Browsing by Author "Ercan G."

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    Anti-cancer efficiency of natural killer cells differentiated from human adipose tissue-derived mesenchymal stem cells and transfected with miRNA150
    (Morion LLC, 2017) Karlitepe A.; Kabadayi H.; Vatansever S.; Gurdal M.; Gunduz C.; Ercan G.
    Aim: The aim of this study is to investigate the effects of miR150 transfection on NK-like cells differentiated from adipose tissue derived mesenchymal stem cells (AD-MSCs). Methods: NK-like cells were differentiated from AD-MSCs and activated by miR150 transfection. Transfected/non-Transfected NK-like cells were characterized by immunohistochemical and RTPCR analyzes. Apoptotic efficiency of the transfected/non-Transfected NK-like cells on pancreatic cancer cells PANC1 were determined by TUNEL and RT-PCR. Results: In miR150-Transfected cells, the increased expression of NK cell-specific genes such as GKMB, KIR2DL2, CD16, CD56, NKG2D, NKp46 and increased immunoreactivity of NK cell-specific surface marker CD314 (NKG2D) were evident. TUNEL assays showed that NK-like cells with/without transfection induced apoptosis in PANC1 cells in the same manner. The decrease in oncogene expression and the increase in the tumor suppressor gene expression in PANC1 cells upon co-culture with NK-like cells differentiated from AD-MSCs were more prominent following miRNA150 transfection. Conclusion: It was shown in vitro that NK-like cells could be obtained by differentiation from AD-MSCs and their efficiency could be increased via miR150 transfection. The results are encouraging for further clinical studies in improvement of immunotherapeutic approaches for cancer therapy. Copyright © Experimental Oncology, 2017.
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    The role of PRP and adipose tissue-derived keratinocytes on burn wound healing in diabetic rats
    (Tabriz University of Medical Sciences, 2018) Mansoub N.H.; Gürdal M.; Karadadas E.; Kabadayi H.; Vatansever S.; Ercan G.
    Introduction: Diabetic burn wounds and ulcers are significant complications of diabetic patients. The aim of this study was to investigate the use of platelet rich-plasma (PRP) and/or keratinocytelike cells (KLCs) in diabetic thermal wound rat model and to evaluate EGF, FGF-2, TGF-β1, COL1a2, MCP-1 and VEGF-α as wound healing markers at the gene expression level. Methods: In this study, we used adipose tissue as the source of mesenchymal stem cells (MSCs) and differentiated MSCs into KLCs. KLCs were characterized and transferred to the burn areas on the dorsum of streptozotocin (STZ)-induced diabetic rats. We prepared PRP from rat blood and evaluated its effect alone or in combination with KLCs. On the 3rd, 7th, 10th and 14th days after the treatment, the wound areas were measured and biopsy samples were excised from the wound areas of the KLCs and/or PRP-treated and the untreated diabetic rats to analyze the gene expression levels of the wound healing markers by qPCR. Results: We observed that, wound contraction started earlier in the PRP and/or KLCs-treated groups in comparison to the control group. However, PRP and KLCs when applied in combination showed additive affect in the wound healing. In all groups treated with KLCs and/or PRP, the gene expression levels of evaluated growth factors and COL1a2 increased, while MCP-1 levels decreased when compared to the untreated diabetic rats. In addition, the most prominent difference in qPCR results belongs to the combined PRP and KLCs-treated group. Conclusion: We demonstrated that applying PRP and KLCs in combination has a greater potential for the treatment of diabetic burn wounds. © 2018 The Author(s).