Browsing by Author "Ercan, G"

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    Effect of Nicotine on RANKL and OPG and Bone Mineral Density
    Mizrak, S; Turan, V; Inan, S; Uysal, A; Yilmaz, C; Ercan, G
    Aim: The signaling pathway OPG/RANK/RANKL is a key in maintaining the balance between the activity of osteoblasts and osteoclasts in order to prevent bone loss. In this study, our aim was to assess the effects of long-term nicotine exposure on plasma RANKL and OPG levels, tissue RANKL and OPG immunoreactivities, and bone mineral density (BMD) scores in rats. Materials and Methods: Thirty-six Swiss Albino rats weighing 70 +/- 10 g were divided into three groups. While the controls (n = 12) were only given normal drinking water, for low-dose nicotine (LDN) group (n = 12) 0.4 mg/kg/day; for high-dose nicotine (HDN) group (n = 12), 6.0 mg/kg/day nicotine was added to drinkingwater for a year. At the end of 12th month, BMD scores were measured using an Xray absorptiometry and bone turnover was assessed by measuring plasma RANKL and OPG levels and RANKL and OPG immunoreactivities in tail vertebrae of the rats. Results: There was no statistically significant difference in BMD scores of lumbar spine and femoral regions of the nicotine groups in comparison to controls. Plasma OPG levels were found to be significantly higher in HDN group, in comparison to the controls and LDN groups (p = .001) unlike plasma RANKL levels. Tissue RANKL and OPG immunoreactivities decreased significantly in the LDN and HDN groups (p < .001, p < .01, respectively). Conclusions: The results of this study show that nicotine is not primarily responsible for the decrease in BMD frequently seen in smokers. Measuring plasma RANKL and OPG levels did not reflect tissue immunoreactivities.
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    The role of PRP and adipose tissue-derived keratinocytes on burn wound healing in diabetic rats
    Mansoub, NH; Gürdal, M; Karadadas, E; Kabadayi, H; Vatansever, S; Ercan, G
    Introduction: Diabetic burn wounds and ulcers are significant complications of diabetic patients. The aim of this study was to investigate the use of platelet rich-plasma (PRP) and/or keratinocyte-like cells (KLCs) in diabetic thermal wound rat model and to evaluate EGF, FGF-2, TGF-beta 1, COL1 alpha 2, MCP-1 and VEGF-alpha as wound healing markers at the gene expression level. Methods: In this study, we used adipose tissue as the source of mesenchymal stem cells (MSCs) and differentiated MSCs into KLCs. KLCs were characterized and transferred to the burn areas on the dorsum of streptozotocin (STZ)-induced diabetic rats. We prepared PRP from rat blood and evaluated its effect alone or in combination with KLCs. On the 3rd, 7th, 10th and 14th days after the treatment, the wound areas were measured and biopsy samples were excised from the wound areas of the KLCs and/or PRP-treated and the untreated diabetic rats to analyze the gene expression levels of the wound healing markers by qPCR. Results: We observed that, wound contraction started earlier in the PRP and/or KLCs-treated groups in comparison to the control group. However, PRP and KLCs when applied in combination showed additive affect in the wound healing. In all groups treated with KLCs and/or PRP, the gene expression levels of evaluated growth factors and COL1a2 increased, while MCP-1 levels decreased when compared to the untreated diabetic rats. In addition, the most prominent difference in qPCR results belongs to the combined PRP and KLCs-treated group. Conclusion: We demonstrated that applying PRP and KLCs in combination has a greater potential for the treatment of diabetic burn wounds.
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    Feasibility of microsurgery in burn injury and the effect of stem cell application
    Gür, E; Tiftikcioglu, YÖ; Ercan, G; Vatansever, HS; Isik, Y; Kabadayi, H; Karlitepe, A; Songür, E
    Introduction: Burn is one of the most severe traumas and can affect skin, muscle, or bone. Although early debridement and use of skin grafts have proven their benefits; in some cases, free flaps are required for early reconstruction. It is undisputed that choosing recipient vessels from unburned or undamaged zones increase the success of anastomosis. However, it may not be possible, especially in large burns. Our study aims to investigate the success of anastomosis beneath burned tissues and the effects of stem cell application. Materials and Methods: Twenty-one Wistar albino male rats weighing 250 g on average were divided equally into three Groups. In Group I, femoral artery anastomoses were done as control group. Inguinal area of 7 rats was burned as deep 2nd degree depth, and femoral artery anastomoses were done immediately beneath the burned tissues. In Group III, after burning inguinal area and making anastomoses, adipogenic stem cells were applied on the anastomosis sites. All anastomoses were checked at postoperative 3rd day. Results: One rat of Group I died in the postoperative 3rd day and one rat of Group II had a wide wound dehiscence including many of foreign body on anastomosis zone so both were excluded from the study. One anastomosis in Groups I and 3 anastomoses in both Groups II and III failed and others were successful. Statistical analyzes showed that there was no difference between groups (P < 0.05). Stem cell application was not effective on anastomosis patency in burn injury but has benefits in wound healing according to our study. Conclusion: Recipient vessels can be chosen from adjacent area to burn or inflammation zone safely for microvascular anastomosis if there is no direct burn damage on vessels.
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    A new dressing material in diabetic wounds: Wound healing activity of oleuropein-rich olive leaf extract in diabetic rats
    Samancioglu, S; Esen, A; Ercan, G; Mansoub, NH; Vatansever, S; Ince, I
    Introduction: The objective of this study was to evaluate a dressing material on ischemic wound model in diabetic rats. Study was conducted during the months of June 2012-March 2013 at Ege University in Izmir, Turkey. Materials and Methods: Sprague Dawley rats weighing 200-250 g obtained from an experimental animal production center (Saki Yenili-Ankara,Turkey) were used in this study. Animals were randomly assigned to diabetic (n= 20) and nondiabetic (n= 20) groups. After diabetes induction and wound creation, animals within each group were assigned to two wound dressing groups by a second randomization. The study was carried out on these four groups. After diabetes induction and wound creation, animals within each group (n= 10) were assigned to olive leaf extract (OLE) wound dressing and normal saline (sodium chloride, 0.9% NaCI) wound dressing groups by a second randomization. 0.9% NaCl and OLE dressing was applied to wounds once a day by the researcher. The areas of wounds were measured by Walker Formula. OLE wound dressing healed wounds faster and earlier than classic wound dressing (p< 0.05). Results: In the diabetic group; wounds closure time was found to be 24.80 +/- 1.48 in OLE wound dressing and 28.00 +/- 2.31 days in classical wound dressing. Conclusion: As a result in terms of success ratios, OLE wound dressing for wound healing in diabetic and non-diabetic wounds has been determined to be more effective in comparison with classic wound dressing method.
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