. In pregnant women with liver disease as a result of any cause, the development of diabetes insipidus should be assessed with particular attention. © 2006 The Authors.Item Nutritional changes and effects in hospitalized patients(2006) Yildirim A.; Ellidokuz H.; Ellidokuz E.; Ozer Z.C.Objective: To determine the effect of hospitalization in the internal disease clinics on nutrition variables. Method: This study was a cohort-type study performed in the Internal Diseases Clinics, University Hospital in Turkey. We included 208 patients who were hospitalized in the Internal Diseases Clinics of the University between June and August 2003. The clinical nutrition parameters of all the patients were evaluated from anthropometric measurements and laboratory results at admission and discharge. Results: Of 208, 105 were females, and 103 were males. The average age was 57 ± 13.5 (18-85) years. Average hospitalization period of the cases was 14 ± 10 (1-73) days. While the average body weight at admission was 71.6 ± 10.9 kg and it was found to be 70.7 ± 1.3 kg at discharge (paired sample t test, p<0.001). We noted the statistically significant decrease in the body mass index, waist and hip measures, muscle-skin folds thickness, and body adipose mass (p<0.05). Decreases were observed in all the clinical parameters of laboratory test results of the patients indicating end products of fat, protein, and carbohydrate metabolism (p<0.05). It was observed that the demographic characteristics of the patients (age, gender, occupation, education, and so forth) did not affect the decrease in nutritional parameters (p>0.05). Conclusion: It was observed that decreases occurred in all the nutrition parameters of the patients who were hospitalized in the internal diseases clinics. It is suggested that these decreases are related to entire fat, protein, and carbohydrate metabolism. Nutritional parameters of patients should be followed during hospitalization with the purpose of preventing regression in nutrition parameters.Item Activation of peripheral opioid receptors has no effect on heart rate variability(2008) Ellidokuz E.; Kaya D.; Uslan I.; Çelik A.; Esen A.M.; Barutça I.Opioid receptors involved in regulating the motility of the gastrointestinal tract have been localized in both contractile and neuronal tissues. Trimebutine, a peripheral opioid receptor agonist, modulates gastrointestinal motor activity in both directions and also may act on cardiac tissue. This study investigated the effects of trimebutine in clinical doses on cardiac autonomic functions with heart rate variability. The effect of trimebutine on cardiac autonomic outflows was evaluated in 11 healthy subjects. Trimebutine (200 mg) or placebo was administered orally at random in a double-blind, cross-over manner. Continuous electrocardiography recordings were obtained before and after drug administration during three states: rest, controlled breathing, and a hand grip exercise. Heart rate variability analysis showed that there was no significant difference between subjects administered with placebo or trimebutine throughout rest, controlled breathing, or the hand grip exercise. We concluded that trimebutine, in clinical doses, has no significant effect on cardiac autonomic functions. © 2008 Springer.Item Gastric polypoid intramucosal carcinoma and an adjacently located leiomyoma at the cardia(2008) Çelebi Kobak A.; Zeybel M.; Ayhan S.; Aydin A.; Kaya Y.; Ellidokuz E.We report a 65-year-old patient with a gastric polyp of 2.5 cm in diameter located at the cardia on upper gastrointestinal (GI) endoscopy. Pathological examination of the excised polyp showed intramucosal carcinoma. Endoscopic ultrasonography (EUS) reported the lesion as early gastric carcinoma with probable submucosal involvement. On serial sections of the gastrectomy material, the lesion was an intramucosal carcinoma and surprisingly there was a leiomyoma located adjacently.Item Coexistence of symptomatic iron-deficiency anemia and duodenal nodular lymphoid hyperplasia due to giardiasis: Case report(2009) Kasirga E.; Gülen H.; Şimşek A.; Ayhan S.; Yilmaz Ö.; Ellidokuz E.Iron-deficiency anemia due to iron malabsorption and duodenal nodular lymphoid hyperplasia (NLH) has been described in children with Giardia intestinalis infection. Also, symptomatic iron-deficiency anemia is rarely encountered in male adolescents. A 14-year-old boy underwent esophagogastroduodenoscopy for investigation of symptomatic iron-deficiency anemia (hemoglobin 5.8 g/dL, mean corpuscular volume 65.3 fL, serum ferritin < 1.5 ng/mL). He had a sufficient diet for iron and recurrent bouts of diarrhea without melena. At upper endoscopy, duodenal mucosa was diffusely nodular. Histopathologic evaluation of biopsy samples from the duodenum revealed infection with Giardia intestinalis. His anemia improved with metronidazole and iron treatment. Copyright © Informa Healthcare USA, Inc.Item P53 intronic variant G13964C analyses in cases with colon cancer(2009) Onrat S.T.; Ellidokuz E.; Küpelioǧlu A.; Durhan E.Nucleotide alterations in p53 intron 6 have been reported to be associated with the dysregulation of p53 function and tumor development. G13964C base change functioned as dominant mutation similar to the more common missense, nonsense and splice-site mutations. To detect the G13964C variant PCR-RFLP assay was used. In this study, DNA was isolated from colon cancer tissue samples of 35 cases (19 female and 16 male) diagnosed to be colon carcinoma. In this study, we found that mutations were present in 30 (85.7%) of 35 cases enrolled into study. In 7 (23.3%) cases G/G, 21 (70.0%) cases G/C and 2 (6.7%) C/C genotypes were found. In 5 (14.3%) cases DNA isolation could not be obtained. Our results indicate that heterozygotes for the GC allele have higher frequency than other alleles and one of the reasons of colon cancer may be related to GC allele frequency.Item Frequency of TP53 codon72 polymorphism in cases with colon cancer(2009) Onrat S.T.; Ellidokuz E.; Küpelioǧlu A.; Durhan E.Recent studies indicated that the Arg allele is preferentially mutated and retained in various human cancers arising in Pro/Arg heterozygotes and it may be an important biomarker in colon cancer prognosis. In this study, DNA was isolated from paraffine-embedded colon tumor tissue samples of 35 cases diagnosed as colon carcinoma. We have observed PCR-RFLP genotyping for the codon 72 exon 4 polymorphism (Arg72Pro) of the p53 gene. In this study, we detected that TP53 codon 72 polymorphism was present in 27 (77.1%) of 35 cases enrolled into the study. In 14 (51.9%) cases Arg/Arg, 11 (40.7%) cases Arg/Pro and 2 (7.4%) cases Pro/Pro genotype frequencies were found. In 8 (22.9%) cases DNA isolation were not obtained. Our results point out that individuals homozygous for the Arg allele have higher frequency than other alleles and that colon cancer may be related to Arg allele frequency.Item Alterations of copy number of methylation pattern in mismatch repair genes by methylation specific-multiplex ligation-dependent probe amplification in cases of colon cancer(2011) Onrat S.T.; Çeken I.; Ellidokuz E.; Kupelioǧlu A.Genetic alterations and changes in genomic DNA cytosine methylation patterns are associated with all types of cancer and are caused by germline mutations in DNA mismatch repair (MMR) genes, predominantly MLH1 (MutL homolog 1, 19 exons) and MSH2 (MutS homolog 2, 16 exons). Genomic DNA was extracted from tissue samples embedded in paraffin from 49 patients with adenocarcinoma and from 21 patients with carcinoma for the study group; genomic DNA was extracted from lymphocytes from 10 healthy donors for the control group. We used methylation specific multiplex ligation-dependent probe amplification (MS-ML-PA), which allows the detection of copy number changes and unusual methylation levels of 10 to 50 different sequences in one reaction by use of the methylationsensitive restriction enzyme HhaI and sequence-specific capillary electrophoresis for the study of 24 genes.We found the mean methylation rates for MLH1 (97.14%), MSH2 (24.28%), MSH6 (MutS homolog 6) (67.14%), MSH3 (MutS homolog 3) (78.57%), MLH3 (MutL homolog 3) (75.71%), PMS2 (postmeiotic segregation increased 2) (65.71%), MGMT(O-6-methylguanine-DNA methyltransferase) (82.85%). We conclude that the mismatch repair (MMR) system is critical for the maintenance of genomic stability.Item Role of the PON polymorphisms on progression of chronic hepatitis and cirrhosis; [Kronik hepatit ve siroz gelişiminde PON polimorfizmlerinin rolü](2011) Aldirmaz M.; Altintaş N.; Var A.; Ellidokuz E.Aim and Background: The probable role of PON1-192, PON1-55, PON2-148, PON2-311 polymorphisms of the PON enzyme family, which are play a role in antioxidant pathways in the progression of chronic hepatitis to cirrhosis was investigated. Methods: The patient population included 64 chronic hepatitis patients without cirrhosis and 30 patients with cirrhosis which were diagnosed by biopsy compared to a control group (n=68) of normal healthy volunteers. All patients were recruited from the outpatient clinic of Gastroenterology, Celal Bayar University Faculty of Medicine. Genomic DNA of leukocytes was isolated by using a commercial isolation kit. PCR analysis was assessed for PON1-192, PON1-55, PON2-148, PON2-311 genotypes and the products digested with HinfI restriction enzyme to see the allelic polymorphisms. The PCR products were resolved on electrophoresis 2% agarose gel and visualized with a Syngene (USA) image analysis system. Odds ratios in 95% confidence interval were calculated for the two genotypes. For the statistical analyses SPSS version 10.0 was used. Results: The prevalence of PON1-192, PON1-55, PON2-148 genotypes were very different between the groups but was not statistically significant. The frequency of the "SS" and "SC" genotypes of PON2-311 in patients with hepatitis and cirrhosis was higher than the control group and their Odds Ratios were statistically significant. Conclusion: The prevalence of PON2 gene "SS" and "SC" genotypes of in patients was higher than that of healthy volunteers (ORs were 3,855 and 2,404 respectively). Similarly "SS" and "SC" genotypes of in patients with cirrhosis was higher than that of patients with hepatitis (ORs were 3,436 and 2,223 respectively) These results suggested that the "SS" and "SC" genotypes of PON2 gene might cause a susceptibility for developing of hepatitis and progression of this condition to cirrhosis. It can be also speculated that "CC" genotype may be protective for the progression of disease. © TurkJBiochem.com.Item Never in mitosis gene A-related kinase 6 and aurora kinase A: New gene biomarkers in the conversion from ulcerative colitis to colorectal cancer(Spandidos Publications, 2015) Gerçeker E.; Boyacioglu S.O.; Kasap E.; Baykan A.; Yuceyar H.; Yildirim H.; Ayhan S.; Ellidokuz E.; Korkmaz M.Ulcerative colitis (UC) is an important risk factor for colorectal cancer (CRC). Histone modifications are one of the epigenetic mechanisms that may have key roles in the carcinogenesis of CRC. At present, there are no studies comparing histone modification patterns of UC and CRC in the literature. Therefore the aim of the present study was to investigate whether genes, particularly those involved in histone modification, have value in patient monitoring with regards to CRC development in UC. Key gene expressions of the histone modification enzyme were assessed and compared in CRC, UC and control groups using the RT-PCR array technique. Patients were divided into subgroups based on the extent and duration of the disease and inflammatory burden, which are considered risk factors for CRC development in UC patients. In UC and CRC groups, a significantly higher overexpression of the NEK6 and AURKA genes compared to the control group was identified. In addition, there was a significantly higher overexpression of HDAC1 and PAK1 genes in the UC group, and of HDAC1, HDAC7, PAK1 and AURKB genes in the CRC group. NEK6, AURKA, HDAC1 and PAK1 were significantly overexpressed in patients with a longer UC duration. Overexpression of AURKA and NEK6 genes was significantly more pronounced in UC patients with more extensive colon involvement. HDAC1, HDAC7, PAK1, NEK6, AURKA and AURKB are important diagnostic and prognostic markers involved in the carcinogenesis of CRC. HDAC1, PAK1, NEK6 and AURKA may be considered as diagnostic markers to be used in CRC screening for UC patients.Item Correlation of liver enzyme levels and insulin resistance in patients with non-alcoholic steatosis; [Non-alkolik steatoz hastalarinda karaciǧer enzim düzeylerinin ve insülin direncinin korelasyonu](Ankara Yildirim Beyazit University, 2019) Cekdemir D.; Gunduz M.; Celebi A.; Aslantas H.; Pekindil G.; Ayhan S.; Ellidokuz E.Objectives: To compare non-alcoholic steatosis patients according to liver enzyme levels, and to establish whether glucose metabolism and insulin resistance differ with liver enzyme levels. Materials and Methods: Patients with non-alcoholic steatosis aged between 18-65 years were included in this study. The patients were divided into two groups as steatotic patients with normal liver enzymes (NLE), and those with increased liver enzymes (ILE). The groups were compared as for parameters of glucose metabolism, and insulin resistance. Results: A total of 66 steatotic patients (40 NLE, and 26 ILE) were included in our study. Mean insulin values in the steatotic NLE, and ILE groups were 12.92±5.61, and 16.22±5.43 μU/mL respectively (p=0.012). Insulin resistance was detected in 23 (60.50%) patients in the steatotic NLE, and in 22 (88%) patients in the steatotic ILE groups (p=0.018). Metabolic syndrome was detected in 31 (77.50%) patients in the steatotic NLE, and in 15 (57.70%) patients in the steatotic ILE groups (p=0.058). Conclusion: In conclusion, we have concluded that the steatotic group with ILE had higher insulin resistance. These patients should be meticulously taken into consideration as for drug treatments which will especially decrease insulin resistance, increase both weight loss, and sensitivity to insulin resistance. Liver biopsy still retains its essential place in definitive differential diagnosis. © 2018 Ankara Medical Journal. All rights reserved.