Browsing by Author "Güner, A"

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    Histopathological and apoptotic changes on marine mussels Mytilus galloprovincialis (Lamark, 1819) following exposure to environmental pollutants
    Yavasoglu, A; Özkan, D; Güner, A; Katalay, S; Oltulu, F; Yavasoglu, NÜK
    Marine bivalve mussels, especially Mytilus species are an earlywarning system used for determining of damage caused by the various aquatic pollutions. In the present study, Mytilus galloprovincialis L. (black mussel) have been utilised as a biomonitoring organism to reveal environmental pollution in the Aliaga, Foca and Urla where located along the Izmir Coast of Turkey. Mussels were collected at these areas and gill and hepatopancreas (digestive gland) tissues were excised. mRNA expressions of initiator (caspase-2 and -8) and executioner (caspase -3/7-1, -3/7-2, -3/7-3 and -3/7-4) caspases of mussels tissues in areas exposed to pollution agent have been observed. TUNEL immunoreactivity in paralel to histopathological changes in both Aliaga and Foca areas were compared with Urla. This study is the first report to reveal the pollution with apoptotic expression on mussels in the coast of Turkey. (C) 2016 Elsevier Ltd. All rights reserved.
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    Novel benzimidazole derivatives: Synthesis, in vitro cytotoxicity, apoptosis and cell cycle studies
    Atmaca, H; Ilhan, S; Batir, MB; Pulat, CC; Güner, A; Bektas, H
    The aim of the study was to synthesize a new series of benzimidazole derivatives and to investigate the underlying molecular mechanisms of the potential cell cycle inhibition and apoptotic effects against a panel of selected human cancer cell lines along with HEK-293 human embryonic kidney cells. MTT assay was used to evaluate cytotoxic effects. MuseT Cell Analyzer was used to assess cell cycle progression. Annexin-V/PI staining assay was used for detecting apoptosis. All the synthesized compounds showed a significant cytotoxic effect against cancer cells with the IC50 values between 9.2 and 166.1 mu g/mL. Among the tested derivatives, compound 5 showed significant cytotoxic activity against MCF-7, DU-145 and H69AR cancer cells with the IC50 values of 17.8 +/- 0.24, 10.2 +/- 1.4 and 49.9 +/- 0.22 mu g/mL respectively. The compounds 5 was also tested on HEK-293 human embryonic kidney cells and found to be safer with lesser cytotoxicity. The results revealed that compound 5 significantly increased cell population in the G2/M-phase which is modulated by a p53 independent mechanism. Compound 5 caused an increase in the percentage of late apoptotic cells in all tested cancer cells in a concentration-dependent manner. Among all synthesized derivatives, compound 5 the bromo-derivative, showed the highest cytotoxic potential, induced G2/M cell cycle arrest and apoptotic cell death in genotypically different human cancer cells. These results suggest that compound 5 might be a promising agent for cancer therapy and further structural modifications of benzimidazole derivatives may create promising anticancer agents.
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    Cytotoxic, genotoxic, oxidative, and irritant effects of zinc pyrithione in vitro
    Güner, A; Ilhan, S
    Zinc pyrithione is an organometallic compound with antimicrobial activity used in many industrial products. In this study, cytotoxicity (cell viability, lactate dehydrogenase release, proliferation rate, and mitotic index) and genotoxicity (sister chromatid exchange and micronucleus test) of zinc pyrithione on human lymphocytes were determined. Intracellular zinc concentrations were determined by inductively coupled plasma-optical emission spectrometry. Intracellular antioxidant/oxidant status was evaluated by total antioxidant capacity and total oxidative status assays. Its irritation potential was investigated using the hen's egg test chorioallantoic membrane model. Up to the highest concentration of 1000 nmol/L, ZnPT did not cause genotoxicity and did not change the proliferation index. Above 500nmol/L, zinc pyrithione caused an increase in lactate dehydrogenase, oxidative stress, and intracellular zinc levels, while decreasing cell viability and mitotic index level. At 1000nmol/L, zinc pyrithione caused slight irritation. These results suggest that zinc pyrithione may exert toxic effects via increased oxidative stress on human cellular systems.