Browsing by Author "Ersahin, Y"

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    Neurotoxic effect of povidone-iodine on the rat spine using a laminectomy-durotomy model
    Akcay, E; Ersahin, Y; Ozer, F; Duransoy, YK; Camlar, M; Atci, I; Yagci, A; Ozer, O
    Objective The efficacy and safety of povidone-iodine in wound dressing and irrigation of some operative cavities were established by many in vitro and in vivo experimental reports and clinical series. However, its use in defective tissue in neural structures has not been confirmed yet. The aim of the present study was to histopathologically investigate its effect on neural tissues when applied on the upper side of defective dura. Methods Wistar rats were randomly divided into two experimental groups: control and povidone-iodine groups. In the control group, durotomy was performed following laminectomy, and the spinal cord was covered with a dry sponge. In the study group, the same procedure was performed, but open duras were covered with a sponge that had been wetted with 0.1 % povidone-iodine solution. Three weeks after surgery, all experimental animals were sacrificed, and histopathological evaluations were conducted. Results Myelin changes were absent or minimal in all cases of the control group but were present as markedly increased myelin degeneration in nearly all cases in the study group. Axonal degeneration and hypoxic neuronal damage were absent in the control group, whereas they were marked in half of the study group. No statistically significant differences were established in Schwann cell proliferation, venous congestion, and lymphocytic proliferation between the two groups. Conclusions Based on the present study, 0.1 % povidone-iodine solution cannot be recommended for wound dressing for neural structures such as myelomeningocele cases because of possible damage to underlying neural tissues.
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    Comparison of The Histopathologic Outcome of Three Different Allograft Used For The Repair of Spinal Dural Defect in Rats
    Atci, IB; Demirçivi Özer, F; Mete, M; Çamlar, M; Kocaman, Ü; Akçay, E; Ersahin, Y; Öner, Ö; Küpelioglu, A
    Purpose: Repairing of the duramater is one of the major factor that effects the mortality and morbidity of patients after neurosurgical approaches. The gold standard for repairing of duramater is watertight suture or duraplasty with autografts such as pericranium and/or temporal fascia. Sometimes edges of the dura mater generally are shrunken and the watertight suture of the dura becomes impossible especially in emergency conditions. In the present study, we aimed to determine the most effective artificial dural graft in experimental dural defect in rats. Materials and Methods: Twenty eights wistar albino rats weight ranging from 280-320 grams and equal numbers of male and female were used. The animals were divided into four groups. Control (n=7 Group-1), collagen matrix graft (n=7 Group-2), cellulose graft (n=7 Group-3) and teflon graft (n=7 Group-4). Rats were sacrificed after 30 days and their damaged dura were removed and sections were taken. All histological preparations examined using light microscope. Histological analysis focused on fibroblastic activation, new capillary formation, inflammatory reaction, foreign body reaction and capsule formation and results were compared. Results: While fibroblastic activation was observed most frequently in teflon graft group, new capillary formation, inflammatory reactions and capsule formation were most frequently seen in cellulose grafts group. Conclusion: This animal model for artificial dural grafts suggest that cellulose was the most effective dural substitute for repairing of defective dura.