Browsing by Author "Gumus, BH"
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Item Renal functions of enuretic and nonenuretic children: Hypernatriuria and kaliuresis as causes of nocturnal enuresisVurgun, N; Gumus, BH; Ece, A; Ari, Z; Tarhan, S; Yeter, MObjective: The aim of this study was to investigate the pathophysiologic cause(s) of primary nocturnal enuresis. Therefore, electrolyte concentrations of urine specimens were evaluated in the morning, and alterations compared between enuretics and nonenuretics. Methods and Patients: First morning urine specimens of 27 enuretics and 21 nonenuretic subjects fed the same diet were collected, and urinary electrolytes were measured. The urinary Ca/Cr ratio, tubular reabsorption of phosphorus (TRP) and fractional sodium (FE Na%) and potassium excretions (FE K%) were determined for patients and controls. Results: There was no significant difference in the Ca/Cr ratio and TRP between patients and controls, but enuretic patients had significantly higher FE Na% and FE K% values than controls (p < 0.001). There were significant positive correlations between FE Na% and (r = 0.81, p < 0.001) FE K% and the frequency of bedwetting, respectively, among enuretic patients (r = 0.54, p < 0.001). Conclusion: Since Na and K excretion of enuretic patients was higher than in nonenuretics, it can be concluded that there may be a benign hereditary and/or postural renal tubular handling disorder of Na and K in enuretic children.Item Histologic and physiologic analysis of the relationship between the dorsal nerve of the penis and the corpus cavernosum on a rat model. A complementary pathway on the innervation of penile erection?Kozacioglu, Z; Vatansever, HS; Onal, T; Kutlu, N; Ozel, F; Gunlusoy, B; Gumus, BHAim The dorsal nerve of the penis (DNP) is the terminal branch of the pudendal nerve which is responsible for the somatic innervation of the penis. This study aims to outline any direct role of the DNP in the hemodynamics of erection histologically and physiologically. Materials and Methods Fifteen Wistar albino rats were sorted into the electrical activity (n = 6), intracavernous pressure (n = 4), and control (n = 5) groups. The dorsal nerve was electrostimulated and the simultaneous changes in intracavernous pressure and smooth muscle activity were recorded. Penile tissues were collected, fixed, and sectioned, the slides were stained with either hematoxylin-eosin for morphological evaluation or using the indirect immunoperoxidase technique to analyze the distributions of eNOS, iNOS, and nNOS. Results During electrostimulation, there was a simultaneous statistically significant decrease in the electrical activity inside the corpora in electromyography and an increase in intracavernous pressure. eNOS and iNOS immunoreactivities were higher in the study group than in the control group. nNOS immunoreactivity was moderate in both study and control groups. Conclusion Some fibers in the dorsal nerve of penis continue into the corpora cavernosa through the tunica albuginea and have an active, direct role in the hemodynamic process of erection, which may be complementary to the main route of innervation.Item Association between severity of lower urinary tract symptoms, erectile dysfunction and metabolic syndromeDemir, O; Akgul, K; Akar, Z; Cakmak, O; Ozdemir, I; Bolukbasi, A; Can, E; Gumus, BHIntroduction. The purpose of this study was to investigate the association between severity of lower urinary tract symptoms (LUTS), erectile dysfunction (ED) and metabolic syndrome. Methods. Our study population included a consecutive series of 190 patients with LUTS (International Prostate Symptom Score-IPSS 7) with or without manifestations of the metabolic syndrome. The diagnoses of diabetes mellitus and hypertension were obtained from the patient's medical history. Data on blood pressure, waist measure, body height and weight were collected and body mass index were calculated. Patients were assessed based on the International Index of Erectile Function (IIEF) for ED and IPSS and IPSS-Quality of Life for LUTS. Blood samples were drawn from fasting patients to determine, fasting blood glucose (FBG), triglycerides, HDL-cholesterol and serum total testosterone levels. Results. In severe LUTS patient group, IIEF erectile function domain scores were significantly lower than moderate LUTS patient group (p0.05). Multiple logistic regression analysis confirmed that presence of ED was the most predictor of severe LUTS. The prevalence of metabolic syndrome was higher in patients with severe LUTS (26%vs. 46%, p=0.009). The severe form of the LUTS was significantly correlated with waist circumference 102cm (p0.05), blood pressure 130/85mmHg (p0.05) and FBG 110mg/dl (p0.01). Conclusion. Obesity, high plasma level of FBG and hypertension constitute risk factors for the development of severe LUTS. Metabolic syndrome may play a key role in the pathogenesis in both ED and LUTS. Presence of ED is the most predictor of severe LUTS.Item Sensitive and reliable lab-on-paper biosensor for label-free detection of exosomes by electrochemical impedance spectroscopySazaklioglu, SA; Torul, H; Tamer, U; Ensarioglu, HK; Vatansever, HS; Gumus, BH; Çelikkan, HA new, sensitive, and cost-effective lab-on-paper-based immunosensor was designed based on electrochemical impedance spectroscopy (EIS) for the detection of exosomes. EIS was selected as the determination method since there was a surface blockage in electron transfer by binding the exosomes to the transducer. Briefly, the carbon working electrode (WE) on the paper electrode (PE) was modified with gold particles (AuPs@PE) and then conjugated with anti-CD9 (Anti-CD9/AuPs@PE) for the detection of exosomes. Variables involved in the biosensor design were optimized with the univariate mode. The developed method presents the limit of detection of 8.7 x 102 exosomes mL-1, which is lower than that of many other available methods under the best conditions. The biosensor was also tested with urine samples from cancer patients with high recoveries. Due to this a unique, low-cost, biodegradable technology is presented that can directly measure exosomes without labeling them for early cancer or metastasis detection.Item Electrochemically generated paper SERS substrate for detection of exosome in urine samplesKayis, EÇ; Torul, H; Sazaklioglu, SA; Çelikkan, H; Ensarioglu, HK; Gumus, BH; Vatansever, HS; Tamer, UHere, a single-drop paper-based surface-enhanced Raman spectroscopy (SERS) immunoassay was developed to pave the way for monitoring exosome numbers for the early diagnosis of prostate cancer. Exosomes are nanosized (40-150 nm) membrane vesicles that provide intercellular communication. In our work, we offer a new paper SERS substrate for exosome detection in urine. We initially electrochemically deposited nanostar-shaped gold nanoparticles (AuNPs) on the working electrode to crate the paper SERS substrates. Then we functionalized them with 11-mercaptoundecanoic acid (11-MUA) and conjugated them with anti-CD9 antibodies. After capturing exosomes, the sandwich immunoassay structure was created by using gold nanorods (AuNRs) modified with 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) as a Raman tag. The SERS signal intensities of DTNB molecules at 1330 cm-1 were monitored to determine the exosome concentration. Each step occurred in only one drop of solution or sample. The developed single-drop paper-based SERS immunoassay exhibited a linear range from 1.0 x 103 to 1.0 x 109 exosome particles/mL with correlation coefficients (R2) of 0.9903. The limit of detection (LOD) was found as 9.9 x 101 exosome particles/mL. The developed system was tested with clinical urine samples from patients with benign prostatic hyperplasia, prostatitis, prostate cancer, and healthy individuals. The obtained results were compared with the exosome particle numbers in these samples determined by an enzyme-linked immunosorbent assay (ELISA) method and the accuracy of the system was evaluated with an average recovery value of 96.7 %. The developed biosensor system enables highly sensitive detection of exosomes in low-volume urine samples. The usage of a paper membrane as a SERS substrate, combined with the electrochemical deposition of gold nanoparticles, provides an eco-friendly and cost-effective solution, enabling wider use and applications.Item A Retrospective Analysis of 83 Patients with Testicular Mass Who Underwent Testis-Sparing Surgery: The Eurasian Uro-oncology Association Multicenter StudyKeske, M; Canda, AE; Karadag, MA; Çiftçi, H; Erturhan, S; Kactan, C; Soytas, M; Özkaya, F; Ozbey, I; Ordek, E; Atmaca, AF; Yildirim, A; Sahin, S; Colakoglu, Y; Boylu, U; Erol, B; Caskurlu, T; Kiremit, MC; Cakici, OU; Sonmez, G; Kiliçarslan, H; Akbulut, Z; Kaygisiz, O; Bedir, S; Vuruskan, H; Bozkurt, YE; Aydin, HR; Oguz, U; Basok, EK; Gumus, BH; Tuncel, A; Aslan, Y; Hamidi, N; Müslümanoglu, AY; Dinçer, M; Balbay, D; Albayrak, S; Laguna, MPIntroduction: Herein, we analyzed the histopathological, oncological and functional outcomes of testis-sparing surgery (TSS) in patients with distinct risk for testicular cancer. Methods: This is a multicenter retrospective study on consecutive patients who underwent TSS. Patients were categorized in high- or low-risk testicular germ cell tumor (TGCT) according to the presence/absence of features compatible with testicular dysgenesis syndrome. Histology was categorized per size and risk groups. Results: TSS was performed in 83 patients (86 tumors) of them, 27 in the high-risk group. Fifty-nine patients had a non-tumoral contralateral testis present. Sixty masses and 26 masses were benign and TGCTs, respectively. No statistical differences were observed in mean age (30.9 +/- 10.32 years), pathological tumor size (14.67 +/- 6.7 mm) between risk groups or between benign and malignant tumors (p = 0.608). When categorized per risk groups, 22 (73.3%) and 4 (7.1%) of the TSS specimens were malignant in the high- and low-risk patient groups, respectively. Univariate analysis showed that the only independent variable significantly related to malignant outcome was previous history of TGCT. During a mean follow-up of 25.5 +/- 22.7 months, no patient developed systemic disease. Local recurrence was detected in 5 patients and received radical orchiectomy. Postoperative testosterone levels remained normal in 88% of those patients with normal preoperative level. No erectile dysfunction was reported in patients with benign lesions. Conclusion: TSS is a safe and feasible approach with adequate cancer control, and preservation of sexual function is possible in 2/3 of patients harboring malignancy. Incidence of TGCT varies extremely between patients at high and low risk for TGCT requiring a careful consideration and counseling.