Browsing by Author "Güvenir, M"

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    Comparing efficiency between conventional and molecular methods for detecting Legionella pneumophilia in dental unit waterline systems
    Islam, A; Güvenir, M; Süer, K; Çetiner, S; Sanlidag, T
    The aim of our study was to detect the prevalence of Legionella pneumophilia (L. pneumophilia) in DUWL S using standard culture technique (SCT) and the real-time polymerase chain reaction (PCR) method in order to assess the risk of L. pneumophilia contamination within a dental setting. A total of 65 water samples were collected from 16 dental units and one cold water supply system from all clinical departments. L. pneumophilia could not be detected in any of the water samples using the standard SCT (0%), whereas L. pneumophilia was detected using real time PCR in three (4.6%) water samples collected from the tap system. Following the detection of L. pneumophilia, the tap systems were disinfected with surface disinfectant and water samples were recollected. The recollected water samples following disinfection were negative for L. pneumophilia once analyzed using culture and real time PCR technique. Although the culture method using BCYE media is the 'gold standard' for the detection of L. pneumophilia; Real Time PCR analysis may also be a quick, useful, and sensitive method for the detection of L. pneumophilia in order to control and prevent possible infections that may arise in the dental setting.
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    ANTIMICROBIAL ACTIVITY OF THE ESSENTIAL OIL OF THYMUS CAPITATUS AGAINST HELICOBACTER PYLORI
    Güvenir, M; Hanoglu, D; Hanodlu, A; Süer, K; Baser, HC; Yavuz, DÖ; Salidag, T
    Helicobacter pylori (H.pylori) is an infective agent and the main resourceful factor of chronic, active type B gastritis, peptic and duodenal ulcer, gastric carcinoma and mucosa-associated lymphoid tumors. Nowadays, pharmcological treatment of H. pylori infections attracts attention besides antibiotic treatment. The aim of this study is to analyse the chemical composition of the essential oils of Thymus capitatus and the antimicrobial activity against H. pylori. Aerial parts of wild-growing plant materials were collected during the flowering stage from three different locations. Essential oil composition analyses were performed simultaneously by GC-FID and GC-MS systems. The antimicrobial activity of the essential oil was tested by broth dilution method. The thymol percentage was determined as more than half of the essential oil composition of Thymus capitatus-Yedidalga (51.9%) and Thymus capitatus-Yildirim (57.1%); however, the composition of thymol was 47.2% followed by 15.1% (p-cymene), 10.0% (gamma-terpinene) and 5.7% (carvacrol) for Thymus capitatus-Bogaz/Girne. The essential oil from Thymus capitatus-Bogaz/Girne showed the lowest MIC and MBC values and the strongest bactericidal activity against H. pylori compared to the other tested essential oils. Bactericidal efficacy of essential oil of Thymus capitatus on H. pylori could have been affected due to the varied rates of major compounds. Thymus capitatus essential oil could be an alternative way for the treatment of H. pylori infections.
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    Investigation of the Correlation Between Anti-HCV Levels (S/Co) with HCV-RNA in the Diagnosis of Hepatitis C Virus (HCV) Infection
    Sanlidag, T; Akçali, S; Ecemis, T; Süer, K; Erbay Dündar, P; Arikan, A; Güvenir, M; Güler, E
    Detection of borderline and/or low positive anti-HCV results by enzyme immunoassay (EIA) leads to severe problems in routine laboratories and needs confirmation with nucleic acid amplification tests which can increase the cost. In EIA tests, if the ratio of sample to cut-off (S/Co) is >= 1, the sample is accepted as positive according to the manufacturers' instructions. Although over the last decade the application of S/Co values have also applied to HCV-RNA readings, the current study aims to determine whether the S/Co value is adequate and applicable for the anti-HCV EIA test, and to determine whether a correlation exists between HCV-RNA and HCV infections. A total of 658 cases (402 female, 256 male; mean age: 49.4 +/- 17.0 years) who were found anti-HCV positive between January 2011-July 2013 were included in the study. Anti-HCV tests were performed by chemiluminescent EIA (Architect i2000SR, Abbott, USA and LiaisonXL Murex, DiaSorin, Italy) and HCV-RNA by real-time PCR (Cobas Ampliprep/Cobas TaqMan HCV, Roche, USA). The mean S/Co value of the cases was 7.3 +/- 4.8 (range: 1.00-17.59) and mean HCV-RNA value was 2.3x10(5) +/- 2.1x10(6) copies/ml. When the anti-HCV S/Co value of varying ranges was compared with HCV-RNA readings a particular trend was noted. In the anti-HCV S/Co values of 1.0-4.0; 4.1-7.0; 7.1-10.0; 10.1-13.0; 13.1-16.0 and (3)16.1, HCV-RNA positivity rates were detected as 1.9%, 24.7%, 37.1%, 46.7%, 56.4% and 75%, respectively. Statistical analysis indicated an intermediate positive correlation (r=0.454) between anti-HCV ve HCV-RNA readings (p=0.000). An adequate S/Co value was accepted as 5.0 based on the ROC analysis, and this value gave a performance confidence level of 95.6% when determining whether a patient is HCV positive. Based on the data of this study it became evident that further EIA testing is not required if the S/Co value is >= 5.0, however if the S/Co value is less than 5.0, then further clinical analysis and revaluation of the patient is required.