Browsing by Author "Fei, ZJ"

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    Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants
    Wang, L; Kaya, HB; Zhang, N; Rai, R; Willmann, MR; Carpenter, SCD; Read, AC; Martin, F; Fei, ZJ; Leach, JE; Martin, GB; Bogdanove, AJ
    Prime editing is an adaptation of the CRISPR-Cas system that uses a Cas9(H840A)-reverse transcriptase fusion and a guide RNA amended with template and primer binding site sequences to achieve RNA-templated conversion of the target DNA, allowing specified substitutions, insertions, and deletions. In the first report of prime editing in plants, a variety of edits in rice and wheat were described, including insertions up to 15 bp. Several studies in rice quickly followed, but none reported a larger insertion. Here, we report easy-to-use vectors for prime editing in dicots as well as monocots, their validation in Nicotiana benthamiana, rice, and Arabidopsis, and an insertion of 66 bp that enabled split-GFP fluorescent tagging.