Browsing by Author "Eroglu S."

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    Identification of the variations in the CPT1B and CHKB genes along with the HLA-DQB1*06:02 allele in Turkish narcolepsy patients and healthy persons
    (Mary Ann Liebert Inc., 2014) Cingoz S.; Agilkaya S.; Oztura I.; Eroglu S.; Karadeniz D.; Evlice A.; Altungoz O.; Yilmaz H.; Baklan B.
    Background: The HLA-DQB1*06:02 allele across all ethnic groups and the rs5770917 variation between CPT1B and CHKB genes in Japanese and Koreans are common genetic susceptibility factors for narcolepsy. This comprehensive genetic study sought to assess variations in CHKB and CPT1B susceptibility genes and HLA-DQB1*06:02 allele status in Turkish patients with narcolepsy and healthy persons. Methods: CHKB/CPT1B genes were sequenced in patients with narcolepsy (n=37) and healthy persons (n=100) to detect variations. The HLA-DQB1*06:02 allele status was determined by sequence specific polymerase chain reaction. Results: The HLA-DQB1*06:02 allele was significantly more frequent in narcoleptic patients than in healthy persons (p=2×10-7) and in patients with narcolepsy and cataplexy than in those without (p=0.018). The mean of the multiple sleep latency test, sleep-onset rapid eye movement periods, and frequency of sleep paralysis significantly differed in the HLA-DQB1*06:02-positive patients. rs5770917, rs5770911, rs2269381, and rs2269382 were detected together as a haplotype in three patients and 11 healthy persons. In addition to this haplotype, the indel variation (rs144647670) was detected in the 5′ upstream region of the human CHKB gene in the patients and healthy persons carrying four variants together. Conclusion: This study identified a novel haplotype consisting of the indel variation, which had not been detected in previous studies in Japanese and Korean populations, and observed four single-nucleotide polymorphisms in CHKB/CPT1B. The study confirmed the association of the HLA-DQB1*06:02 allele with narcolepsy and cataplexy susceptibility. The findings suggest that the presence of HLA-DQB1*06:02 may be a predictor of cataplexy in narcoleptic patients and could therefore be used as an additional diagnostic marker alongside hypocretin. © 2014 Mary Ann Liebert, Inc.
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    Effects of electrical pretreatment conditions on osmotic dehydration of apple slices: Experimental investigation and simulation
    (Elsevier Ltd, 2016) YIldIz H.; Icier F.; Eroglu S.; Dagci G.
    Electrical pretreatments at 9 different conditions consisting of the combination of 3 different voltage gradients (20, 27, and 32 V/cm) and 3 different application times (10, 20, and 30 s) were applied on apple slices. Apple slices were osmotically dehydrated in 50% sucrose solution at 40 °C until their total dry matter content (TDM) reached to 40%. The effect of pretreatment conditions on the change of water loss and solid gain during osmotic dehydration was investigated, and effective diffusion coefficients were determined. The time needed to reach up to 40% TDM content was predicted by using the numeric solution of unsteady state mass transfer equations and diffusion coefficients via MATLAB code written. The electrical pretreatments reduced the osmotic dehydration time by in the range of 26-64%. The final water and solid distributions of apple slices were simulated in ANSYS. Modeling and simulation results were in good agreement with experimental data (p < 0.05). Industrial relevance Since the electrical pretreatment both shortens the osmotic dehydration time and increases the water removed per unit energy used, its application prior to osmotic dehydration processes in the commercial productions will be economical. The proposed modeling and simulation approach for assessment of the effects of electrical pretreatments on osmotic dehydration characteristics may provide valuable information on the scaling up of these conditions in the industrial scale systems. © 2016 Elsevier Ltd. All rights reserved.